{"id":27097,"date":"2026-04-13T08:41:53","date_gmt":"2026-04-13T08:41:53","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27097"},"modified":"2026-04-13T08:45:00","modified_gmt":"2026-04-13T08:45:00","slug":"ngs-methylation-detection-system-twist-bioscience","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/ngs-methylation-detection-system-twist-bioscience\/","title":{"rendered":"NGS Methylation Detection System | Twist Bioscience"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n 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The solution is designed for epigenetics, oncology, and the discovery of differentially methylated regions.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <h3>Operating Principle<\/h3>\n    <p>This is an end-to-end sample preparation and enrichment solution geared towards methylation analysis.<\/p>\n    <p>The workflow combines an enzymatic conversion of unmethylated cytosines, followed by capture-based enrichment using Twist probes and optimized hybridization reagents. Compared to chemical bisulfite treatment, this enzymatic approach is less harsh on DNA, requires less sample input, and generates higher quality libraries with better yield. Following PCR, the nucleotide representation is equivalent to that of bisulfite-treated DNA, allowing it to be integrated into established bioinformatics pipelines.<\/p>\n\n    <!-- Imagen 1 Workflow -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Workflow-twist-metilacion-eng.webp\" alt=\"Twist methylation workflow\" class=\"dlw-prod-img\" title=\"\">\n\n    <h3>Clinical Applications<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Geared towards methylation profiling applications in research related to cancer metastasis, human development, and functional genomics. Especially relevant in liquid biopsy studies due to its high compatibility with this type of samples.<\/span>\n        <span class=\"dlw-list-item\">It can also be used for the identification of differentially methylated cytosines or regions (DMCs\/DMRs) with potential functional, diagnostic, or prognostic value.<\/span>\n    <\/div>\n\n    <h3>Benefits<\/h3>\n    <p>The system stands out for providing a less damaging library preparation, with better mapping efficiency, higher GC uniformity, and overall improved sequencing metrics. In addition, it incorporates the Twist Methylation Enhancer, a specific blocker for post-conversion samples that reduces off-target capture by up to half without significantly affecting other metrics.<\/p>\n\n    <h3>Technology Used<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Compatible with broad commercial panels, targeted oncology panels, and custom solutions that fit the needs of each laboratory. \n            <div class=\"dlw-sub-list\">\n                <span class=\"dlw-sub-list-item\">Twist Human Methylome Panel: 3.98M CpGs and 123 Mb of genomic content. \n                    <div class=\"dlw-sub-list\" style=\"margin-left: 1.5em !important;\">\n                        <span class=\"dlw-sub-list-item\" style=\"color: #666 !important; font-size: 0.9em !important;\">This panel identifies 84.2% of the genome&#8217;s CpG islands and significantly expands coverage compared to average microarrays, providing a greater capacity to analyze DMRs and adjacent regions.<\/span>\n                    <\/div>\n                <\/span>\n            <\/div>\n        <\/span>\n    <\/div>\n\n    <!-- Imagen 2 Comparativa -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen-comparativa-con-array-eng.webp\" alt=\"Array comparison image\" class=\"dlw-prod-img\" title=\"\">\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">\n            <div class=\"dlw-sub-list\">\n                <span class=\"dlw-sub-list-item\">Twist Alliance Pan-cancer Panel: ~126,000 CpG sites, ~12,000 DMRs, 31 cancer types, and 47 TCGA entities.<\/span>\n                <span class=\"dlw-sub-list-item\">Custom panels<\/span>\n            <\/div>\n        <\/span>\n    <\/div>\n\n    <h3>Intended Audience\/User<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Aimed at laboratories and groups specializing in epigenetics, molecular oncology, translational genomics, and NGS sequencing that need to study methylation in a targeted manner, with high capture efficiency and a less harsh alternative to bisulfite-based workflows.<\/span>\n    <\/div>\n\n    <!-- Aspectos Clave -->\n    <h2>Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Comprehensive solution for targeted NGS methylation analysis with enzymatic conversion, capture enrichment, and optimized reagents.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Alternative to bisulfite with less DNA damage and better library quality.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Compatible with established bioinformatics analysis pipelines for methylation sequencing.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Possibility of incorporating custom panels to perform tailored studies.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones Desplegables) -->\n    <h2>Ordering Information<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Preparation and enrichment reagents<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">103496<\/td>\n                        <td width=\"70%\">Library preparation and target enrichment reagents, 96 samples<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Twist Human Methylome Panel<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">105517<\/td>\n                        <td width=\"70%\">2 captures<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">105520<\/td>\n                        <td>12 captures<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">105521<\/td>\n                        <td>96 captures<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Other Panels<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">Inquire<\/td>\n                        <td width=\"70%\">Twist Alliance Pan-cancer Methylation Panel<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Comprehensive system for NGS methylation analysis combining enzymatic conversion, target enrichment, and high-performance methylation panels to identify methylated regions with greater efficiency, less DNA damage, and optimized sequencing metrics. The solution is designed for epigenetics, oncology, and the discovery of differentially methylated regions. Detailed Description Operating Principle This is an end-to-end sample preparation and enrichment [&hellip;]<\/p>\n","protected":false},"featured_media":27071,"template":"","tecnologias":[36],"marcas":[242],"familias":[464,292,427,283,285],"class_list":["post-27097","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-twist-bioscience-en","familias-tumor-types","familias-epigenomics","familias-methylation_studies_by_ngs","familias-molecular-genetics","familias-omics"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27097","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27097\/revisions"}],"predecessor-version":[{"id":27099,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27097\/revisions\/27099"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27071"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27097"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27097"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27097"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27097"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}