{"id":27120,"date":"2026-04-13T09:03:34","date_gmt":"2026-04-13T09:03:34","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27120"},"modified":"2026-04-13T09:22:02","modified_gmt":"2026-04-13T09:22:02","slug":"twist-enzymatic-fragmentation-for-pcr-free-whole-genome-sequencing-workflow","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/twist-enzymatic-fragmentation-for-pcr-free-whole-genome-sequencing-workflow\/","title":{"rendered":"Twist Enzymatic Fragmentation for PCR-Free Whole Genome Sequencing Workflow"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        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preparation kit designed for demanding WGS applications, featuring adjustable enzymatic fragmentation, high yield, and more uniform coverage even from low-input or degraded DNA. The documentation highlights its utility in complex samples, including severely degraded FFPE, and its strong performance in AT- and GC-rich regions.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <!-- Imagen 1 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen-workflow-eng.webp\" alt=\"Twist TrueAmp Workflow\" class=\"dlw-prod-img\" title=\"\">\n\n    <h3>Operating Principle<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">This library preparation solution is designed to improve one of the most critical stages of the NGS workflow: generating robust, uniform, and high-yield libraries from challenging samples.<\/span>\n        <span class=\"dlw-list-item\">The kit incorporates adjustable enzymatic fragmentation, allowing the insert size to be modulated based on the needs of the sequencing workflow.<\/span>\n        <span class=\"dlw-list-item\">Additionally, an enhanced polymerase is used to enable more uniform amplification across the genome.<\/span>\n    <\/div>\n\n    <!-- Imagen 2 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen1-1.webp\" alt=\"Twist TrueAmp Performance\" class=\"dlw-prod-img\" title=\"\">\n\n    <h3>Clinical Applications<\/h3>\n    <p>In the context of clinically relevant WGS, the kit is especially relevant for laboratories working with compromised quality samples or limited DNA amounts, where library preparation can significantly impact final data quality. The documentation specifically points out WGS applications and highlights its ability to generate libraries from just 5 ng of DNA derived from severely degraded FFPE samples (DIN &lt; 2.2). This performance makes it an attractive option for workflows aiming to maximize genomic information recovery in complex samples and reduce the impact of technical bias on coverage.<\/p>\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The workflow allows the PCR step to be eliminated in final library preparation, positioning it as a PCR-free option for WGS.<\/span>\n        <span class=\"dlw-list-item\">Allows adjusting the insert size through &#8220;tunable&#8221; enzymatic fragmentation, consistently achieving higher yields and greater uniformity.<\/span>\n        <span class=\"dlw-list-item\">A protocol geared towards reducing coverage loss in GC-rich or GC-poor regions, which is especially relevant in WGS when a more balanced genome representation and better variant recovery are required.<\/span>\n    <\/div>\n\n    <h3>Intended Audience \/ User<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Aimed at laboratories performing whole genome sequencing that need robust library preparation for scarce, degraded, or variable-quality DNA, with a special focus on optimizing coverage uniformity, library yield, and the recovery of useful data from complex samples.<\/span>\n    <\/div>\n\n    <!-- Aspectos Clave -->\n    <h2>Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Library preparation for WGS and other NGS applications with adjustable enzymatic fragmentation.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Strong performance with low inputs and degraded DNA, including severely degraded FFPE samples.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>High fidelity in AT- and GC-rich regions, with improved coverage uniformity.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Relevant for WGS workflows requiring a more balanced genome representation and improved variant detection.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Imagen 3 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen2.webp\" alt=\"AT and GC Fidelity Twist TrueAmp\" class=\"dlw-prod-img\" title=\"\">\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones Desplegables) -->\n    <h2>Ordering Information<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Twist TrueAmp Library Preparation<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">116480<\/td>\n                        <td width=\"70%\">16 samples<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">116481<\/td>\n                        <td>96 samples<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Twist TrueAmp Polymerase Mix<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">116471<\/td>\n                        <td width=\"70%\">Twist TrueAmp Polymerase Mix, 16 reactions<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">116472<\/td>\n                        <td>Twist TrueAmp Polymerase Mix, 96 reactions<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Twist PCR-Free WGS Library Preparation Kit<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">128388<\/td>\n                        <td width=\"70%\">16 samples<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">128389<\/td>\n                        <td>96 samples<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>NGS library preparation kit designed for demanding WGS applications, featuring adjustable enzymatic fragmentation, high yield, and more uniform coverage even from low-input or degraded DNA. The documentation highlights its utility in complex samples, including severely degraded FFPE, and its strong performance in AT- and GC-rich regions. Detailed Description Operating Principle This library preparation solution is [&hellip;]<\/p>\n","protected":false},"featured_media":27119,"template":"","tecnologias":[36],"marcas":[242],"familias":[283,426,425],"class_list":["post-27120","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-twist-bioscience-en","familias-molecular-genetics","familias-genomes","familias-hereditary_and_or_rare_diseases"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27120","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27120\/revisions"}],"predecessor-version":[{"id":27121,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27120\/revisions\/27121"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27119"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27120"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27120"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27120"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27120"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}