{"id":27478,"date":"2026-05-20T10:02:29","date_gmt":"2026-05-20T10:02:29","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27478"},"modified":"2026-05-20T11:11:50","modified_gmt":"2026-05-20T11:11:50","slug":"ad4seq-hiv-1-whole-genome","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/ad4seq-hiv-1-whole-genome\/","title":{"rendered":"AD4SEQ HIV-1 Whole Genome"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded and Scaled to 14px) -->\n<style>\n    \/* Corporate fonts import *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Isolated Main Container *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n        line-height: 1.6 !important;\n        background-color: transparent !important;\n        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Following amplification, Next Generation Sequencing (NGS) allows for viral genotyping and evaluation of mutations present in the viral genome.<\/p>\n    <\/div>\n\n    <!-- Detailed Description -->\n    <h2>Detailed Description<\/h2>\n\n    <h3>Principle of operation<\/h3>\n    <p>The AD4SEQ HIV-1 Whole Genome kit is based on RT-PCR amplification of the complete HIV-1 viral RNA obtained from human plasma or serum samples. The amplified product is subsequently processed for NGS sequencing, allowing viral genotyping and identification of mutations present in the viral genome, including those located in reference target regions.<\/p>\n    <p>The described analytical procedure includes a workflow consisting of: RNA handling and extraction, One Step RT-PCR Target, purification of RT-PCR products, PCR TAG, PCR TAG purification, PCR Index, PCR Index purification, sample pool normalization and preparation, sequencing on Illumina\u00ae platforms, and data analysis.<\/p>\n    \n    <!-- Image 1 with Pop-up (Lightbox) -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen1-eng-6.webp\" alt=\"AD4SEQ HIV-1 Whole Genome workflow\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n    <h3>Clinical applications \/ intended research use<\/h3>\n    <p>The product is indicated for Research Use Only (R.U.O.) and can be used on HIV-1 positive plasma or serum samples. Its purpose is to investigate the complete scenario of viral mutations present in HIV-1 positive samples by viral genotyping and evaluation of mutations in the viral genome.<\/p>\n    <p>The documentation highlights the relevance of evaluating HIV-1 genome mutations for the success of antiretroviral treatments. In addition to classic regions such as protease, reverse transcriptase, integrase, and V3Loop, the kit allows investigation of additional viral regions such as gag, vif, nef, and regions adjacent to integrase.<\/p>\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Allows complete HIV-1 genome analysis using an RT-PCR and NGS-based strategy.<\/span>\n        <span class=\"dlw-list-item\">Facilitates viral genotyping and identification of mutations present in regions of interest within the genome.<\/span>\n        <span class=\"dlw-list-item\">Expands research beyond protease, reverse transcriptase, integrase, and V3Loop, including regions like gag, vif, nef, and integrase-adjacent regions.<\/span>\n        <span class=\"dlw-list-item\">The product&#8217;s technical brochure indicates performance features such as sequencing library preparation in 8 hours, recommended input \u22651000 cp\/ml, genotyping of HIV-1 group M subtypes including recombinants, drug resistance detection, HIV-1 tropism analysis in gp120, and analysis using dedicated software.<\/span>\n    <\/div>\n\n    <h3>Results or key indicators<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">It is possible to identify mutations down to 1% with 3000x coverage.<\/span>\n        <span class=\"dlw-list-item\">To obtain good coverage of all amplified viral regions, approximately 120,000 reads per sample are recommended.<\/span>\n        <span class=\"dlw-list-item\">Data analysis is performed from fastq files obtained after NGS sequencing. The use of SmartVir software, produced by SmartSeq s.r.l., or similar programs capable of complete HIV-1 genome genotyping and mutated codon identification is recommended.<\/span>\n        <span class=\"dlw-list-item\">The result of the suggested analysis methods is expressed as a relative abundance value of the mutations present in the sample.<\/span>\n        <span class=\"dlw-list-item\">SmartVir establishes a minimum coverage threshold per codon of 100 reads; codons with lower coverage can also be evaluated, down to a configurable minimum of 30 reads, considering mutations with a minimum coverage of 30 reads as reliable.<\/span>\n    <\/div>\n\n    <h3>Technology used<\/h3>\n    <p>The kit combines viral RNA amplification by One Step RT-PCR, subsequent preparation via PCR TAG and PCR Index, purification with AMPure\u00ae XP magnetic beads, library normalization and pooling, and sequencing using Illumina\u00ae platforms.<\/p>\n    <p>The Illumina\u00ae platforms indicated in the documentation are MiSeq&#x2122;, MiniSeq&#x2122;, iSeq&#x2122; 100, and MiSeq&#x2122; i100, with different reagent kits, maximum number of samples, and sequencing times depending on the platform.<\/p>\n\n    <h3>Intended audience\/user<\/h3>\n    <p>The R.U.O. kit must be used by qualified and properly trained personnel to avoid inadequate performance resulting from errors in sample or reagent dispensing, or incorrect sequencer configuration.<\/p>\n\n    <h3>Considerations or limitations<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Product for Research Use Only (R.U.O.).<\/span>\n        <span class=\"dlw-list-item\">The kit can be used on HIV-1 positive plasma or serum samples.<\/span>\n        <span class=\"dlw-list-item\">It is recommended to work with samples having a viral load \u22651000 cp\/ml; for lower viral loads, the documentation refers to specific instructions and consultation with the local specialist.<\/span>\n        <span class=\"dlw-list-item\">The kit does not contain reagents for viral genomic RNA extraction; extraction must be performed using methods recommended or validated by the user.<\/span>\n        <span class=\"dlw-list-item\">The presence of EDTA at concentrations greater than 2 mM may inhibit the subsequent amplification reaction.