{"id":27559,"date":"2026-05-22T10:22:25","date_gmt":"2026-05-22T10:22:25","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27559"},"modified":"2026-05-22T10:24:57","modified_gmt":"2026-05-22T10:24:57","slug":"illumina-microbial-amplicon-prep-influenza-a-b-imap-flu","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/illumina-microbial-amplicon-prep-influenza-a-b-imap-flu\/","title":{"rendered":"Illumina Microbial Amplicon Prep\u2013Influenza A\/B (IMAP-Flu)"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded, and Scaled to 14px) -->\n\n<style>\n\n    \/* Corporate font import *\/\n\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n\n\n    \/* Main Isolated Container *\/\n\n    .dlw-prod-wrapper {\n\n        font-family: 'Raleway', sans-serif !important;\n\n        color: #000000 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tbody, .dlw-table th, .dlw-table td, .dlw-table tr { display: block !important; }\n\n        .dlw-table thead tr { position: absolute !important; top: -9999px !important; left: -9999px !important; }\n\n        .dlw-table tr { border-bottom: 2px solid var(--dlw-blue-cyan) !important; margin-bottom: 15px !important; }\n\n        .dlw-table td { border: none !important; position: relative !important; padding-left: 45% !important; }\n\n        .dlw-table td::before { position: absolute !important; top: 15px !important; left: 15px !important; width: 40% !important; white-space: nowrap !important; font-weight: bold !important; color: var(--dlw-blue-dark) !important; font-size: 0.9em !important; text-transform: uppercase !important; }\n\n        .dlw-table td:nth-of-type(1)::before { content: \"Reference\" !important; }\n\n        .dlw-table td:nth-of-type(2)::before { content: \"Description\" !important; }\n\n    }\n\n<\/style>\n\n\n\n<!-- ==========================================\n\n     UNIFIED AND SHIELDED CONTENT\n\n     ========================================== -->\n\n<div class=\"dlw-prod-wrapper\">\n\n\n\n    <!-- Short Description (Lead Box) -->\n\n    <div class=\"dlw-lead-box\">\n\n        <p>Targeted library preparation workflow for whole\u2011genome sequencing of influenza A and influenza B from total RNA on Illumina systems. Enables preparation of up to 48 libraries with unique dual indexes and generates sequencing\u2011ready libraries in less than 9 hours.<\/p>\n\n    <\/div>\n\n\n\n    <!-- Detailed Description -->\n\n    <h2>Detailed Description<\/h2>\n\n\n\n    <h3>Principle of operation<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The IMAP-Flu workflow uses a pool of 16 primers (Influenza A\/B Primer Pool) to hybridize to the 3&#8242; ends of genomic RNA segments and cDNA, allowing both cDNA synthesis and subsequent PCR amplification to generate whole\u2011genome amplicons ready for tagmentation. It is indicated that it amplifies all 8 genomic RNA segments from each sample.<\/span>\n\n        <span class=\"dlw-list-item\">After amplification, amplicons are fragmented and tagged by tagmentation with EBLTS. Then, an indexing PCR adds unique 10 bp dual indexes for i7 and i5, together with the sequences necessary for sequencing cluster generation.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Benefits<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Preparation of up to 48 uniquely dual\u2011indexed libraries.<\/span>\n\n        <span class=\"dlw-list-item\">Scalable workflow from 1 to 48 samples.<\/span>\n\n        <span class=\"dlw-list-item\">Generation of sequencing\u2011ready libraries in less than 9 hours.<\/span>\n\n        <span class=\"dlw-list-item\">Assay for influenza A and influenza B, with the ability to analyze subtypes of both viruses.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Key results or indicators<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The workflow output is a targeted whole\u2011genome library of influenza A\/B from RNA, prepared for sequencing on Illumina systems.<\/span>\n\n        <span class=\"dlw-list-item\">Illumina recommends samples with Ct < 30 to achieve high sequencing depth and complete genome coverage.<\/span>\n\n        <span class=\"dlw-list-item\">For quantification and normalization, the documentation indicates analyzing 2 \u00b5l of the pool with the Qubit dsDNA HS Assay Kit and normalizing the library to 4 nM, with a minimum volume of 30 \u00b5l. The average library size used for molarity calculation is around 400 bp.<\/span>\n\n        <span class=\"dlw-list-item\">Maximum sequencing capacities with 300\u2011cycle cartridges for iSeq and MiSeq: iSeq up to 20 samples; MiSeq Nano v2 up to 5, Micro v2 up to 20, and Standard v2 up to 72 samples. Use with MiniSeq and NextSeq is also indicated.<\/span>\n\n        <span class=\"dlw-list-item\">In Illumina&#8217;s documentation, as an example of normalized pooling with 1 million paired\u2011end reads per sample, capacities of 8 samples on iSeq 100 v1\/v2, 30 on MiSeq v2, 48 on MiniSeq HO, and 384 on NextSeq 500\/550 HO are provided, using multiple kits and additional indexes.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Technology used<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Universal whole\u2011genome RT\u2011PCR for influenza A\/B.<\/span>\n\n        <span class=\"dlw-list-item\">Amplicon tagmentation with adapter addition.<\/span>\n\n        <span class=\"dlw-list-item\">Indexing PCR with unique dual indexes.<\/span>\n\n        <span class=\"dlw-list-item\">Qubit quantification and normalization prior to sequencing.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Considerations or limitations<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Results may vary depending on viral load and are not guaranteed.