{"id":27606,"date":"2026-05-22T11:24:38","date_gmt":"2026-05-22T11:24:38","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27606"},"modified":"2026-05-22T11:31:55","modified_gmt":"2026-05-22T11:31:55","slug":"viasure-16s-v1-v4-ngs-solution","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/viasure-16s-v1-v4-ngs-solution\/","title":{"rendered":"VIASURE 16S V1-V4 NGS Solution"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded, and Scaled to 14px) -->\n\n<style>\n\n    \/* Corporate font import *\/\n\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n\n\n    \/* Main Isolated Container *\/\n\n    .dlw-prod-wrapper {\n\n        font-family: 'Raleway', sans-serif !important;\n\n        color: #000000 !important;\n\n        line-height: 1.6 !important;\n\n        background-color: 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==========================================\n\n     UNIFIED AND SHIELDED CONTENT\n\n     ========================================== -->\n\n<div class=\"dlw-prod-wrapper\">\n\n\n\n    <!-- Short Description (Lead Box) -->\n\n    <div class=\"dlw-lead-box\">\n\n        <p>NGS solution for library preparation aimed at the simultaneous identification and profiling of microbial species present in DNA extracted from original samples, through the analysis of the variable regions V1-V4 of the 16S rRNA gene. Product for research use only (RUO) and not intended for diagnostic procedures.<\/p>\n\n    <\/div>\n\n\n\n    <!-- Detailed Description -->\n\n    <h2>Detailed Description<\/h2>\n\n\n\n    <h3>Principle of operation<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">VIASURE 16S V1-V4 NGS Solution is a non\u2011automated device for NGS library preparation based on amplicon enrichment. The assay amplifies target regions of the bacterial 16S rRNA ribosomal gene, specifically the variable domains V1 to V4, and simultaneously adds unique barcodes and indexes necessary for subsequent sequencing.<\/span>\n\n        <span class=\"dlw-list-item\">The workflow is based on a single qPCR reaction that combines the enrichment of target sequences and sample indexing using specifically designed primers. After this reaction, individual libraries are pooled by equal volume or relative quantification, a single final library purification is performed, quantified, diluted, and prepared for sequencing.<\/span>\n\n        <span class=\"dlw-list-item\">Relative quantification of individual libraries is possible thanks to the final fluorescence signal generated by a DNA binding dye included in the reaction mix; this signal is proportional to the amount of target template and can be measured on real\u2011time PCR platforms.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Image 1 (English version) -->\n\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen1-eng-10.webp\" alt=\"Principle of operation VIASURE 16S V1-V4 NGS Solution\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n\n\n    <h3>Applications<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The product is designed to facilitate the identification and profiling of microbial species present in DNA extracted from research samples, by analyzing variable regions of the 16S rRNA gene. 16S rRNA gene sequencing is described as a widely used technique to study the composition and diversity of the microbiome in microbial communities.<\/span>\n\n        <span class=\"dlw-list-item\">Commercial literature indicates that the product supports biological samples or cultures and is aimed at obtaining reliable and accurate results in NGS\u2011based research applications.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Benefits<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Allows coverage of the V1, V2, V3, and V4 regions of the 16S rRNA gene using a OneStep multiplex qPCR strategy.<\/span>\n\n        <span class=\"dlw-list-item\">Integrates target region amplification and barcode incorporation for sequencing into a single qPCR.<\/span>\n\n        <span class=\"dlw-list-item\">Reduces workflow complexity through a simplified process of qPCR, quantification, pooling, and purification.<\/span>\n\n        <span class=\"dlw-list-item\">qPCR allows immediate and accurate quantification of libraries, according to the product leaflet.<\/span>\n\n        <span class=\"dlw-list-item\">Requires only a single final library purification after pooling.<\/span>\n\n        <span class=\"dlw-list-item\">Includes access to Certest bioinformatics software for sequencing data analysis in FASTQ format and generation of Krona reports.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Key results or indicators<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Library size: 500-600 bp.<\/span>\n\n        <span class=\"dlw-list-item\">DNA input requirement: 5 \u00b5l of sample.<\/span>\n\n        <span class=\"dlw-list-item\">Number of pools per panel: 1.<\/span>\n\n        <span class=\"dlw-list-item\">Plexity: up to 48.<\/span>\n\n        <span class=\"dlw-list-item\">Hands\u2011on time: <30 min.<\/span>\n\n        <span class=\"dlw-list-item\">Platforms: Illumina MiSeq and Element Biosciences AVITI.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Technology used<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The assay uses an amplicon\u2011based enrichment NGS library preparation strategy, compatible with Illumina sequencing platforms. The library is generated from isolated microbial DNA, by qPCR for V1-V4 region amplification and sample indexing.<\/span>\n\n    <\/div>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The workflow includes:<\/span>\n\n        <div class=\"dlw-sub-list\">\n\n            <span class=\"dlw-sub-list-item\">Enrichment and indexing.<\/span>\n\n            <span class=\"dlw-sub-list-item\">Pooling.<\/span>\n\n            <span class=\"dlw-sub-list-item\">Final library purification.<\/span>\n\n            <span class=\"dlw-sub-list-item\">Sequencing.<\/span>\n\n            <span class=\"dlw-sub-list-item\">Data analysis.<\/span>\n\n        <\/div>\n\n    <\/div>\n\n\n\n    <h3>Analysis software<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The solution includes access to Certest bioinformatics software for raw sequencing data analysis (V-Xplora). It is a web application developed by Certest Biotec S.L. that allows generating Krona files or reports from FASTQ files.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Image 2 (same as original) -->\n\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen2.webp\" alt=\"Analysis software VIASURE 16S V1-V4 NGS Solution\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n\n\n    <h3>Intended user \/ audience<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The product is intended for qualified laboratory personnel trained in Next Generation Sequencing (NGS) techniques, nucleic acid extraction, sequencer handling, and analysis using pipelines or bioinformatics software.