{"id":27179,"date":"2026-04-13T11:57:45","date_gmt":"2026-04-13T11:57:45","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27179"},"modified":"2026-04-13T12:00:06","modified_gmt":"2026-04-13T12:00:06","slug":"holograft-one","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/holograft-one\/","title":{"rendered":"HoloGRAFT ONE"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n        line-height: 1.6 !important;\n        background-color: transparent !important;\n        padding: 0 !important;\n        max-width: 100% !important;\n        text-align: left !important;\n        display: block !important;\n        font-size: 14px !important; 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}\n        .dlw-check-list { grid-template-columns: 1fr !important; padding: 1.5em !important; }\n        .dlw-prod-img { max-width: 100% !important; }\n    }\n<\/style>\n\n<!-- ==========================================\n     UNIFIED AND SECURED CONTENT\n     ========================================== -->\n<div class=\"dlw-prod-wrapper\">\n\n    <!-- Short Description (Lead Box) -->\n    <div class=\"dlw-lead-box\">\n        <p>Digital PCR assay for the precise quantification of donor-derived cell-free DNA (dd-cfDNA) in transplant patients.<\/p>\n    <\/div>\n\n    <!-- Detailed Description -->\n    <h2>Detailed Description<\/h2>\n\n    <p>HoloGRAFT ONE is a digital PCR (dPCR) assay developed by Werfen for the detection and quantification of donor-derived cell-free DNA (dd-cfDNA) in the plasma of transplant recipient patients. This molecular marker has established itself as a useful tool for non-invasive monitoring of graft status.<\/p>\n\n    <p>During the process of cell damage in the graft\u2014whether due to rejection, infection, or ischemic damage\u2014donor DNA fragments are released into the recipient&#8217;s bloodstream. HoloGRAFT ONE allows for the specific detection and quantification of these fragments using digital PCR, a technology that offers superior sensitivity and precision compared to other molecular methods.<\/p>\n    \n    <!-- Image 1 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen1-3.webp\" alt=\"HoloGRAFT ONE Workflow\" class=\"dlw-prod-img\" title=\"\">\n\n    <p>The methodology requires DNA extraction from plasma, followed by a dPCR reaction with specific probes previously selected based on the genetic differences between donor and recipient. The assay measures the percentage of dd-cfDNA relative to total cell-free DNA, expressed as a percentage.<\/p>\n\n    <p>This value allows the clinician to identify graft injury events early, facilitating therapeutic decisions based on an objective biomarker. HoloGRAFT ONE is applicable to various types of solid organ transplants, such as kidney, liver, heart, or lung, and is easily integrated into post-transplant clinical follow-up workflows.<\/p>\n\n    <!-- Key Features -->\n    <h2>Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Digital PCR (dPCR) assay for dd-cfDNA detection.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Non-invasive biomarker for rejection monitoring.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>High sensitivity and absolute quantification.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Requires only a plasma sample from the recipient.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Expressed as a percentage of donor-derived DNA.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Useful in kidney, liver, heart, and lung transplantation.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Direct clinical interpretation based on reference values.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Compatible with standard dPCR platforms.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Image 2 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/04\/Imagen2-2.webp\" alt=\"HoloGRAFT ONE Data Analysis\" class=\"dlw-prod-img\" title=\"\">\n\n    <!-- Ordering Information -->\n    <h2>Ordering Information<\/h2>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Kit name: HoloGRAFT ONE<\/span>\n        <span class=\"dlw-list-item\">Format: dPCR kit assay (for research use only)<\/span>\n        <span class=\"dlw-list-item\">Sample type: recipient plasma<\/span>\n        <span class=\"dlw-list-item\">Contents: dPCR amplification reagents, specific probes, controls<\/span>\n        <span class=\"dlw-list-item\">Requires: prior donor\/recipient genotyping (selection of informative markers)<\/span>\n        <span class=\"dlw-list-item\">Result: percentage of dd-cfDNA relative to total circulating DNA<\/span>\n        <span class=\"dlw-list-item\">Estimated analysis time: approximately 4 hours<\/span>\n        <span class=\"dlw-list-item\">Compatible instrumentation: validated digital PCR platforms (consult provider)<\/span>\n        <span class=\"dlw-list-item\">Intended use: non-invasive graft monitoring in solid organ transplantation<\/span>\n    <\/div>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Digital PCR assay for the precise quantification of donor-derived cell-free DNA (dd-cfDNA) in transplant patients. Detailed Description HoloGRAFT ONE is a digital PCR (dPCR) assay developed by Werfen for the detection and quantification of donor-derived cell-free DNA (dd-cfDNA) in the plasma of transplant recipient patients. This molecular marker has established itself as a useful tool [&hellip;]<\/p>\n","protected":false},"featured_media":27167,"template":"","tecnologias":[123],"marcas":[253],"familias":[284,319,327],"class_list":["post-27179","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-digital-pcr","marcas-werfen-en","familias-immunology","familias-post-transplantation","familias-solid-organ-dd-cfdna"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27179","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27179\/revisions"}],"predecessor-version":[{"id":27180,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27179\/revisions\/27180"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27167"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27179"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27179"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27179"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27179"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}