<\/span>\n\n        <span class=\"dlw-list-item\">To maximize library preparation success, RNA samples with A260\/280 and A260\/230 ratios of 2.0\u20132.2 are recommended. Values outside this range may indicate contaminants and cause assay failure.<\/span>\n\n        <span class=\"dlw-list-item\">The documentation indicates that the protocol has been optimized and validated with specific consumables and equipment, and comparable performance with alternatives is not guaranteed.<\/span>\n\n        <span class=\"dlw-list-item\">The kit should not be subjected to more than 8 freeze\u2011thaw cycles.<\/span>\n\n        <span class=\"dlw-list-item\">It is recommended to work in an RNase\u2011\/DNase\u2011free environment, use negative controls, and avoid amplicon carryover contamination. Pooled libraries are stable for up to 30 days at -25 \u00b0C to -15 \u00b0C.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Key Features -->\n\n    <h2>Key Features<\/h2>\n\n    \n\n    <div class=\"dlw-check-list\">\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Targeted whole\u2011genome sequencing of influenza A\/B from total RNA.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Up to 48 uniquely dual\u2011indexed libraries per kit.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Scalable preparation workflow from 1 to 48 samples.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Sequencing\u2011ready libraries in less than 9 hours.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Pool of 16 primers for universal whole\u2011genome amplification.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Recommendation of Ct < 30 for greater depth and complete coverage.<\/span>\n\n        <\/div>\n\n    <\/div>\n\n\n\n    <!-- Presentation Details (Collapsible Accordions) -->\n\n    <h2>Presentation Details<\/h2>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Format<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <p>Library preparation kit for 48 samples, distributed in five boxes.<\/p>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Kit Contents<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">Box 1 (room temperature), component #20093449: IPB (Illumina Purification Beads) and ST2 (Stop Tagment Buffer 2).<\/span>\n\n                <span class=\"dlw-list-item\">Box 2 (2 \u00b0C to 8 \u00b0C), component #20093450: EBLTS (Enrichment BLT), TWB (Tagmentation Wash Buffer) and ELB (Elution Buffer).<\/span>\n\n                <span class=\"dlw-list-item\">Box 3 (-25 \u00b0C to -15 \u00b0C), component #20093451: EPH3, EPM, FSM, IPM, RSB, RVT and TB1. The documentation indicates that EPH3 is not used in the IMAP-Flu protocol.<\/span>\n\n                <span class=\"dlw-list-item\">Illumina Unique Dual Indexes, LT, box #20083060: 1 set of unique dual indexes.<\/span>\n\n                <span class=\"dlw-list-item\">Influenza A\/B Primer Pool, box #20108095: 1 IPP (Influenza A\/B Primer Pool).<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Storage conditions<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <p>Reagents distributed among room temperature, refrigerated, and frozen storage depending on the component.<\/p>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <!-- Modal Structure (Lightbox) -->\n\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n\n    <\/div>\n\n\n\n<\/div>\n\n\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n\n<script>\n\n    function openDlwModal(src) {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        var modalImg = document.getElementById('dlw-modal-img-target');\n\n        modalImg.src = src;\n\n        modal.classList.add('dlw-active');\n\n        document.body.style.overflow = 'hidden'; \/\/ Locks background scroll\n\n    }\n\n\n\n    function closeDlwModal() {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        modal.classList.remove('dlw-active');\n\n        document.body.style.overflow = 'auto'; \/\/ Restores scroll\n\n    }\n\n\n\n    \/\/ Closes modal if clicking outside the image\n\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n\n        if (e.target === this) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n\n\n    \/\/ Closes modal with ESC key\n\n    document.addEventListener('keydown', function(e) {\n\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n<\/script>\n","protected":false},"excerpt":{"rendered":"<p>Targeted library preparation workflow for whole\u2011genome sequencing of influenza A and influenza B from total RNA on Illumina systems. Enables preparation of up to 48 libraries with unique dual indexes and generates sequencing\u2011ready libraries in less than 9 hours. Detailed Description Principle of operation The IMAP-Flu workflow uses a pool of 16 primers (Influenza A\/B [&hellip;]<\/p>\n","protected":false},"featured_media":27550,"template":"","tecnologias":[36],"marcas":[232],"familias":[328,335],"class_list":["post-27559","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-illumina-en","familias-microbiology","familias-virus-en"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27559","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27559\/revisions"}],"predecessor-version":[{"id":27561,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27559\/revisions\/27561"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27550"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27559"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27559"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27559"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27559"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}