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Considerations or limitations<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Product for research use only (RUO).<\/span>\n\n        <span class=\"dlw-list-item\">Not intended for diagnostic procedures.<\/span>\n\n        <span class=\"dlw-list-item\">No declared clinical purpose and should not be used for diagnosis, prevention, or treatment of diseases.<\/span>\n\n        <span class=\"dlw-list-item\">Product performance characteristics have not been established for diagnostic use.<\/span>\n\n        <span class=\"dlw-list-item\">Some species are better classified by certain variable regions than others; the user must know the regions of interest for classification during bioinformatic analysis.<\/span>\n\n        <span class=\"dlw-list-item\">Because the 16S rRNA gene is highly conserved and widespread among bacteria, environmental contamination may occur if precautions are not strictly followed during the procedure.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Key Features -->\n\n    <h2>Key Features<\/h2>\n\n    \n\n    <div class=\"dlw-check-list\">\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>NGS solution for 16S rRNA gene analysis.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Coverage of variable regions V1, V2, V3, and V4.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Library preparation using OneStep multiplex qPCR.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Enrichment and indexing in a single reaction.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Amplicon\u2011based method.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Compatible with Illumina MiSeq and, according to the leaflet, Element Biosciences AVITI.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Supports biological samples or cultures.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Library size of 500-600 bp.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Up to 48 samples.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Hands\u2011on time &lt;30 min.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Single pool per panel.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Data analysis using V-Xplora \/ VIASURE in\u2011house software.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>RUO product, not intended for diagnosis.<\/span>\n\n        <\/div>\n\n    <\/div>\n\n\n\n    <!-- Presentation Details (Collapsible Accordions) -->\n\n    <h2>Presentation Details<\/h2>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Format and reference<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">Product: VIASURE 16S V1-V4 NGS Solution<\/span>\n\n                <span class=\"dlw-list-item\">Reference: VS-16S-V1448PHRUO<\/span>\n\n                <span class=\"dlw-list-item\">Use: Research Use Only (RUO)<\/span>\n\n                <span class=\"dlw-list-item\">Number of reactions: 48<\/span>\n\n                <span class=\"dlw-list-item\">Product type: non\u2011automated solution for NGS library preparation<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Supplied content<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">MIX 1 16S V14: lyophilized master mix containing primers, DNA binding dye, and components necessary for the generation, enrichment, and indexing of libraries of the target regions. Presentation: 1 plate with 48 wells.<\/span>\n\n                <span class=\"dlw-list-item\">MIX 1 Rehydration buffer: solution to reconstitute the stabilized MIX 1 product. Presentation: 1 vial of 1.8 ml.<\/span>\n\n                <span class=\"dlw-list-item\">Tear-off-8-cap strips: optical caps for sealing during thermal cycling. Presentation: 1 bag with 6 strips of 8 caps.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Transport, storage and handling conditions<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">The product can be transported and stored between 15-30 \u00b0C until the expiration date indicated on the label.<\/span>\n\n                <span class=\"dlw-list-item\">Vibrations during transport must be avoided to prevent leaks.<\/span>\n\n                <span class=\"dlw-list-item\">It is recommended not to rehydrate wells that will not be used.<\/span>\n\n                <span class=\"dlw-list-item\">Unused wells must be kept in the supplied aluminum bags with silica gel, protected from light.<\/span>\n\n                <span class=\"dlw-list-item\">Once wells are rehydrated, nucleic acid must be added and PCR performed immediately.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <!-- Modal Structure (Lightbox) -->\n\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n\n    <\/div>\n\n\n\n<\/div>\n\n\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n\n<script>\n\n    function openDlwModal(src) {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        var modalImg = document.getElementById('dlw-modal-img-target');\n\n        modalImg.src = src;\n\n        modal.classList.add('dlw-active');\n\n        document.body.style.overflow = 'hidden'; \/\/ Locks background scroll\n\n    }\n\n\n\n    function closeDlwModal() {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        modal.classList.remove('dlw-active');\n\n        document.body.style.overflow = 'auto'; \/\/ Restores scroll\n\n    }\n\n\n\n    \/\/ Closes modal if clicking outside the image\n\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n\n        if (e.target === this) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n\n\n    \/\/ Closes modal with ESC key\n\n    document.addEventListener('keydown', function(e) {\n\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n<\/script>\n","protected":false},"excerpt":{"rendered":"<p>NGS solution for library preparation aimed at the simultaneous identification and profiling of microbial species present in DNA extracted from original samples, through the analysis of the variable regions V1-V4 of the 16S rRNA gene. Product for research use only (RUO) and not intended for diagnostic procedures. Detailed Description Principle of operation VIASURE 16S V1-V4 [&hellip;]<\/p>\n","protected":false},"featured_media":27601,"template":"","tecnologias":[36],"marcas":[531],"familias":[328,329],"class_list":["post-27606","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-certest-en","familias-microbiology","familias-bacteria"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27606","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27606\/revisions"}],"predecessor-version":[{"id":27610,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27606\/revisions\/27610"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27601"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27606"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27606"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27606"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27606"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}