{"id":27566,"date":"2026-05-22T10:29:44","date_gmt":"2026-05-22T10:29:44","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27566"},"modified":"2026-05-22T10:32:01","modified_gmt":"2026-05-22T10:32:01","slug":"illumina-viral-surveillance-panel-v2","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/illumina-viral-surveillance-panel-v2\/","title":{"rendered":"Illumina Viral Surveillance Panel v2"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded, and Scaled to 14px) -->\n\n<style>\n\n    \/* Corporate font import *\/\n\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n\n\n    \/* Main Isolated Container *\/\n\n    .dlw-prod-wrapper {\n\n        font-family: 'Raleway', sans-serif !important;\n\n        color: #000000 !important;\n\n        line-height: 1.6 !important;\n\n        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========================================== -->\n\n<div class=\"dlw-prod-wrapper\">\n\n\n\n    <!-- Short Description (Lead Box) -->\n\n    <div class=\"dlw-lead-box\">\n\n        <p>Next\u2011generation sequencing (NGS) assay with hybrid capture enrichment for the detection and whole\u2011genome sequencing of approximately 200 RNA and DNA viruses relevant to public health. It integrates library preparation, enrichment, sequencing, and data analysis into an optimized workflow of about two days.<\/p>\n\n    <\/div>\n\n\n\n    <!-- Detailed Description -->\n\n    <h2>Detailed Description<\/h2>\n\n\n\n    <h3>Principle of operation<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The panel can start from RNA, DNA, or total nucleic acid extracted from host or environmental samples. When the sample contains RNA, it is denatured and converted into double\u2011stranded cDNA; subsequently, the material is fragmented by tagmentation with Bead\u2011Linked Transposomes for Enrichment (EBLTL). Libraries are indexed with unique dual indexes, pooled, and subjected to hybrid\u2011capture enrichment with probes that cover the target viral genomes to generate whole\u2011genome sequencing data.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Clinical applications<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">It is aimed at outbreak genomic surveillance, zoonotic surveillance, mutation tracking, and variant monitoring. The workflow supports clinical and remnant clinical samples such as plasma, serum, skin lesions, and nasopharyngeal swabs, as well as environmental samples, including wastewater, for regional surveillance and early detection of viral sequences.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Benefits<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Compared to metagenomic shotgun sequencing, hybrid capture enrichment reduces unnecessary sequencing of host material and non\u2011target microorganisms, decreases the need for high read depths, reduces costs, and favors higher throughput. Additionally, the document indicates that, compared to targeted methods such as amplicon sequencing, hybrid capture offers more uniform coverage of the viral genome and greater capacity to identify mutations and divergent sequences. The workflow is compatible with automation, requires little hands\u2011on time, and can be completed in approximately two days.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Key results or indicators<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">In multiviric samples prepared with 1000 genomic copies per reaction, the panel recovered on average 99.1% of the Human adenovirus E genome and 99.4% of the Influenza A virus (H3N2) genome, while shotgun sequencing recovered on average 1.9% and 0%, respectively. In remnant clinical samples, all samples enriched with Viral Surveillance Panel v2 showed higher viral detection sensitivity than shotgun metagenomics. In wastewater, it also demonstrated higher detection sensitivity in complex samples with low viral load, even when shotgun sequencing increased the total number of reads approximately six\u2011fold.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Technology used<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">It uses NGS with hybrid capture target enrichment, tagmentation with EBLTL, unique dual indexing of up to 384 libraries, and analysis with DRAGEN Microbial Enrichment Plus App on BaseSpace Sequence Hub. Bioinformatic processing includes sample quality control, reference\u2011guided alignment against a curated viral database, variant calling, consensus sequence generation, antiviral resistance prediction for influenza A\/B, and integration with Pangolin and Nextclade for phylogenetic assignment in compatible viruses.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Intended user \/ audience<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">This assay is intended for public health organizations and researchers who need viral surveillance, viral genome characterization, and analysis of complex samples in research contexts.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Considerations or limitations<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">It is a product for research use only and not for diagnostic procedures. The protocol is optimized for 10\u2013100 ng of purified total RNA, DNA, or total nucleic acid; smaller amounts or lower quality material may reduce library yield. It is not recommended to normalize samples by equal mass; it is recommended to use the same volume of extracted nucleic acid per sample. It is recommended to pool samples with similar viral titers; mixing very different titers may bias results. In complex samples, such as wastewater, off\u2011target reads may be expected if other microbial nucleic acids are present.