{"id":27467,"date":"2026-05-20T09:31:22","date_gmt":"2026-05-20T09:31:22","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27467"},"modified":"2026-05-20T09:50:25","modified_gmt":"2026-05-20T09:50:25","slug":"ad4seq-hcv-solution-hcv-solution","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/ad4seq-hcv-solution-hcv-solution\/","title":{"rendered":"AD4SEQ HCV Solution (HCV Solution)"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n        line-height: 1.6 !important;\n        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padding: 1.5em !important; }\n        .dlw-prod-img { max-width: 100% !important; }\n        \n        .dlw-table, .dlw-table thead, .dlw-table tbody, .dlw-table th, .dlw-table td, .dlw-table tr { display: block !important; }\n        .dlw-table thead tr { position: absolute !important; top: -9999px !important; left: -9999px !important; }\n        .dlw-table tr { border-bottom: 2px solid var(--dlw-blue-cyan) !important; margin-bottom: 15px !important; }\n        .dlw-table td { border: none !important; position: relative !important; padding-left: 45% !important; }\n        .dlw-table td::before { position: absolute !important; top: 15px !important; left: 15px !important; width: 40% !important; white-space: nowrap !important; font-weight: bold !important; color: var(--dlw-blue-dark) !important; font-size: 0.9em !important; text-transform: uppercase !important; }\n        .dlw-table td:nth-of-type(1)::before { content: \"Data\" !important; }\n        .dlw-table td:nth-of-type(2)::before { content: \"Value\" !important; }\n    }\n<\/style>\n\n<!-- ==========================================\n     CONTENIDO UNIFICADO Y BLINDADO\n     ========================================== -->\n<div class=\"dlw-prod-wrapper\">\n\n    <!-- Descripci\u00f3n Corta (Lead Box) -->\n    <div class=\"dlw-lead-box\">\n        <p>Standardized kit for the detection of Hepatitis C Virus (HCV) genotype and the determination of drug resistance through next-generation sequencing (NGS) of the viral genome. It is exclusively intended for HCV-positive plasma or serum samples and is complemented by dedicated software for data visualization and computational analysis.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>3.- Detailed Description<\/h2>\n\n    <h3>Operating principle<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The kit is based on an RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) strategy followed by NGS sequencing of the HCV viral genome from a biological sample. It uses pangenotypic and genotype-specific probes at different stages of the protocol to simultaneously amplify the main viral genotypes.<\/span>\n        <span class=\"dlw-list-item\">The WG amplification allows sequencing approximately 90% of the HCV genome, from the 5&#8217;UTR region to codon 346 of NS5B. The additional amplification of the MIDI region extends the coverage up to approximately 97% of the genome, from 5&#8217;UTR to 3&#8217;UTR.<\/span>\n    <\/div>\n\n    <!-- Imagen 1 con Pop-up -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen1-eng-5.webp\" alt=\"HCV amplification scheme\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Following sequencing, FASTQ files can be analyzed with dedicated software, with the express recommendation of SmartVir (developed by SmartSeq s.r.l.), to indicate the HCV genotype and identify mutated codons in the target regions.<\/span>\n    <\/div>\n\n    <h3>Clinical applications<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Its utility lies in the genotypic characterization of HCV and the evaluation of drug-associated resistance. It highlights the importance of defining the viral genotype before starting therapy, redefining it in patients with treatment failure to direct-acting antivirals (DAAs), and identifying associated pharmacological resistance.<\/span>\n    <\/div>\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Integrates viral genome amplification, NGS sequencing, and downstream bioinformatics analysis into a single workflow.<\/span>\n        <span class=\"dlw-list-item\">Allows the identification of genotypes 1, 2, 3, 4, 5, 6, and 7, as well as subtypes, along with the evaluation of drug-associated resistance in the sequenced regions.<\/span>\n        <span class=\"dlw-list-item\">It is compatible with multiple Illumina platforms, facilitating its integration into different sequencing environments.<\/span>\n    <\/div>\n\n    <h3>Key results or indicators<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Coverage of up to 97% of the HCV genome with the complete WG + MIDI strategy.<\/span>\n        <span class=\"dlw-list-item\">Recommended LOD: \u2265 1000 IU\/ml in plasma or serum. For samples with lower viremia, contact technical support.<\/span>\n        <span class=\"dlw-list-item\">To obtain good coverage of all amplified regions, approximately 200,000 reads per sample are recommended. The document indicates that it is possible to identify mutations at 1% with 3000x coverage.<\/span>\n        <span class=\"dlw-list-item\">The SmartVir software automatically sets a minimum threshold of 1000 reads; it also allows evaluating codons with lower coverage, with a configurable minimum of 30 reads.<\/span>\n    <\/div>\n\n    <h3>Technology used<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">RT-PCR for reverse transcription and amplification, followed by Whole Genome Sequencing (WGS) using NGS.<\/span>\n        <span class=\"dlw-list-item\">Purification with AMPure XP, sample indexing, and sequencing on Illumina MiSeq, MiniSeq, iSeq 100, or MiSeq i100.