{"id":27504,"date":"2026-05-21T07:53:55","date_gmt":"2026-05-21T07:53:55","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27504"},"modified":"2026-05-21T08:14:50","modified_gmt":"2026-05-21T08:14:50","slug":"ad4seq-microbiota-solution-a-microbiota-solution-b","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/ad4seq-microbiota-solution-a-microbiota-solution-b\/","title":{"rendered":"AD4SEQ Microbiota Solution A \/ Microbiota Solution B"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 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   \/* Responsive *\/\n    @media (max-width: 768px) {\n        .dlw-lead-box { padding: 1.8em !important; }\n        .dlw-check-list { grid-template-columns: 1fr !important; padding: 1.5em !important; }\n        .dlw-prod-img { max-width: 100% !important; }\n\n        \/* Tablas Responsive *\/\n        .dlw-table, .dlw-table thead, .dlw-table tbody, .dlw-table th, .dlw-table td, .dlw-table tr { display: block !important; }\n        .dlw-table thead tr { position: absolute !important; top: -9999px !important; left: -9999px !important; }\n        .dlw-table tr { border-bottom: 2px solid var(--dlw-blue-cyan) !important; margin-bottom: 15px !important; }\n        .dlw-table td { border: none !important; position: relative !important; padding-left: 45% !important; }\n        .dlw-table td::before { position: absolute !important; top: 15px !important; left: 15px !important; width: 40% !important; white-space: nowrap !important; font-weight: bold !important; color: var(--dlw-blue-dark) !important; font-size: 0.9em !important; text-transform: uppercase !important; }\n        .dlw-table td:nth-of-type(1)::before { content: \"Reference\" !important; }\n        .dlw-table td:nth-of-type(2)::before { content: \"Description\" !important; }\n    }\n<\/style>\n\n<!-- ==========================================\n     CONTENIDO UNIFICADO Y BLINDADO\n     ========================================== -->\n<div class=\"dlw-prod-wrapper\">\n\n    <!-- Descripci\u00f3n Corta (Lead Box) -->\n    <div class=\"dlw-lead-box\">\n        <p>In vitro diagnostic kit based on PCR and Next-Generation Sequencing (NGS) for the qualitative evaluation of the microbiome through the analysis of hypervariable regions of the bacterial 16S rDNA gene. It is designed for human gut microbiota and other biological samples, and can be used as a diagnostic aid in the clinical context of fecal microbiota transplantation, both in patient monitoring and donor screening.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <h3>Operating Principle<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The system is based on PCR amplification of the hypervariable regions of the bacterial 16S rDNA gene using degenerate primers, followed by NGS sequencing, allowing the identification of bacterial populations present in the sample without the need for culture.<\/span>\n        <span class=\"dlw-list-item\">The analyzed region depends on the kit:\n            <div class=\"dlw-sub-list\">\n                <span class=\"dlw-sub-list-item\">Microbiota Solution A (AD-001.024): V1-V2-V3 regions<\/span>\n                <span class=\"dlw-sub-list-item\">Microbiota Solution B (AD-002.024): V3-V4-V6 regions<\/span>\n            <\/div>\n        <\/span>\n    <\/div>\n\n    <!-- Imagen 1 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen1-eng-7.webp\" alt=\"Operating Principle Microbiota Solution\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">After sequencing, data can be automatically analyzed using software to identify the species present in an individual sample or in comparative samples selected by the user.<\/span>\n    <\/div>\n\n    <h3>Clinical Applications<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Qualitative evaluation of the human gut microbiota and other biological samples, including vaginal swab, endometrial catheter, sputum, skin swab, saliva, feces, and rectal swab.<\/span>\n        <span class=\"dlw-list-item\">It can be used as a diagnostic aid in the clinical context of fecal microbiota transplantation, both for post-treatment monitoring and donor screening.<\/span>\n    <\/div>\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Allows the identification of bacterial populations avoiding the culture phase and, potentially, without the selective bias introduced by laboratory culture conditions.<\/span>\n        <span class=\"dlw-list-item\">Provides a standardized approach for the clinical analysis of the microbiota, useful for identifying bacterial profiles already associated with specific pathologies and for detecting new bacterial profiles.<\/span>\n        <span class=\"dlw-list-item\">Library preparation in 8 hours and a total workflow of up to 24 hours.<\/span>\n    <\/div>\n\n    <h3>Results or Key Indicators<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Analytical specificity: in 10 bacterial DNA extracts from 10 soil samples, none of the evaluated target species were detected; the document reports NPA (Negative Percent Agreement) = 100%.<\/span>\n        <span class=\"dlw-list-item\">Analytical sensitivity: ability to identify bacterial species present in the sample at a percentage \u22650.16%, under the coverage conditions indicated in the internal study.