{"id":27541,"date":"2026-05-22T09:55:46","date_gmt":"2026-05-22T09:55:46","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27541"},"modified":"2026-05-22T09:59:35","modified_gmt":"2026-05-22T09:59:35","slug":"illumina-covidseq","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/illumina-covidseq\/","title":{"rendered":"Illumina COVIDSeq"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded, and Scaled to 14px) -->\n\n<style>\n\n    \/* Corporate font import *\/\n\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n\n\n    \/* Main Isolated Container *\/\n\n    .dlw-prod-wrapper {\n\n        font-family: 'Raleway', sans-serif !important;\n\n        color: #000000 !important;\n\n        line-height: 1.6 !important;\n\n        background-color: transparent !important;\n\n        padding: 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thead tr { position: absolute !important; top: -9999px !important; left: -9999px !important; }\n\n        .dlw-table tr { border-bottom: 2px solid var(--dlw-blue-cyan) !important; margin-bottom: 15px !important; }\n\n        .dlw-table td { border: none !important; position: relative !important; padding-left: 45% !important; }\n\n        .dlw-table td::before { position: absolute !important; top: 15px !important; left: 15px !important; width: 40% !important; white-space: nowrap !important; font-weight: bold !important; color: var(--dlw-blue-dark) !important; font-size: 0.9em !important; text-transform: uppercase !important; }\n\n        .dlw-table td:nth-of-type(1)::before { content: \"Reference\" !important; }\n\n        .dlw-table td:nth-of-type(2)::before { content: \"Description\" !important; }\n\n    }\n\n<\/style>\n\n\n\n<!-- ==========================================\n\n     UNIFIED AND SHIELDED CONTENT\n\n     ========================================== -->\n\n<div class=\"dlw-prod-wrapper\">\n\n\n\n    <!-- Short Description (Lead Box) -->\n\n    <div class=\"dlw-lead-box\">\n\n        <p>Next-generation sequencing (NGS) amplicon-based assay for the detection and characterization of SARS-CoV-2. It integrates library preparation, sequencing, and data analysis into a workflow aimed at genomic surveillance and is for research use only.<\/p>\n\n    <\/div>\n\n\n\n    <!-- Detailed Description -->\n\n    <h2>3.- Detailed Description<\/h2>\n\n\n\n    <h3>Principle of operation<\/h3>\n\n    <p>Illumina COVIDSeq is integrated into a complete workflow for the detection and characterization of SARS-CoV-2, from RNA isolation to library preparation, sequencing, and bioinformatic analysis. The process comprises annealing of extracted RNA using random hexamers, first-strand cDNA synthesis, amplification of the material in two PCR reactions, amplicon tagmentation, subsequent clean\u2011up, incorporation of i7 and i5 indexes, pool formation, and final library clean\u2011up, with the aim of generating sequencing\u2011ready libraries from SARS\u2011CoV\u20112 samples.<\/p>\n\n\n\n    <h3>Clinical applications<\/h3>\n\n    <p>The documentation places this assay in the field of SARS\u2011CoV\u20112 RNA sequencing and monitoring of viral genome characteristics, allowing the identification, tracking, and monitoring of the emergence and prevalence of new variants, strains, and lineages from samples originating from nasopharyngeal, oropharyngeal, and nasal swabs. Its application is oriented to research and clinical research environments linked to genomic surveillance.<\/p>\n\n\n\n    <h3>Benefits<\/h3>\n\n    <p>Among its main advantages is its integrated and optimized workflow, designed to adapt flexibly to different laboratory settings, especially in research and clinical research, by combining library preparation, sequencing, and data analysis into a single process. Added to this is its suitability for decentralized SARS\u2011CoV\u20112 surveillance strategies and its ability to offer uniform coverage of the viral genome, with special relevance in the spike protein region, key for variant characterization. As a result, the assay allows SARS\u2011CoV\u20112 detection, alignment against the reference genome, variant calling, generation of consensus sequences, and assignment of viral lineages and subtypes.<\/p>\n\n\n\n    <h3>Key results or indicators<\/h3>\n\n    <p>Illumina COVIDSeq provides uniform coverage of the SARS\u2011CoV\u20112 genome, with particular robustness in the S protein region, key for variant identification and characterization. Illumina&#8217;s documentation shows that ARTIC v5.4.2 primers improve coverage of new Omicron variants, especially in the spike region, favoring a more complete and accurate characterization of emerging variants. Illumina indicates that paired\u2011end sequencing strategies improve performance over single\u2011read and that, in the samples evaluated, genomic coverages \u2265 99% can be achieved with 2 \u00d7 76 bp configurations or higher and depths of at least 1 million reads per sample, reinforcing the reliability of the assay for genomic surveillance programs and monitoring of viral lineages.<\/p>\n\n\n\n    <h3>Technology used<\/h3>\n\n    <p>This is a next\u2011generation sequencing (NGS) amplicon\u2011based assay that includes the reagents necessary for RNA\u2011to\u2011cDNA conversion, amplification, and library preparation, and incorporates ARTIC v5.4.2 primers for the detection and characterization of SARS\u2011CoV\u20112. Regarding bioinformatic analysis, the COVIDSeq workflow relies on Illumina&#8217;s DRAGEN tools in BaseSpace Sequence Hub, which allow aligning reads against a reference genome, performing variant calling, generating a consensus sequence, and obtaining lineage and subtype information for genomic surveillance applications.<\/p>\n\n\n\n    <h3>Intended user \/ audience<\/h3>\n\n    <p>This assay is aimed at laboratories that need a SARS\u2011CoV\u20112 sequencing solution working in clinical research, genomic characterization, and variant surveillance, especially in environments that use Illumina sequencing platforms and require a structured workflow from library preparation to bioinformatic analysis.<\/p>\n\n\n\n    <h3>Considerations or limitations<\/h3>\n\n    <p>The product is for research use only and must not be used in diagnostic procedures. Pre\u2011analytical sample quality, including collection, transport, and storage conditions, as well as the stage of infection and possible RNA degradation, can compromise SARS\u2011CoV\u20112 detection, so the protocol requires rigorous contamination control and proper handling of material to preserve its integrity.<\/p>\n\n\n\n    <!-- Key Features -->\n\n    <h2>4.