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Other relevant technical information<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The workflow supports sequencing on Illumina MiniSeq, MiSeq, NextSeq 550, NextSeq 1000, and NextSeq 2000 systems. For good quality samples, the general recommendation is a minimum of 2 million reads per sample with a read length of 2 \u00d7 150 bp; for wastewater, the document recommends a minimum of 8 million reads per sample.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Key Features -->\n\n    <h2>Key Features<\/h2>\n\n    \n\n    <div class=\"dlw-check-list\">\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Covers approximately 200 RNA and DNA viruses, including viruses of high public health relevance.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Hybrid capture enrichment for viral detection and whole\u2011genome sequencing with lower depth requirement than shotgun metagenomics.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Compatible with host and environmental samples, including plasma, serum, skin lesions, nasopharyngeal swabs, and wastewater.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Integrated workflow from library preparation to bioinformatic analysis in approximately 2 days with little hands\u2011on time.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Unique dual indexing and multiplexing capacity of up to 384 libraries.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Analysis with DRAGEN Microbial Enrichment Plus App and support for quality control, variants, consensi, and phylogenetic assignment.<\/span>\n\n        <\/div>\n\n    <\/div>\n\n\n\n    <!-- Presentation Details (Collapsible Accordions) -->\n\n    <h2>Presentation Details<\/h2>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Format<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <p>Targeted sequencing protocol for VSPv2 available as a kit in four 96\u2011sample sets. Each kit includes reagents for 32 reactions and, using the recommended 3\u2011plex format, can process 96 samples. Additionally, the panel can be purchased alone.<\/p>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Kit Contents<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">The content is distributed across several boxes including reagents such as Illumina Viral Surveillance Panel v2, Illumina RNA Prep with Enrichment (L) Tagmentation library preparation reagents, Illumina Purification Beads, and an Illumina DNA\/RNA UD Indexes adapter plate.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">References<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <table class=\"dlw-table\">\n\n                <tbody>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina Viral Surveillance Panel v2 Kit, Set A (96 samples)<\/td>\n\n                        <td>20108081<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina Viral Surveillance Panel v2 Kit, Set B (96 samples)<\/td>\n\n                        <td>20108082<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina Viral Surveillance Panel v2 Kit, Set C (96 samples)<\/td>\n\n                        <td>20108083<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina Viral Surveillance Panel v2 Kit, Set D (96 samples)<\/td>\n\n                        <td>20108084<\/td>\n\n                    <\/tr>\n\n                    <tr>\n\n                        <td class=\"dlw-ref-code\">Illumina Viral Surveillance Panel v2, Panel Only (96 samples)<\/td>\n\n                        <td>20123403<\/td>\n\n                    <\/tr>\n\n                <\/tbody>\n\n            <\/table>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <!-- Modal Structure (Lightbox) -->\n\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n\n    <\/div>\n\n\n\n<\/div>\n\n\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n\n<script>\n\n    function openDlwModal(src) {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        var modalImg = document.getElementById('dlw-modal-img-target');\n\n        modalImg.src = src;\n\n        modal.classList.add('dlw-active');\n\n        document.body.style.overflow = 'hidden'; \/\/ Locks background scroll\n\n    }\n\n\n\n    function closeDlwModal() {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        modal.classList.remove('dlw-active');\n\n        document.body.style.overflow = 'auto'; \/\/ Restores scroll\n\n    }\n\n\n\n    \/\/ Closes modal if clicking outside the image\n\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n\n        if (e.target === this) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n\n\n    \/\/ Closes modal with ESC key\n\n    document.addEventListener('keydown', function(e) {\n\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n<\/script>\n","protected":false},"excerpt":{"rendered":"<p>Next\u2011generation sequencing (NGS) assay with hybrid capture enrichment for the detection and whole\u2011genome sequencing of approximately 200 RNA and DNA viruses relevant to public health. It integrates library preparation, enrichment, sequencing, and data analysis into an optimized workflow of about two days. Detailed Description Principle of operation The panel can start from RNA, DNA, or [&hellip;]<\/p>\n","protected":false},"featured_media":27562,"template":"","tecnologias":[36],"marcas":[232],"familias":[328,335],"class_list":["post-27566","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-illumina-en","familias-microbiology","familias-virus-en"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27566","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27566\/revisions"}],"predecessor-version":[{"id":27567,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27566\/revisions\/27567"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27562"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27566"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27566"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27566"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27566"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}