<\/span>\n        <span class=\"dlw-list-item\">Reproducibility: acceptance criteria were met with intra-run CV &lt; 10%, inter-run CV &lt; 15%, and inter-lot CV &lt; 15%; the document concludes that the results are highly replicable.<\/span>\n        <span class=\"dlw-list-item\">Clinical performance: in a comparison with the standard Illumina procedure for human gut microbiota, 7 stool samples were analyzed in triplicate (total 21 samples), concluding that Microbiota Solution A and Microbiota Solution B are comparable to the data obtained with the protocol suggested by Illumina.<\/span>\n        <span class=\"dlw-list-item\">In the Target PCR quality control, amplicons of approximately 650 bp are expected for Solution A and 840 bp for Solution B.<\/span>\n        <span class=\"dlw-list-item\">For library normalization, the document uses average lengths of 640 bp as a reference for the kit with Amp Mix V1-V3 and 900 bp for the kit with Amp Mix V3-V6.<\/span>\n    <\/div>\n\n    <h3>Technology Used<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">DNA amplification by PCR of hypervariable regions of the 16S rDNA and NGS sequencing.<\/span>\n        <span class=\"dlw-list-item\">The analytical protocol comprises 8 phases: DNA extraction and quantification, Target PCR, Target product purification, Index PCR, Index product purification, normalization and pool preparation, sequencing, and data analysis.<\/span>\n    <\/div>\n\n    <!-- Imagen 2 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen-2-eng.webp\" alt=\"Microbiota Solution Workflow\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Sequencing is performed on the Illumina MiSeq\u00ae platform with the MiSeq Reagent Nano Kit v2 (500-cycles). For library loading, the document recommends 7 pM for the AD-001.024 kit and 5 pM for the AD-002.024 kit, also adjusting the sample with 10% PhiX Control v3.<\/span>\n        <span class=\"dlw-list-item\">Data analysis is recommended with MicrobAT software (SmartSeq s.r.l.) or similar software capable of assigning most reads up to the species taxonomic level and returning descriptive statistics such as rarefaction curve, alpha-diversity, dysbiosis index, and enterotype in fecal samples.<\/span>\n    <\/div>\n\n    <h3>Target Audience\/User<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Product for professional use.<\/span>\n        <span class=\"dlw-list-item\">The document indicates that it must be used by qualified and properly trained personnel.<\/span>\n    <\/div>\n\n    <h3>Considerations or Limitations<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The kit is for manual use only; any automation must be validated by the user.<\/span>\n        <span class=\"dlw-list-item\">The globally recommended samples are: feces, rectal swab, vaginal swab, endometrial catheter, skin swab, sputum, and saliva. For correct identification down to the species level, the document recommends:\n            <div class=\"dlw-sub-list\">\n                <span class=\"dlw-sub-list-item\">Solution A: skin sample, sputum, and saliva<\/span>\n                <span class=\"dlw-sub-list-item\">Solution B: feces, rectal swab, vaginal swab, and endometrial catheter<\/span>\n            <\/div>\n        <\/span>\n        <span class=\"dlw-list-item\">If Microbiota Solution A is used with vaginal swab or endometrial catheter samples, it will not be possible to identify the Gardnerella spp. genus at the species level.<\/span>\n        <span class=\"dlw-list-item\">Microbiota analysis can potentially be performed on other types of biological samples, but in that case, both the use of the kit and the DNA extraction method must be validated by the user.<\/span>\n        <span class=\"dlw-list-item\">For subsequent reactions, 5 \u00b5l of a 1 ng\/\u00b5l DNA solution is required. If the concentration is lower, the sample can be used as is, verifying that the DNA is eluted in molecular grade water or in an EDTA-free buffer, as concentrations above 2 mM can inhibit amplification.<\/span>\n        <span class=\"dlw-list-item\">The analysis results express the relative abundance of the detected species and cannot be interpreted as an absolute quantity. The document recommends always interpreting the microbial profile together with the clinical history and other available diagnostic data.<\/span>\n    <\/div>\n\n    <!-- Aspectos Clave -->\n    <h2>Key Aspects<\/h2>\n\n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Qualitative evaluation of the microbiome via 16S rDNA PCR and NGS sequencing.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Two configurations depending on the target region:\n                <div class=\"dlw-sub-list\" style=\"margin-left: 0.5em !important; font-size: 0.9em !important;\">\n                    <span class=\"dlw-sub-list-item\" style=\"color: inherit !important;\">Solution A: V1-V2-V3<\/span>\n                    <span class=\"dlw-sub-list-item\" style=\"color: inherit !important;\">Solution B: V3-V4-V6<\/span>\n                <\/div>\n            <\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Indicated for gut microbiota and other recommended biological matrices.