{"id":27085,"date":"2026-04-13T08:20:16","date_gmt":"2026-04-13T08:20:16","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27085"},"modified":"2026-04-13T08:23:34","modified_gmt":"2026-04-13T08:23:34","slug":"twist-long-read-capture-chemistry-with-alliance-panels-dark-genes-and-pharmacogenomics","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/twist-long-read-capture-chemistry-with-alliance-panels-dark-genes-and-pharmacogenomics\/","title":{"rendered":"Twist long-read capture chemistry with Alliance panels (dark genes and pharmacogenomics)"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:wght@400;700&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper 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!important; text-transform: uppercase !important; }\n        .dlw-table td:nth-of-type(1)::before { content: \"Attribute\" !important; }\n        .dlw-table td:nth-of-type(2)::before { content: \"Detail\" !important; }\n    }\n<\/style>\n\n<!-- ==========================================\n     CONTENIDO UNIFICADO Y BLINDADO\n     ========================================== -->\n<div class=\"dlw-prod-wrapper\">\n\n    <!-- Descripci\u00f3n Corta (Lead Box) -->\n    <div class=\"dlw-lead-box\">\n        <p>Target enrichment solution for long-read sequencing that combines preconfigured panels and customizable options to study complex genomic regions with high uniformity, balanced coverage, and precise analysis of SNPs, indels, and structural variants.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <h3>Operating Principle<\/h3>\n    <p>The workflow integrates Twist&#8217;s targeted enrichment technology with long-read sequencing to efficiently capture genomic regions of interest at scale. Probes have been optimized to promote uniform capture and high sequencing efficiency, even in complex or hard-to-map regions.<\/p>\n\n    <!-- Imagen Workflow -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/workflow-eng.webp\" alt=\"Twist Long-Read Workflow\" class=\"dlw-prod-img\" title=\"\">\n\n    <p>The protocol is optimized for long-fragment enrichment and allows working with both predefined panels and fully customizable, scalable designs.<\/p>\n\n    <h3>Clinical Applications<\/h3>\n    <p>This solution is particularly relevant in clinically focused studies where short-read technologies face limitations, such as in genes with pseudogenes, repetitive regions, or segmental duplications.<\/p>\n    <p>Twist Bioscience offers two commercial panels with different applications:<\/p>\n    \n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The Twist Alliance Long-Read PGx panel is tailored for the characterization of pharmacogenomic genes, including highly complex genes like HLA and CYP2D6, as well as the complete mitochondrial genome for simultaneous detection of heteroplasmy.<\/span>\n        <span class=\"dlw-list-item\">The Twist Alliance Dark Genes panel provides comprehensive coverage of 389 clinically relevant genes located in &#8216;dark&#8217; regions of the genome\u2014regions that are difficult or impossible to fully resolve using short-read sequencing. The genes included in this panel are involved in multiple clinical areas, including cardiology, neurology, immunodeficiencies, ophthalmology, and cancer.<\/span>\n    <\/div>\n\n    <!-- Imagen Panels -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen1.webp\" alt=\"Twist Alliance Long-Read Panels\" class=\"dlw-prod-img\" title=\"\">\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Enables better characterization of complex regions, maintaining a scalable strategy at a more accessible cost than whole genome sequencing.<\/span>\n        <span class=\"dlw-list-item\">The combination of capture enrichment and long-read sequencing allows for highly accurate variant calling for SNPs, indels, and SVs, as well as unambiguous haplotype resolution and phasing.<\/span>\n        <span class=\"dlw-list-item\">In pharmacogenomics, this is especially relevant for star allele assignment and the correct interpretation of complex loci; in the so-called &#8216;dark genes&#8217;, it improves coverage in regions that typically present gaps or coverage valleys with short-read WGS.<\/span>\n        <span class=\"dlw-list-item\">Uniform coverage of target regions such as CYP2D6, HLA-B, GBA, LPA, SMN1, and PMS2, compared to the variable coverage and gaps seen in short-read WGS.<\/span>\n        <span class=\"dlw-list-item\">Compatible with PacBio and Oxford Nanopore systems.<\/span>\n    <\/div>\n\n    <h3>Intended Audience \/ User<\/h3>\n    <p>Aimed at genetics, clinical genomics, pharmacogenomics, and translational research laboratories needing to study complex regions through targeted long-read panels, with the ability to analyze large cohorts and multiplex multiple samples in a single run.<\/p>\n\n    <!-- Aspectos Clave -->\n    <h2>Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Targeted enrichment for long-read sequencing with preconfigured panels (LR PGx and LR Dark Genes) and fully customizable options.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Optimized probes to achieve high uniformity, sequencing efficiency, and balanced coverage in hard-to-sequence or hard-to-map regions.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Precise detection of SNPs, indels, and structural variants (SVs).<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Haplotype resolution and long-range phasing without the need for trios.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Prominent application in pharmacogenomics, including the characterization of complex loci such as CYP2D6 and HLA genes.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Application in medically relevant genes from &#8216;dark&#8217; regions, such as GBA, SMN1\/2, and PMS2.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Design oriented towards scalable and cost-effective studies in large cohorts.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones Desplegables) -->\n    <h2>Ordering Information<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Twist Alliance Long-Read PGx Panel<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">Panel size<\/td>\n                        <td width=\"70%\">2 Mb<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Content<\/td>\n                        <td>50 genes<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Application<\/td>\n                        <td>Focused on relevant pharmacogenomic genes, including CYP genes, HLA, and the complete mitochondrial genome.<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Twist Alliance Dark Genes Panel<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">Panel size<\/td>\n                        <td width=\"70%\">22 Mb<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Content<\/td>\n                        <td>389 clinically relevant, hard-to-analyze genes, such as: GBA, SMN1\/2, PMS2<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <div class=\"dlw-footer-note\">\n        Possibility to develop customized and scalable panels tailored to each laboratory&#8217;s needs.\n    <\/div>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Target enrichment solution for long-read sequencing that combines preconfigured panels and customizable options to study complex genomic regions with high uniformity, balanced coverage, and precise analysis of SNPs, indels, and structural variants. Detailed Description Operating Principle The workflow integrates Twist&#8217;s targeted enrichment technology with long-read sequencing to efficiently capture genomic regions of interest at scale. 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