{"id":27164,"date":"2026-04-13T11:34:44","date_gmt":"2026-04-13T11:34:44","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27164"},"modified":"2026-04-13T11:40:48","modified_gmt":"2026-04-13T11:40:48","slug":"hla-subito-ngstype","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/hla-subito-ngstype\/","title":{"rendered":"HLA-SuBiTo NGStype\u00ae"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n        line-height: 1.6 !important;\n        background-color: transparent !important;\n        padding: 0 !important;\n        max-width: 100% !important;\n        text-align: left !important;\n        display: block !important;\n        font-size: 14px !important; 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}\n        .dlw-check-list { grid-template-columns: 1fr !important; padding: 1.5em !important; }\n        .dlw-prod-img { max-width: 100% !important; }\n        \n        \/* Apilar Split Layout en m\u00f3vil *\/\n        .dlw-text-image-split {\n            flex-direction: column !important;\n            gap: 1.5em !important;\n        }\n        .dlw-split-image {\n            flex: auto !important;\n            width: 100% !important;\n            max-width: 100% !important;\n        }\n    }\n<\/style>\n\n<!-- ==========================================\n     CONTENIDO UNIFICADO Y BLINDADO\n     ========================================== -->\n<div class=\"dlw-prod-wrapper\">\n\n    <!-- Descripci\u00f3n Corta (Lead Box) -->\n    <div class=\"dlw-lead-box\">\n        <p>High-resolution NGS-based HLA typing system that supports multiple sequencing platforms and offers maximum workflow flexibility.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <p>HLA-SuBiTo NGStype\u00ae is a comprehensive solution for high-resolution HLA typing based on next-generation sequencing (NGS), developed by inno-train Diagnostik. The system allows the simultaneous analysis of up to 11 HLA loci using a long-range PCR approach, compatible with multiple library preparation methods and sequencers.<\/p>\n\n    <p>The technology adapts to both short- and long-read sequencers, including Illumina, Oxford Nanopore Technologies (ONT), and MGI. This gives the user complete freedom to choose the sequencing platform according to their infrastructure or analysis needs.<\/p>\n\n    <!-- Dise\u00f1o Especial: Texto Izquierda \/ Imagen 1 Derecha -->\n    <div class=\"dlw-text-image-split\">\n        <div class=\"dlw-split-text\">\n            <p>The workflow begins with a long-range PCR covering the HLA-A, -B, -C, -DPA1, -DPB1, -DQA1, -DQB1, and DRB1\/3\/4\/5 loci, with amplicons ranging from 3.4 to 7.4 kb. Analysis quality is guaranteed by the use of specific primers and an optimized master mix included in the kit.<\/p>\n            <p>Depending on the chosen workflow, the process can be adapted for laboratories analyzing from a single sample (using ONT&#8217;s MinION) up to hundreds of samples in parallel (using MiSeq, MiniSeq, iSeq, among others). Flexibility is one of its main advantages, along with the system&#8217;s scalability.<\/p>\n        <\/div>\n        <div class=\"dlw-split-image\">\n            <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen1-2.webp\" alt=\"HLA-SuBiTo NGStype PCR Loci\" title=\"\">\n        <\/div>\n    <\/div>\n\n    <p>Library preparation can be performed with validated third-party kits such as NEBNext UltraExpress&#x2122; for Illumina or Rapid Barcoding Kit for ONT, and the launch of a proprietary library preparation kit by inno-train is anticipated.<\/p>\n\n    <p>Data analysis is performed using NGStype\u00ae software, compatible with fastq files from any platform. This software includes optimized algorithms for short- and long-read sequences, allowing automatic haplotype analysis and clear visualization of results.<\/p>\n\n    <!-- Imagen 2 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen2-1.webp\" alt=\"NGStype Software\" class=\"dlw-prod-img\" title=\"\">\n\n    <!-- Aspectos Clave -->\n    <h2>Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>High-resolution HLA typing for 11 loci.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Long-range PCR with specific primers included.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Compatible with Illumina, ONT, and MGI.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Optimized workflows for low or high sample volumes.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Automated data analysis with NGStype\u00ae software.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>High data quality (>Q30) and full coverage per locus.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Validated library preparation for multiple commercial kits.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Fast sequencing possible from 20 minutes (MinION).<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n -->\n    <h2>Ordering Information<\/h2>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Kit name: HLA-SuBiTo NGStype\u00ae \u2013 11 loci<\/span>\n        <span class=\"dlw-list-item\">Contents: reagents for long-range PCR amplification (primers + master mix)<\/span>\n        <span class=\"dlw-list-item\">Compatibility:\n            <div class=\"dlw-sub-list\">\n                <span class=\"dlw-sub-list-item\">Illumina: validated workflows for MiSeq, iSeq, MiniSeq<\/span>\n                <span class=\"dlw-sub-list-item\">ONT: validated workflows with Rapid Barcoding Kit and MinION (FLO-MIN114)<\/span>\n                <span class=\"dlw-sub-list-item\">MGI: compatible (information under development)<\/span>\n            <\/div>\n        <\/span>\n        <span class=\"dlw-list-item\">Software: NGStype\u00ae (automated data analysis)<\/span>\n        <span class=\"dlw-list-item\">No. of samples per kit: 24 samples \u2013 Ref. 002 099 024<\/span>\n        <span class=\"dlw-list-item\">Additional requirements: library preparation kits (NEB or ONT), NGS sequencer<\/span>\n    <\/div>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>High-resolution NGS-based HLA typing system that supports multiple sequencing platforms and offers maximum workflow flexibility. Detailed Description HLA-SuBiTo NGStype\u00ae is a comprehensive solution for high-resolution HLA typing based on next-generation sequencing (NGS), developed by inno-train Diagnostik. The system allows the simultaneous analysis of up to 11 HLA loci using a long-range PCR approach, compatible with [&hellip;]<\/p>\n","protected":false},"featured_media":27157,"template":"","tecnologias":[36],"marcas":[224],"familias":[284,324,321],"class_list":["post-27164","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-inno-train-en","familias-immunology","familias-high-resolution-hla-typing","familias-pre-transplantation"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27164","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27164\/revisions"}],"predecessor-version":[{"id":27166,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27164\/revisions\/27166"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27157"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27164"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27164"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27164"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27164"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}