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Use as a diagnostic aid in fecal microbiota transplantation: monitoring and donor screening.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>NPA = 100% in the analytical specificity evaluation described in the document.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Highly reproducible results under the CV criteria defined by the manufacturer.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Library preparation in 8 hours and workflow of up to 24 hours, according to the brochure.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Recommended bioinformatic analysis with MicrobAT.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones Desplegables) -->\n    <h2>Presentation Details<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Format<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">Kit<\/td>\n                        <td width=\"70%\">For 24 reactions.<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">References<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">AD-001.024<\/td>\n                        <td width=\"70%\">Microbiota Solution A<\/td>\n                    <\/tr>\n                    <tr>\n                        <td class=\"dlw-ref-code\">AD-002.024<\/td>\n                        <td>Microbiota Solution B<\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Kit contents<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">Enzyme Mix 1 (red cap): 1 x 340 \u00b5l. Enzyme mix for Target PCR.<\/span>\n                <span class=\"dlw-list-item\">Enzyme Mix 2 (blue cap): 1 x 340 \u00b5l. Enzyme mix for Index PCR.<\/span>\n                <span class=\"dlw-list-item\">Amp Mix V1-V3: 1 x 70 \u00b5l. Only for Microbiota Solution A; amplification of the V1-V2-V3 regions of the bacterial 16S rDNA gene.<\/span>\n                <span class=\"dlw-list-item\">Amp Mix V3-V6: 1 x 70 \u00b5l. Only for Microbiota Solution B; amplification of the V3-V4-V6 regions of the bacterial 16S rDNA gene.<\/span>\n                <span class=\"dlw-list-item\">Index Strip S1: 3 strips. Only for Microbiota Solution A; lyophilized oligonucleotide mix for indexing.<\/span>\n                <span class=\"dlw-list-item\">Index Strip S4: 3 strips. Only for Microbiota Solution B; lyophilized oligonucleotide mix for indexing.<\/span>\n                <span class=\"dlw-list-item\">Index Plate S12: 96-index plate, not supplied, but available upon request; lyophilized oligonucleotide mix for indexing amplified samples.<\/span>\n            <\/div>\n        <\/div>\n    <\/details>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Required materials not included<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <p>Bacterial genomic DNA extraction kit, AMPure Beads XP, MiSeq Reagent Nano Kit v2 (500-cycles), PhiX Control v3, molecular grade water, ethanol, and specific laboratory equipment, including thermal cycler and Illumina MiSeq sequencer.<\/p>\n        <\/div>\n    <\/details>\n\n    <!-- Estructura del Modal (Lightbox) -->\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n    <\/div>\n\n<\/div>\n\n<!-- Script del Modal (Vainilla JS, sin dependencias) -->\n<script>\n    function openDlwModal(src) {\n        var modal = document.getElementById('dlw-image-modal');\n        var modalImg = document.getElementById('dlw-modal-img-target');\n        modalImg.src = src;\n        modal.classList.add('dlw-active');\n        document.body.style.overflow = 'hidden'; \/\/ Bloquea el scroll del fondo\n    }\n\n    function closeDlwModal() {\n        var modal = document.getElementById('dlw-image-modal');\n        modal.classList.remove('dlw-active');\n        document.body.style.overflow = 'auto'; \/\/ Restaura el scroll\n    }\n\n    \/\/ Cierra el modal si haces clic fuera de la imagen\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n        if (e.target === this) {\n            closeDlwModal();\n        }\n    });\n\n    \/\/ Cierra el modal con la tecla ESC\n    document.addEventListener('keydown', function(e) {\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n            closeDlwModal();\n        }\n    });\n<\/script>\n\n","protected":false},"excerpt":{"rendered":"<p>In vitro diagnostic kit based on PCR and Next-Generation Sequencing (NGS) for the qualitative evaluation of the microbiome through the analysis of hypervariable regions of the bacterial 16S rDNA gene. It is designed for human gut microbiota and other biological samples, and can be used as a diagnostic aid in the clinical context of fecal [&hellip;]<\/p>\n","protected":false},"featured_media":27501,"template":"","tecnologias":[36],"marcas":[218],"familias":[328,329],"class_list":["post-27504","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-arrow-diagnostics-en","familias-microbiology","familias-bacteria"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27504\/revisions"}],"predecessor-version":[{"id":27505,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27504\/revisions\/27505"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27501"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27504"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27504"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27504"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}