Nonpolyposis colorectal cancer- HNPCC kit

HNPCC (Hereditary non-polyposis colorectal cancer) is an autosomal dominant genetic condition predisposing to the early development of various cancers including those of colon, rectum, endometrium, etc. This disease is due to germline mutations in genes encoding proteins responsible for the repair of DNA replication errors, which are referred to as DNA mismatch repair (MMR) genes.

The microsatellite instability phenotype, characterized by widespread somatic alterations in the length of associated tumor and in 10-15% sporadic gastric and colon cancer.

HNPCC kit 1 – FL allows microsatellites instability analysis in tumor-associated HNPCC and in sporadic colon and stomach cancer by pentaplex PCR and then amplicon capillary electrophoresis by automatic sequencer.

L

In the kit are provided five mononucleotide “almost-monomorphic” markers (BAT 25, BAT 26, NR21, NR22, NR24) dyed with fluorophores 6-FAM, HEX and TAMRA, for ABI Prism Instrument (Applied Biosystems) use.