{"id":27442,"date":"2026-05-19T14:26:06","date_gmt":"2026-05-19T14:26:06","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27442"},"modified":"2026-05-19T14:29:55","modified_gmt":"2026-05-19T14:29:55","slug":"ad4seq-hbv-solution","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/ad4seq-hbv-solution\/","title":{"rendered":"AD4SEQ HBV Solution"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n        line-height: 1.6 !important;\n        background-color: transparent !important;\n    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{\n        transform: rotate(45deg) !important; \/* Gira el + para que parezca una x *\/\n    }\n\n    .dlw-accordion[open] .dlw-accordion-header {\n        border-bottom: 2px solid var(--dlw-blue-cyan) !important;\n    }\n\n    \/* Ajuste de la tabla dentro del acorde\u00f3n *\/\n    .dlw-accordion-content .dlw-table {\n        margin-bottom: 0 !important;\n        box-shadow: none !important;\n        border-radius: 0 !important;\n        border-top: none !important;\n    }\n\n    \/* Responsive *\/\n    @media (max-width: 768px) {\n        .dlw-lead-box { padding: 1.8em !important; }\n        .dlw-check-list { grid-template-columns: 1fr !important; padding: 1.5em !important; }\n        .dlw-prod-img { max-width: 100% !important; }\n        \n        \/* Tablas Responsive *\/\n        .dlw-table, .dlw-table thead, .dlw-table tbody, .dlw-table th, .dlw-table td, .dlw-table tr { display: block !important; }\n        .dlw-table thead tr { position: absolute !important; top: -9999px !important; left: -9999px !important; }\n        .dlw-table tr { border-bottom: 2px solid var(--dlw-blue-cyan) !important; margin-bottom: 15px !important; }\n        .dlw-table td { border: none !important; position: relative !important; padding-left: 45% !important; }\n        .dlw-table td::before { position: absolute !important; top: 15px !important; left: 15px !important; width: 40% !important; white-space: nowrap !important; font-weight: bold !important; color: var(--dlw-blue-dark) !important; font-size: 0.9em !important; text-transform: uppercase !important; }\n        .dlw-table td:nth-of-type(1)::before { content: \"Reference\" !important; }\n        .dlw-table td:nth-of-type(2)::before { content: \"Description\" !important; }\n    }\n<\/style>\n\n<!-- ==========================================\n     CONTENIDO UNIFICADO Y BLINDADO\n     ========================================== -->\n<div class=\"dlw-prod-wrapper\">\n\n    <!-- Descripci\u00f3n Corta (Lead Box) -->\n    <div class=\"dlw-lead-box\">\n        <p>Qualitative in vitro kit for the determination of the hepatitis B virus (HBV) genotype and the identification of drug resistance mutations through PCR amplification of two viral regions followed by NGS sequencing. It is intended for HBV-positive plasma or serum samples.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <h3>Operating Principle<\/h3>\n    <p>The kit is based on PCR amplification of two regions of the HBV viral DNA with a key role in genotyping and resistance development: Reverse Transcriptase (RT) and preCore (pC), followed by next-generation sequencing (NGS).<\/p>\n    \n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen1-eng-4.webp\" alt=\"HBV Genotyping\" class=\"dlw-prod-img\" title=\"\">\n\n    <p>The amplified regions cover RT: codons 1 &#8211; end and preCore: codons 1 &#8211; end.<\/p>\n    <p>After sequencing, the data can be analyzed with dedicated software that queries reference databases to return a report with the viral genotype, identified mutations, and associated resistances.<\/p>\n    <p>The flowchart on page 3 of the brochure summarizes the analytical process: viral genomic DNA extraction, Target PCR amplification, quality control, AMPure XP purification, TAG PCR, Index PCR, library normalization, NGS sequencing, and data analysis.<\/p>\n\n    <h3>Clinical Applications<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The product is aimed at determining the viral genotype and identifying HBV drug resistance mutations in positive samples.<\/span>\n        <span class=\"dlw-list-item\">The background document indicates that NGS sequencing is an appropriate technique for HBV genotyping and resistance mutation screening.<\/span>\n    <\/div>\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Analyzes in a single workflow two relevant regions of the viral genome associated with genotyping and therapeutic resistance: RT and preCore.