<\/span>\n        <span class=\"dlw-list-item\">Reliability of results may be influenced by sample collection, transport, storage, and processing.<\/span>\n        <span class=\"dlw-list-item\">The kit is intended exclusively for manual use; any automation must be validated by the user.<\/span>\n    <\/div>\n\n    <!-- Key Aspects -->\n    <h2>Key Aspects<\/h2>\n    \n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Qualitative kit for complete HIV-1 genome analysis.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Based on RT-PCR followed by NGS sequencing.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Allows viral genotyping and evaluation of HIV-1 genome mutations.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Applicable to HIV-1 positive plasma or serum samples.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Investigation of relevant viral regions such as gag, vif, nef, and integrase-adjacent regions.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Compatible with Illumina\u00ae MiSeq&#x2122;, MiniSeq&#x2122;, iSeq&#x2122; 100, and MiSeq&#x2122; i100 platforms.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Possibility to identify mutations down to 1% with 3000x coverage.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Recommendation of approximately 120,000 reads per sample for good coverage.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Data analysis recommended using SmartVir or equivalent software.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>R.U.O. product, intended exclusively for research.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Presentation Details (Dropdown Accordions) -->\n    <h2>Presentation Details<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Format and reference<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">Product<\/td>\n                        <td width=\"70%\">HIV-1 Whole Genome<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Line<\/td>\n                        <td>AD4SEQ<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Reference<\/td>\n                        <td>AD-012.030<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Presentation<\/td>\n                        <td>30 reactions<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">Use<\/td>\n                        <td>R.U.O. \/ Research Use Only<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Kit contents<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">WG Px Mix 1: 40 \u00b5l, oligonucleotide mix for viral genome amplification.<\/span>\n                <span class=\"dlw-list-item\">WG Px Mix 2: 40 \u00b5l, oligonucleotide mix for viral genome amplification.<\/span>\n                <span class=\"dlw-list-item\">WG Px Mix 3: 40 \u00b5l, oligonucleotide mix for viral genome amplification.<\/span>\n                <span class=\"dlw-list-item\">WG Px Mix 4: 40 \u00b5l, oligonucleotide mix for viral genome amplification.<\/span>\n                <span class=\"dlw-list-item\">WG RT Mix: 80 \u00b5l, enzyme mix solution for One Step RT-PCR.<\/span>\n                <span class=\"dlw-list-item\">WG 2X Master Mix: 1000 \u00b5l, buffer solution for One Step RT-PCR.<\/span>\n                <span class=\"dlw-list-item\">TAG Mix: 33 \u00b5l, enzyme mix solution for PCR TAG.<\/span>\n                <span class=\"dlw-list-item\">2X Reaction Buffer: 300 \u00b5l, buffer solution for PCR TAG.<\/span>\n                <span class=\"dlw-list-item\">STB: 1000 \u00b5l, post PCR TAG buffer solution.<\/span>\n                <span class=\"dlw-list-item\">Index Mix: 330 \u00b5l, enzyme mix solution for PCR Index.<\/span>\n                <span class=\"dlw-list-item\">iPLATE_i193_i224_WGS: 32 indexes, oligonucleotide mix for indexing PCR amplified samples.<\/span>\n            <\/div>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Storage and stability<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">Store all reagents between -25 \u00b0C and -15 \u00b0C.<\/span>\n                <span class=\"dlw-list-item\">Components are ready to use and stable until the indicated expiration date if stored according to recommendations.<\/span>\n                <span class=\"dlw-list-item\">Reagents can undergo a maximum of 4 freeze\/thaw cycles without compromising their functionality.<\/span>\n                <span class=\"dlw-list-item\">During use, reagents must be kept on ice or in refrigerated racks between +2 \u00b0C and +8 \u00b0C.<\/span>\n                <span class=\"dlw-list-item\">Reagents are not reusable, and intermediate mixture residues should not be stored.<\/span>\n            <\/div>\n        <\/div>\n    <\/details>\n\n    <!-- Modal Structure (Lightbox) -->\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n    <\/div>\n\n<\/div>\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n<script>\n    function openDlwModal(src) {\n        var modal = document.getElementById('dlw-image-modal');\n        var modalImg = document.getElementById('dlw-modal-img-target');\n        modalImg.src = src;\n        modal.classList.add('dlw-active');\n        document.body.style.overflow = 'hidden'; \/\/ Locks background scroll\n    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Following amplification, Next Generation Sequencing (NGS) allows for viral genotyping and evaluation of mutations present in the viral genome. Detailed Description Principle of operation The AD4SEQ HIV-1 Whole Genome kit is based on RT-PCR [&hellip;]<\/p>\n","protected":false},"featured_media":27476,"template":"","tecnologias":[36],"marcas":[218],"familias":[328,335],"class_list":["post-27478","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-arrow-diagnostics-en","familias-microbiology","familias-virus-en"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":3,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27478\/revisions"}],"predecessor-version":[{"id":27482,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27478\/revisions\/27482"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27476"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27478"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27478"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27478"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}