{"id":26915,"date":"2025-10-06T11:55:39","date_gmt":"2025-10-06T11:55:39","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=26915"},"modified":"2026-03-23T15:40:11","modified_gmt":"2026-03-23T15:40:11","slug":"illumina-dna-prep-with-exome-2-5-enrichment","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/illumina-dna-prep-with-exome-2-5-enrichment\/","title":{"rendered":"Illumina DNA Prep with Exome 2.5 Enrichment"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:wght@400;700&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n        line-height: 1.6 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technology, combining library preparation by tagmentation with capture-based enrichment, sequencing, and bioinformatics analysis, enabling a single workflow.<\/p>\n    <\/div>\n\n    <!-- Imagen Workflow Actualizada -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/03\/workfloweng.webp\" alt=\"Illumina Exome Workflow\" class=\"dlw-prod-img\" title=\"\">\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <h3>Operating Principle<\/h3>\n    <p>The workflow uses magnetic bead-linked transposomes (eBLT) for rapid and uniform tagmentation. Following index PCR, a hybrid capture enrichment step is performed using the <em>Twist Bioscience for Illumina Exome 2.5<\/em> panel. Once the process is complete, the libraries are ready for sequencing. Additionally, secondary and tertiary analyses are seamlessly handled by the DRAGEN&#x2122; and Emedgene modules, respectively.<\/p>\n\n    <!-- Imagen Librer\u00edas Actualizada -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/03\/libreria-eng.webp\" alt=\"Illumina Library Preparation\" class=\"dlw-prod-img\" title=\"\">\n\n    <h3>Clinical Applications<\/h3>\n    <p>Whole exome sequencing (WES) is currently an essential tool in clinical practice for identifying genomic alterations with diagnostic and\/or therapeutic relevance. Compared to whole genome sequencing, it offers lower costs and optimal coverage of the coding regions of the human genome, facilitating more precise diagnoses and informed medical decision-making.<\/p>\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\"><strong>Improved coverage:<\/strong> optimized panel to cover clinically relevant regions according to RefSeq, CCDS, ACMG, COSMIC, and OMIM databases.<\/span>\n        <span class=\"dlw-list-item\"><strong>Content flexibility:<\/strong> option to add a panel to capture the mitochondrial genome or other complementary panels, easily adapting to all laboratory needs.<\/span>\n        <span class=\"dlw-list-item\"><strong>Comprehensive solution<\/strong> with support across all protocol steps through a single vendor.<\/span>\n        <span class=\"dlw-list-item\"><strong>Uniformity:<\/strong> consistent sequencing performance across libraries.<\/span>\n        <span class=\"dlw-list-item\"><strong>Scalability<\/strong> across multiple Illumina sequencing systems.<\/span>\n    <\/div>\n\n    <h3>Intended Audience\/User<\/h3>\n    <p>Molecular diagnostic laboratories working with human genomic DNA samples to diagnose genetic diseases using whole exome techniques.<\/p>\n\n    <!-- Aspectos Clave -->\n    <h2>Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span><strong>Curated and updated coverage:<\/strong> includes RefSeq, CCDS, ACMG, COSMIC, and OMIM reference regions, with high coverage of clinically relevant variants according to ClinVar and ACMG databases.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span><strong>Efficient library preparation:<\/strong> eBLT technology and integrated normalization for inputs \u2265 50 ng.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span><strong>Complete workflow:<\/strong> from sample to final report, compatible with automated analysis using DRAGEN&#x2122; and Emedgene.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span><strong>Scalability and cost-efficiency:<\/strong> allows processing a variable number of samples per batch depending on the sequencing platform used and the desired sequencing depth.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span><strong>Expanded content:<\/strong> includes a panel to study the complete mitochondrial genome and the option for customized complementary panels.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span><strong>Validated automation platforms.<\/strong><\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones Desplegables) -->\n    <h2>Ordering Information<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Kit Components<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">20077595<\/td>\n                        <td width=\"70%\">Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set B (96 samples, 12\u2011plex)<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">20077596<\/td>\n                        <td>Illumina DNA Prep with Exome 2.5 Enrichment, (S) Tagmentation Set D (96 samples, 12\u2011plex)<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">20091654<\/td>\n                        <td>UD Indexes (96 indexes\/96 samples): Set A<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">20091656<\/td>\n                        <td>UD Indexes (96 indexes\/96 samples): Set B<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">20091658<\/td>\n                        <td>UD Indexes (96 indexes\/96 samples): Set C<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">20091650<\/td>\n                        <td>UD Indexes (96 indexes\/96 samples): Set D<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Additional Reagents (Extraction from Blood)<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">20018706<\/td>\n                        <td width=\"70%\">Flex Lysis Reagent Kit (96 reactions). <em>Required for direct input of unextracted peripheral blood.<\/em><\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Optional Enrichment Panels<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">20093180<\/td>\n                        <td width=\"70%\">Twist Bioscience for Illumina Mitochondrial Panel (96 samples, 12\u2011plex). Full coverage of chrM (16,659 bp; 37 genes).<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Inquire<\/td>\n                        <td>Illumina Custom Enrichment Panel v2, custom spike-in to expand content or deepen coverage in specific genomic regions.<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Comprehensive solution for whole exome sequencing with Illumina technology, combining library preparation by tagmentation with capture-based enrichment, sequencing, and bioinformatics analysis, enabling a single workflow. Detailed Description Operating Principle The workflow uses magnetic bead-linked transposomes (eBLT) for rapid and uniform tagmentation. Following index PCR, a hybrid capture enrichment step is performed using the Twist Bioscience [&hellip;]<\/p>\n","protected":false},"featured_media":26916,"template":"","tecnologias":[36],"marcas":[232],"familias":[283,297,425],"class_list":["post-26915","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-illumina-en","familias-molecular-genetics","familias-exomes","familias-hereditary_and_or_rare_diseases"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/26915","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/26915\/revisions"}],"predecessor-version":[{"id":26918,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/26915\/revisions\/26918"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/26916"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=26915"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=26915"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=26915"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=26915"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}