<\/span>\n        <span class=\"dlw-list-item\">Allows combining amplification, sequencing, and bioinformatics analysis in a standardized procedure.<\/span>\n        <span class=\"dlw-list-item\">Can be sequenced on various Illumina platforms, facilitating its integration into different laboratory environments.<\/span>\n        <span class=\"dlw-list-item\">The brochure indicates sequencing library preparation in approximately 6 hours and a complete workflow of up to 24 hours, according to the scheme on page 3.<\/span>\n    <\/div>\n\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen2-eng-3.webp\" alt=\"NGS Workflow\" class=\"dlw-prod-img\" title=\"\">\n\n    <h3>Key Results or Indicators<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Recommended samples: HBV-positive plasma and serum.<\/span>\n        <span class=\"dlw-list-item\">LOD: \u2265 500 IU\/ml. For samples with lower viremia, contact Customer Service.<\/span>\n        <span class=\"dlw-list-item\">To obtain good coverage of all amplified regions, approximately 40,000 reads per sample are recommended.<\/span>\n        <span class=\"dlw-list-item\">The document indicates that it is possible to identify mutations at 1% with 3000x coverage.<\/span>\n        <span class=\"dlw-list-item\">In the quality control of Target PCR products, bands of approximately 1200 bp are expected for Amp Mix A and 1000 bp for Amp Mix B.<\/span>\n        <span class=\"dlw-list-item\">In the quality control of Index PCR products, fragments of approximately 375 bp are expected.<\/span>\n        <span class=\"dlw-list-item\">The recommended software automatically sets a minimum coverage threshold of 1000 reads; codons with lower coverage can also be evaluated, with a configurable minimum of 30 reads.<\/span>\n    <\/div>\n\n    <h3>Technology Used<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">PCR amplification of genomic viral DNA and NGS sequencing.<\/span>\n        <span class=\"dlw-list-item\">AMPure XP purification, sample indexing, and sequencing on Illumina MiSeq, MiniSeq, iSeq 100, or MiSeq i100 platforms.<\/span>\n        <span class=\"dlw-list-item\">Analysis of FASTQ files is recommended with SmartVir, developed by SmartSeq s.r.l., or equivalent software capable of genotyping HBV and identifying mutations in the sequenced regions.<\/span>\n    <\/div>\n\n    <h3>Intended Audience \/ User<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">For use in HBV-positive plasma or serum samples.<\/span>\n        <span class=\"dlw-list-item\">RUO kit, for use by qualified and adequately trained personnel.<\/span>\n    <\/div>\n\n    <h3>Considerations or Limitations<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The kit is exclusively for manual use; any automation must be validated by the user.<\/span>\n        <span class=\"dlw-list-item\">Does not include reagents for viral DNA extraction.<\/span>\n        <span class=\"dlw-list-item\">Requires additional reagents and equipment, including AMPure XP, Illumina sequencing kits, PhiX, and corresponding laboratory instruments.<\/span>\n        <span class=\"dlw-list-item\">Reagents must be stored between -25 \u00b0C and -15 \u00b0C, allow a maximum of 4 freeze\/thaw cycles, and must be kept between +2 \u00b0C and +8 \u00b0C during use.<\/span>\n        <span class=\"dlw-list-item\">For good extraction and NGS analysis performance, it is recommended to use samples stored below -65 \u00b0C and without freeze\/thaw cycles.<\/span>\n        <span class=\"dlw-list-item\">The presence of EDTA above 2 mM can inhibit subsequent amplification.<\/span>\n        <span class=\"dlw-list-item\">Target PCR bands may not be clearly visible, especially in samples with very low viremia.<\/span>\n    <\/div>\n\n    <h3>Other Relevant Technical Information<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The analytical protocol comprises 10 stages: DNA extraction\/handling, Target PCR, purification, TAG PCR, purification, Index PCR, purification, normalization and pooling, sequencing, and data analysis.<\/span>\n        <span class=\"dlw-list-item\">Depending on the platform and sequencing kit, the system supports from up to 30 samples in MiSeq Nano Kit v2 to up to 96 samples in MiSeq Micro Kit v2, MiniSeq Mid Output, iSeq 100 i1, or MiSeq i100 Series 5M.<\/span>\n        <span class=\"dlw-list-item\">For samples with low coverage, the document indicates that if a minimum coverage of 100X per region is reached, re-sequencing is not necessary; if some codons fall below 100 reads, whether to repeat sequencing or issue the report should be evaluated.