- Key Features<\/h2>\n\n    \n\n    <div class=\"dlw-check-list\">\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>NGS amplicon\u2011based assay for SARS\u2011CoV\u20112 detection and characterization.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Integrated workflow from RNA extraction to data analysis.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Up to 96 samples per kit preparation capacity.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Uniform viral genome coverage, with special relevance in the spike protein region.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>ARTIC v5.4.2 primers that improve coverage of new variants.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Suitable for decentralized genomic surveillance on Illumina sequencing platforms.<\/span>\n\n        <\/div>\n\n    <\/div>\n\n\n\n    <!-- Presentation Details (Collapsible Accordions) -->\n\n    <h2>5.- Presentation Details<\/h2>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Format<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <p>Sequencing assay with capacity for 96 and 3072 samples.<\/p>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Kit Contents<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">The content is distributed across several boxes including reagents such as Illumina Tune Beads (ITB), Stop Tagment Buffer 2 (ST2), Enrichment BLT (EBLTS), Elution Buffer (ELB), Resuspension Buffer (RSB), Tagmentation Wash Buffer (TWB), COVIDSeq Primer Pool 1 (CPP1), COVIDSeq Primer Pool 2 (CPP2), Elution Prime Fragment 3HC Mix (EPH3), Enhanced PCR Mix (EPM), First Strand Mix (FSM), Illumina PCR Mix (IPM), Reverse Transcriptase (RVT) and Tagmentation Buffer 1 (TB1), as well as IDT for Illumina-PCR Indexes.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">References<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <table class=\"dlw-table\">\n\n                <tbody>\n\n                    <tr>\n\n                        <td width=\"30%\" class=\"dlw-ref-code\">Illumina COVIDSeq Assay (96 Samples)<\/td>\n\n                        <td width=\"70%\">\u2014<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Assay Box 1 \u2013 96 Samples<\/td>\n\n                        <td>Part # 20051272<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Assay Box 2 \u2013 96 Samples<\/td>\n\n                        <td>Part # 20051273<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Assay Box 3 \u2013 96 Samples<\/td>\n\n                        <td>Part # 20051274<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Assay Box 4 \u2013 96 Samples, Indexes<\/td>\n\n                        <td>\u2014<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Positive Control HT<\/td>\n\n                        <td>Part # 20051775<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Test (RUO) Kit Contents (3072 Samples)<\/td>\n\n                        <td>\u2014<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Test Box 1 \u2013 3072 Samples<\/td>\n\n                        <td>Part # 20044405<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Test Box 2 \u2013 3072 Samples<\/td>\n\n                        <td>Part # 20044406<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Test Box 3 \u2013 3072 Samples<\/td>\n\n                        <td>Part # 20044407<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina COVIDSeq Positive Control HT<\/td>\n\n                        <td>Part # 20043401<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">IDT for Illumina-PCR Indexes<\/td>\n\n                        <td>\u2014<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">IDT for Illumina-PCR Indexes Set 1 (96 Indexes)<\/td>\n\n                        <td>Part # 20043132<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">IDT for Illumina-PCR Indexes Set 2 (96 Indexes)<\/td>\n\n                        <td>Part # 20043132<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">IDT for Illumina-PCR Indexes Set 3 (96 Indexes)<\/td>\n\n                        <td>Part # 20043132<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">IDT for Illumina-PCR Indexes Set 4 (96 Indexes)<\/td>\n\n                        <td>Part # 20043132<\/td>\n\n                    <\/tr>\n\n                <\/tbody>\n\n            <\/table>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <!-- Modal Structure (Lightbox) -->\n\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n\n    <\/div>\n\n\n\n<\/div>\n\n\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n\n<script>\n\n    function openDlwModal(src) {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        var modalImg = document.getElementById('dlw-modal-img-target');\n\n        modalImg.src = src;\n\n        modal.classList.add('dlw-active');\n\n        document.body.style.overflow = 'hidden'; \/\/ Locks background scroll\n\n    }\n\n\n\n    function closeDlwModal() {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        modal.classList.remove('dlw-active');\n\n        document.body.style.overflow = 'auto'; \/\/ Restores scroll\n\n    }\n\n\n\n    \/\/ Closes modal if clicking outside the image\n\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n\n        if (e.target === this) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n\n\n    \/\/ Closes modal with ESC key\n\n    document.addEventListener('keydown', function(e) {\n\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n<\/script>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Next-generation sequencing (NGS) amplicon-based assay for the detection and characterization of SARS-CoV-2. It integrates library preparation, sequencing, and data analysis into a workflow aimed at genomic surveillance and is for research use only. 3.- Detailed Description Principle of operation Illumina COVIDSeq is integrated into a complete workflow for the detection and characterization of SARS-CoV-2, from [&hellip;]<\/p>\n","protected":false},"featured_media":27539,"template":"","tecnologias":[36],"marcas":[232],"familias":[328,335],"class_list":["post-27541","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-illumina-en","familias-microbiology","familias-virus-en"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27541","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27541\/revisions"}],"predecessor-version":[{"id":27542,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27541\/revisions\/27542"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27539"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27541"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27541"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27541"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27541"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}