{"id":27554,"date":"2026-05-22T10:13:13","date_gmt":"2026-05-22T10:13:13","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27554"},"modified":"2026-05-22T10:16:22","modified_gmt":"2026-05-22T10:16:22","slug":"illumina-microbial-amplicon-prep-imap","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/illumina-microbial-amplicon-prep-imap\/","title":{"rendered":"Illumina Microbial Amplicon Prep (IMAP)"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded, and Scaled to 14px) -->\n\n<style>\n\n    \/* Corporate font import *\/\n\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n\n\n    \/* Main Isolated Container *\/\n\n    .dlw-prod-wrapper {\n\n        font-family: 'Raleway', sans-serif !important;\n\n        color: #000000 !important;\n\n        line-height: 1.6 !important;\n\n        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========================================== -->\n\n<div class=\"dlw-prod-wrapper\">\n\n\n\n    <!-- Short Description (Lead Box) -->\n\n    <div class=\"dlw-lead-box\">\n\n        <p>Amplicon-based NGS library preparation solution aimed at public health surveillance and microbiological research. Accepts DNA or RNA, as well as user-designed or commercial primers, with compatibility with multiple sample types and with almost all Illumina sequencing systems.<\/p>\n\n    <\/div>\n\n\n\n    <!-- Detailed Description -->\n\n    <h2>Detailed Description<\/h2>\n\n\n\n    <h3>Principle of operation<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Amplicon-based library preparation kit built on the same chemistry as COVIDSeq. The workflow integrates, depending on the sample type, cDNA conversion for RNA targets, multiplex PCR amplification, tagmentation, library amplification, bead clean\u2011up, and indexing. After sequencing, data can be analyzed with DRAGEN Targeted Microbial on BaseSpace Sequence Hub for alignment, variant calling, and consensus sequence generation.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Applications<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The documentation places it in genomic surveillance of infectious diseases, public health, and microbiological research. Its use is mentioned for viral whole\u2011genome sequencing, antimicrobial resistance marker analysis, bacterial and fungal identification, variant detection, lineage\/strain classification, and pathogen tracking over time and across different geographic areas.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Benefits<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">It offers a fast and optimized workflow, with a total assay time < 9 hours and a hands\u2011on time of approximately 3 hours for 48 samples. It supports scalable preparation from 1 to 48 samples, RNA or DNA inputs, and compatibility with automation using liquid handling robots.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Key results or indicators<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">In the application note for viral surveillance, Illumina summarizes comprehensive genomic coverage in the evaluated viruses, defined as > 90% of the viral genome covered at \u2265 10\u00d7. For arboviruses, median coverage at \u2265 10\u00d7 of 80% and 96% are reported for chikungunya, 94% and 98.5% for dengue 1, and 97.2% and 98.5% for zika, with 500 and 5000 input copies, respectively. In mpox, robust coverage was observed with 1 million paired\u2011end reads, although low\u2011titer samples showed lower coverage; in hRSV A\/B, both primer designs amplified the genomes, but the longer amplicon (~4300 bp) exhibited lower coverage depth.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Technology used<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">It employs multiplex PCR\u2011based amplicon sequencing and NGS, with secondary analysis using DRAGEN Targeted Microbial. It is compatible with published, commercial, or laboratory\u2011designed primers; the documentation recommends 400 bp amplicons as a starting point, although longer amplicons may be used depending on the target. Libraries can be sequenced on multiple Illumina platforms; the data sheet recommends read lengths of 2 \u00d7 101 bp and 2 \u00d7 151 bp.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Input and sample requirements<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Compatible with DNA or RNA extracted from microbial cultures, nasopharyngeal, skin or nasal swabs, wastewater, and other sources. Input amount varies depending on sample origin. The protocol provides purity guidelines: A260\/280 of 2.0\u20132.2 for RNA and 1.8\u20132.0 for DNA, and A260\/230 of 2.0\u20132.2 for both. For inputs consisting solely of purified RNA, Illumina recommends DNase treatment during purification.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Intended user \/ audience<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Aimed at public health, microbiology, and infectious disease laboratories and researchers requiring targeted sequencing of microorganisms for surveillance and genomic characterization. The documentation classifies it as for research use only and not suitable for diagnostic procedures.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Considerations or limitations<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Target\u2011specific primers are not included and must be purchased separately; their design directly influences assay performance. Illumina recommends working with high\u2011quality nucleic acids and notes that substitutions at primer binding sites may reduce coverage or cause amplicon dropout. It also indicates that low\u2011titer samples may not achieve complete coverage and that some targets may require adjustments to the protocol, primer design, or PCR conditions. Customer\u2011provided protocols on the website are offered for informational purposes only and do not imply validation or support by Illumina.