<\/span>\n    <\/div>\n\n    <!-- Aspectos Clave -->\n    <h2>Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>HBV genotyping and identification of drug resistance mutations.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Amplification of two target regions: Reverse Transcriptase and preCore.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Use in HBV-positive plasma\/serum samples.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>LOD \u2265 500 IU\/ml.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Compatible with Illumina MiSeq, MiniSeq, iSeq 100, and MiSeq i100.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Library preparation in approximately 6 hours and a total workflow of up to 24 hours, according to the brochure&#8217;s scheme.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Bioinformatics analysis with recommended SmartVir software.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>RUO presentation for 30 reactions.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones) -->\n    <h2>Ordering Information<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Format and Reference<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">AD-004.030<\/td>\n                        <td width=\"70%\">Kit for 30 reactions<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Kit Contents<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <div class=\"dlw-list\" style=\"margin-bottom: 0 !important;\">\n                <span class=\"dlw-list-item\"><strong>Amp Mix A (red insert):<\/strong> 1 x 36 \u00b5l. Oligonucleotide mix for amplification of the HBV Reverse Transcriptase region.<\/span>\n                <span class=\"dlw-list-item\"><strong>Amp Mix B (white insert):<\/strong> 1 x 36 \u00b5l. Oligonucleotide mix for amplification of the HBV preCore region.<\/span>\n                <span class=\"dlw-list-item\"><strong>Target Mix (orange cap):<\/strong> 1 x 720 \u00b5l. Solution with the enzyme mix for Target PCR.<\/span>\n                <span class=\"dlw-list-item\"><strong>Tag Mix (black insert):<\/strong> 1 x 33 \u00b5l. Solution with the enzyme mix for viral DNA digestion.<\/span>\n                <span class=\"dlw-list-item\"><strong>2X Reaction Buffer (purple insert):<\/strong> 1 x 300 \u00b5l. Buffer for the digestion reaction.<\/span>\n                <span class=\"dlw-list-item\"><strong>STB (blue cap):<\/strong> 1 x 1000 \u00b5l. Buffer for the digestion reaction.<\/span>\n                <span class=\"dlw-list-item\"><strong>Enzyme Mix 6 (yellow cap):<\/strong> 1 x 360 \u00b5l. Solution with the enzyme mix for Index PCR.<\/span>\n                <span class=\"dlw-list-item\"><strong>iPLATE_i97_i128_HBV:<\/strong> 1 x 32 indices for indexing.<\/span>\n            <\/div>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Required Material Not Included<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <p>Viral DNA extraction kit, AMPure XP, compatible Illumina sequencing kit, PhiX Control v3, molecular biology grade water, ethanol, and specific laboratory equipment, including thermal cycler and compatible Illumina sequencer.<\/p>\n        <\/div>\n    <\/details>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Qualitative in vitro kit for the determination of the hepatitis B virus (HBV) genotype and the identification of drug resistance mutations through PCR amplification of two viral regions followed by NGS sequencing. It is intended for HBV-positive plasma or serum samples. Detailed Description Operating Principle The kit is based on PCR amplification of two regions [&hellip;]<\/p>\n","protected":false},"featured_media":27440,"template":"","tecnologias":[36],"marcas":[218],"familias":[328,335],"class_list":["post-27442","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-arrow-diagnostics-en","familias-microbiology","familias-virus-en"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27442","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27442\/revisions"}],"predecessor-version":[{"id":27443,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27442\/revisions\/27443"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27440"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27442"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27442"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27442"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27442"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}