<\/span>\n    <\/div>\n\n    <h3>Intended audience\/user<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Exclusive use in HCV-positive plasma or serum samples.<\/span>\n        <span class=\"dlw-list-item\">RUO kit, intended to be used by qualified and adequately trained personnel.<\/span>\n    <\/div>\n\n    <h3>Considerations or limitations<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">It is an exclusively manual use kit; any automation must be validated by the user.<\/span>\n        <span class=\"dlw-list-item\">It does not include reagents for viral RNA extraction and requires additional reagents, consumables, and equipment to complete the workflow.<\/span>\n        <span class=\"dlw-list-item\">Reagents must be stored between -25 \u00b0C and -15 \u00b0C; they allow a maximum of 4 freeze\/thaw cycles and, during use, must be kept on ice or between +2 \u00b0C and +8 \u00b0C.<\/span>\n        <span class=\"dlw-list-item\">The reliability of the results may be affected by sample collection, transport, storage, and processing. The extracted RNA should be stored below -65 \u00b0C, and avoiding freeze\/thaw cycles is recommended. Furthermore, EDTA concentrations > 2 mM can inhibit amplification.<\/span>\n        <span class=\"dlw-list-item\">In samples with a viremia lower than 500,000 IU\/ml, the WG band may not be visualized in the quality control, although the protocol recommends continuing with the subsequent phases.<\/span>\n    <\/div>\n\n    <h3>Other relevant technical information<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The analytical protocol comprises 11 stages: RNA handling\/extraction, RT-PCR, Target PCR, intermediate purifications, TAG PCR, Index PCR, normalization and pool preparation, sequencing, and data analysis.<\/span>\n    <\/div>\n\n    <!-- Imagen 2 con Pop-up -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen2-eng-4.webp\" alt=\"Sequencing workflow\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Depending on the platform and sequencing reagent used, the system accommodates from up to 10 samples on MiSeq Nano v2 (300 cycles) to 75 samples on MiniSeq Mid Output (300 cycles).<\/span>\n    <\/div>\n\n    <!-- Aspectos Clave -->\n    <h2>4.- Key Features<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>HCV genotyping and drug resistance determination through NGS.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Coverage of up to 97% of the viral genome with WG + MIDI strategy.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Identification of genotypes 1-7 and subtypes.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Exclusive use in HCV-positive plasma\/serum, with recommended LOD \u2265 1000 IU\/ml.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Compatible with Illumina MiSeq, MiniSeq, iSeq 100, and MiSeq i100 platforms.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Data analysis with recommended dedicated SmartVir software.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Manual use RUO presentation for 30 reactions.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones Desplegables) -->\n    <h2>5.- Ordering Information<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Format and reference<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">AD-006.030<\/td>\n                        <td width=\"70%\">Presentation for 30 reactions<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Kit contents<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">Amp Mix RT (green insert): 80 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">dNTPs Solution (orange cap): 40 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">RT Mix (clear insert): 40 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">RT Buffer (5X) (yellow cap): 160 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">DTT Solution (white cap): 40 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">Amp Mix WG (blue insert): 64 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">Amp Mix MIDI (red insert): 400 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">Target Mix HCV (brown cap): 1400 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">TAG Mix (black insert): 33 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">2X Reaction Buffer (purple insert): 300 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">STB (blue cap): 1000 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">Index Mix (black cap): 330 \u00b5l<\/span>\n                <span class=\"dlw-list-item\">iPlate_i129_i160_HCV: 1 x 32 indices for PCR product indexing<\/span>\n            <\/div>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Required material not included<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <p>Reagents for viral RNA extraction, AMPure XP, compatible Illumina sequencing kits, PhiX Control v3, molecular biology grade water, ethanol, and specific laboratory equipment, including thermal cycler and compatible Illumina sequencer.<\/p>\n        <\/div>\n    <\/details>\n\n    <!-- Estructura del Modal (Lightbox) -->\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n    <\/div>\n\n<\/div>\n\n<!-- Script del Modal (Vainilla JS, sin dependencias) -->\n<script>\n    function openDlwModal(src) {\n        var modal = document.getElementById('dlw-image-modal');\n        var modalImg = document.getElementById('dlw-modal-img-target');\n        modalImg.src 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the determination of drug resistance through next-generation sequencing (NGS) of the viral genome. 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