{"id":27518,"date":"2026-05-21T08:31:54","date_gmt":"2026-05-21T08:31:54","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27518"},"modified":"2026-05-21T08:38:12","modified_gmt":"2026-05-21T08:38:12","slug":"mycobiota-solution","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/mycobiota-solution\/","title":{"rendered":"Mycobiota Solution"},"content":{"rendered":"\n<!-- Bloque de Estilos Scoped (Aislados, Blindados y Escalados a 14px) -->\n<style>\n    \/* Importaci\u00f3n de fuentes corporativas *\/\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n    \/* Contenedor Principal Aislado *\/\n    .dlw-prod-wrapper {\n        font-family: 'Raleway', sans-serif !important;\n        color: #000000 !important;\n        line-height: 1.6 !important;\n        background-color: transparent !important;\n       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========================================== -->\n<div class=\"dlw-prod-wrapper\">\n\n    <!-- Descripci\u00f3n Corta (Lead Box) -->\n    <div class=\"dlw-lead-box\">\n        <p>Kit for the qualitative evaluation of the human mycobiota by PCR amplification of the <strong>ITS1 region of fungal ribosomal DNA (rDNA)<\/strong> and <strong>NGS<\/strong> sequencing.<\/p>\n    <\/div>\n\n    <!-- Descripci\u00f3n Detallada -->\n    <h2>Detailed Description<\/h2>\n\n    <h3>Operating Principle<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Mycobiota Solution is based on the PCR amplification of the hypervariable <strong>ITS1<\/strong> region of the fungal rDNA, using primers that allow the identification of most fungal populations present in the sample. The generated amplicons are subsequently sequenced by <strong>Next Generation Sequencing (NGS)<\/strong>.<\/span>\n        <span class=\"dlw-list-item\">The protocol includes eight stages: DNA extraction and quantification, Target PCR, purification of Target PCR products, Index PCR, purification of Index PCR products, normalization and pool preparation, sequencing, and data analysis.<\/span>\n    <\/div>\n\n    <!-- Imagen 1 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen1-eng-8.webp\" alt=\"Operating Principle Mycobiota Solution\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n    <h3>Clinical Applications<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The kit is intended for the qualitative evaluation of the <strong>human mycobiota<\/strong> given its relevance in multiple physiological conditions and pathological states (for example, mechanisms of colorectal carcinogenesis and fungal infections in immunocompromised patients).<\/span>\n    <\/div>\n\n    <h3>Benefits<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Qualitative, rapid (~<strong>8 hours<\/strong>), and highly sensitive DNA-based evaluation of the mycobiota, without relying on morphological analysis by culture.<\/span>\n        <span class=\"dlw-list-item\">Automated post-sequencing analysis with dedicated software that generates reports on the fungal species identified in each sample and comparisons between samples selected by the user.<\/span>\n    <\/div>\n\n    <h3>Technology Used<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\"><strong>PCR<\/strong> amplification of the <strong>fungal rDNA ITS1<\/strong> region followed by <strong>NGS<\/strong>.<\/span>\n    <\/div>\n\n    <!-- Imagen 2 -->\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen2-eng-5.webp\" alt=\"Mycobiota Solution Workflow\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n    <h3>Target Audience\/User<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">This product is aimed at clinical microbiology or research laboratories that require a solution based on Next-Generation Sequencing (NGS) for the characterization of the human mycobiota. It is especially oriented towards users who need a protocol from library preparation to bioinformatic analysis for the study of fungal communities without relying on culture.<\/span>\n    <\/div>\n\n    <h3>Considerations or Limitations<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The kit is intended for <strong>manual use<\/strong>; any automation must be validated by the user.<\/span>\n        <span class=\"dlw-list-item\">Data analysis with MicrobAT provides taxonomic assignment down to the species level for most reads, along with descriptive statistics such as rarefaction curves, alpha-diversity, and dysbiosis index.<\/span>\n    <\/div>\n\n    <h3>Other Relevant Technical Information<\/h3>\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Recommended extraction methods: <strong>QIAamp PowerFecal Pro DNA Kit<\/strong> or <strong>QIAamp Fast DNA Stool Mini Kit<\/strong> for feces, and <strong>DNeasy PowerSoil Pro Kit<\/strong> for other samples; other methods may be used following user validation.<\/span>\n        <span class=\"dlw-list-item\">Requires a <strong>DNA input of 5\u201350 ng\/\u00b5l<\/strong>.<\/span>\n        <span class=\"dlw-list-item\">Compatibility with <strong>Illumina MiSeq&#x2122;, iSeq 100&#x2122; and MiniSeq&#x2122;<\/strong> platforms.<\/span>\n        <span class=\"dlw-list-item\">For optimal coverage of the amplified region, it is recommended to obtain <strong>at least 50,000 reads per sample<\/strong>.<\/span>\n    <\/div>\n\n    <!-- Aspectos Clave -->\n    <h2>Key Aspects<\/h2>\n\n    <div class=\"dlw-check-list\">\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Qualitative evaluation of the human mycobiota via <strong>ITS1 PCR + NGS<\/strong>.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Library preparation ~8 hours.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Applicable to samples such as feces, rectal, vaginal, skin and oral swabs, saliva, sputum, and urine.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Avoids the culture phase and reduces the bias associated with laboratory culture conditions.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Dedicated software for per-sample and comparative reports, with descriptive diversity metrics.<\/span>\n        <\/div>\n        <div class=\"dlw-check-item\">\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n            <span>Compatible with <strong>Illumina MiSeq&#x2122;, Illumina iSeq 100&#x2122; and Illumina MiniSeq&#x2122;<\/strong>.<\/span>\n        <\/div>\n    <\/div>\n\n    <!-- Detalles de Presentaci\u00f3n (Acordeones Desplegables) -->\n    <h2>Presentation Details<\/h2>\n\n    <details class=\"dlw-accordion\">\n        <summary class=\"dlw-accordion-header\">Format and References<\/summary>\n        <div class=\"dlw-accordion-content\">\n            <table class=\"dlw-table\">\n                <tbody>\n                    <tr>\n                        <td width=\"30%\" class=\"dlw-ref-code\">AD-005.048<\/td>\n                        <td width=\"70%\"><strong>48 reactions<\/strong><\/td>\n                    <\/tr>\n                <\/tbody>\n            <\/table>\n        <\/div>\n    <\/details>\n\n    <!-- Estructura del Modal (Lightbox) -->\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n    <\/div>\n\n<\/div>\n\n<!-- Script del Modal (Vainilla JS, sin dependencias) -->\n<script>\n    function openDlwModal(src) {\n        var modal = document.getElementById('dlw-image-modal');\n        var modalImg = document.getElementById('dlw-modal-img-target');\n        modalImg.src = src;\n        modal.classList.add('dlw-active');\n        document.body.style.overflow = 'hidden'; 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Detailed Description Operating Principle Mycobiota Solution is based on the PCR amplification of the hypervariable ITS1 region of the fungal rDNA, using primers that allow the identification of most fungal populations [&hellip;]<\/p>\n","protected":false},"featured_media":27514,"template":"","tecnologias":[36],"marcas":[218],"familias":[328,332],"class_list":["post-27518","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-arrow-diagnostics-en","familias-microbiology","familias-fungus"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27518","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27518\/revisions"}],"predecessor-version":[{"id":27519,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27518\/revisions\/27519"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27514"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27518"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27518"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27518"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27518"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}