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Key Features -->\n\n    <h2>Key Features<\/h2>\n\n    \n\n    <div class=\"dlw-check-list\">\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Amplicon\u2011based library preparation for microbiological surveillance and public health.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Compatible with DNA and RNA and with a wide variety of sample types.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Accepts user\u2011designed, published, or commercial primers.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Fast workflow: < 9 h assay and ~3 h hands\u2011on for 48 samples.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Scalable preparation from 1 to 48 indexed libraries.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Integration with DRAGEN Targeted Microbial for alignment, variants, and consensus genome.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Proven performance on RNA and DNA viruses, with broad coverage in the evaluated examples.<\/span>\n\n        <\/div>\n\n    <\/div>\n\n\n\n    <!-- Presentation Details (Collapsible Accordions) -->\n\n    <h2>Presentation Details<\/h2>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Format<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <p>Kit for 48 samples. The documentation describes up to 48 uniquely indexed targeted libraries.<\/p>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Functional kit contents<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <p>Reagents for cDNA conversion, amplification, tagmentation, library clean\u2011up\/purification, and indexing. Primer oligonucleotides are purchased separately.<\/p>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Content breakdown according to the protocol<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">Box 1: Illumina Purification Beads (IPB) and Stop Tagment Buffer 2 (ST2).<\/span>\n\n                <span class=\"dlw-list-item\">Box 2: Enrichment BLT (EBLTS) and Tagmentation Wash Buffer (TWB).<\/span>\n\n                <span class=\"dlw-list-item\">Box 3: Elution Prime Fragment 3HC Mix (EPH3), Enhanced PCR Mix (EPM), First Strand Mix (FSM), Illumina PCR Mix (PM), Resuspension Buffer (RSB), Reverse Transcriptase (RVT) and Tagmentation Buffer 1 (TB1).<\/span>\n\n                <span class=\"dlw-list-item\">Box 4: Illumina Unique Dual Indexes, LT.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Commercial reference<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <table class=\"dlw-table\">\n\n                <tbody>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina Microbial Amplicon Prep (48 samples)<\/td>\n\n                        <td>Cat. 20097857<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina Unique Dual Indexes, LT<\/td>\n\n                        <td>Cat. 20098166<\/td>\n\n                    <\/tr>\n\n                <\/tbody>\n\n             <\/table>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <!-- Modal Structure (Lightbox) -->\n\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n\n    <\/div>\n\n\n\n<\/div>\n\n\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n\n<script>\n\n    function openDlwModal(src) {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        var modalImg = document.getElementById('dlw-modal-img-target');\n\n        modalImg.src = src;\n\n        modal.classList.add('dlw-active');\n\n        document.body.style.overflow = 'hidden'; \/\/ Locks background scroll\n\n    }\n\n\n\n    function closeDlwModal() {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        modal.classList.remove('dlw-active');\n\n        document.body.style.overflow = 'auto'; \/\/ Restores scroll\n\n    }\n\n\n\n    \/\/ Closes modal if clicking outside the image\n\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n\n        if (e.target === this) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n\n\n    \/\/ Closes modal with ESC key\n\n    document.addEventListener('keydown', function(e) {\n\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n<\/script>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Amplicon-based NGS library preparation solution aimed at public health surveillance and microbiological research. Accepts DNA or RNA, as well as user-designed or commercial primers, with compatibility with multiple sample types and with almost all Illumina sequencing systems. Detailed Description Principle of operation Amplicon-based library preparation kit built on the same chemistry as COVIDSeq. The workflow [&hellip;]<\/p>\n","protected":false},"featured_media":27550,"template":"","tecnologias":[36],"marcas":[232],"familias":[328,329,332,335],"class_list":["post-27554","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-illumina-en","familias-microbiology","familias-bacteria","familias-fungus","familias-virus-en"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27554","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27554\/revisions"}],"predecessor-version":[{"id":27556,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27554\/revisions\/27556"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27550"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27554"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27554"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27554"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27554"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}