{"id":27619,"date":"2026-05-25T07:27:01","date_gmt":"2026-05-25T07:27:01","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=27619"},"modified":"2026-05-25T08:01:29","modified_gmt":"2026-05-25T08:01:29","slug":"sepsis-pathogenic-microorganism-detection-kit-digital-pcr","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/sepsis-pathogenic-microorganism-detection-kit-digital-pcr\/","title":{"rendered":"Sepsis Pathogenic Microorganism Detection Kit (Digital PCR)"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded, and Scaled to 14px) -->\n\n<style>\n\n    \/* Corporate font import *\/\n\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n\n\n    \/* Main Isolated Container *\/\n\n    .dlw-prod-wrapper {\n\n        font-family: 'Raleway', sans-serif !important;\n\n        color: #000000 !important;\n\n        line-height: 1.6 !important;\n\n        background-color: transparent !important;\n\n        padding: 0 !important;\n\n        max-width: 100% !important;\n\n        text-align: left !important;\n\n        display: block !important;\n\n        font-size: 14px !important; \/* ANCHOR: Ignores defective root size from Bricks *\/\n\n    }\n\n\n\n    \/* Local CSS Variables *\/\n\n    .dlw-prod-wrapper {\n\n        --dlw-blue-dark: #003a70;\n\n        --dlw-blue-cyan: #00a1e0;\n\n        --dlw-light-bg: #f9fbfd;\n\n        --dlw-grey-bg: #f4f6f8;\n\n        --dlw-border: #e1e8f0;\n\n        --dlw-grey: #939598;\n\n    }\n\n\n\n    \/* H2 Headings *\/\n\n    .dlw-prod-wrapper h2 {\n\n        font-family: 'Playfair Display', serif !important;\n\n        color: var(--dlw-blue-dark) !important;\n\n        margin-top: 2em !important;\n\n        margin-bottom: 0.8em !important;\n\n        font-size: 1.8em !important;\n\n        font-weight: 700 !important;\n\n        border-bottom: 2px solid var(--dlw-border) !important;\n\n        padding-bottom: 10px !important;\n\n        display: block !important;\n\n    }\n\n\n\n    \/* H3 Headings *\/\n\n    .dlw-prod-wrapper h3 {\n\n        font-family: 'Playfair Display', serif !important;\n\n        color: var(--dlw-blue-cyan) !important;\n\n        margin-top: 1.5em !important;\n\n        margin-bottom: 0.5em !important;\n\n        font-size: 1.4em !important;\n\n        font-weight: 700 !important;\n\n        display: block !important;\n\n    }\n\n\n\n    \/* Standard paragraphs *\/\n\n    .dlw-prod-wrapper p {\n\n        font-weight: 400 !important;\n\n        margin-bottom: 1.2em !important;\n\n        text-align: justify !important;\n\n        font-size: 1.25em !important;\n\n        color: #333333 !important;\n\n    }\n\n\n\n    \/* Corporate bold text *\/\n\n    .dlw-prod-wrapper strong {\n\n        color: var(--dlw-blue-dark) !important;\n\n        font-weight: 700 !important;\n\n    }\n\n\n\n    \/* Lead \/ Short Description (Corporate Blue Box) *\/\n\n    .dlw-lead-box {\n\n        background-color: var(--dlw-blue-dark) !important;\n\n        color: #ffffff !important;\n\n        padding: 2.5em 3em !important;\n\n        margin-bottom: 2.5em !important;\n\n        border-radius: 8px !important;\n\n        box-shadow: 0 6px 25px rgba(0, 58, 112, 0.2) !important;\n\n    }\n\n\n\n    .dlw-lead-box p {\n\n        color: #ffffff !important;\n\n        font-size: 1.35em !important;\n\n        font-weight: 500 !important;\n\n        margin-bottom: 0 !important;\n\n        text-align: left !important;\n\n    }\n\n\n\n    \/* Single inserted images (centered) *\/\n\n    .dlw-prod-img {\n\n        width: auto !important;\n\n        max-width: 90% !important;\n\n        height: auto !important;\n\n        display: block !important;\n\n        margin: 2.5em auto !important;\n\n        margin-left: auto !important;\n\n        margin-right: auto !important;\n\n        float: none !important;\n\n        border-radius: 6px !important;\n\n        box-shadow: none !important;\n\n        transition: transform 0.4s cubic-bezier(0.165, 0.84, 0.44, 1) !important;\n\n        cursor: zoom-in !important;\n\n    }\n\n\n\n    .dlw-prod-img:hover {\n\n        transform: scale(1.03) !important;\n\n    }\n\n\n\n    \/* Standard lists (bullets) *\/\n\n    .dlw-list {\n\n        display: flex !important;\n\n        flex-direction: column !important;\n\n        margin-bottom: 2em !important;\n\n        padding-left: 0 !important;\n\n    }\n\n\n\n    .dlw-list-item {\n\n        position: relative !important;\n\n        padding-left: 1.5em !important;\n\n        margin-bottom: 0.8em !important;\n\n        text-align: left !important;\n\n        font-size: 1.25em !important;\n\n        color: #333333 !important;\n\n        display: block !important;\n\n    }\n\n\n\n    .dlw-list-item::before {\n\n        content: \"\u2022\" !important;\n\n        position: absolute !important;\n\n        left: 0 !important;\n\n        color: var(--dlw-blue-cyan) !important;\n\n        font-weight: bold !important;\n\n        font-size: 1.2em !important;\n\n        top: -2px !important;\n\n    }\n\n\n\n    \/* Nested sub-lists *\/\n\n    .dlw-sub-list {\n\n        margin-top: 0.5em !important;\n\n        margin-bottom: 0.5em !important;\n\n        padding-left: 1.5em !important;\n\n    }\n\n\n\n    .dlw-sub-list-item {\n\n        position: relative !important;\n\n        padding-left: 1.2em !important;\n\n        margin-bottom: 0.4em !important;\n\n        font-size: 0.95em !important;\n\n        color: #555555 !important;\n\n        display: block !important;\n\n    }\n\n\n\n    .dlw-sub-list-item::before {\n\n        content: \"-\" !important;\n\n        position: absolute !important;\n\n        left: 0 !important;\n\n        color: var(--dlw-grey) !important;\n\n        font-weight: bold !important;\n\n    }\n\n\n\n    \/* Check lists (Key Features) *\/\n\n    .dlw-check-list {\n\n        display: grid !important;\n\n        grid-template-columns: 1fr 1fr !important;\n\n        gap: 1.5em 2em !important;\n\n        margin: 2em 0 !important;\n\n        background-color: var(--dlw-light-bg) !important;\n\n        padding: 2.5em !important;\n\n        border-radius: 8px !important;\n\n        border-left: 6px solid var(--dlw-blue-cyan) !important;\n\n    }\n\n\n\n    .dlw-check-item {\n\n        display: flex !important;\n\n        align-items: flex-start !important;\n\n        gap: 12px !important;\n\n        font-size: 1.2em !important;\n\n        color: #333333 !important;\n\n        line-height: 1.4 !important;\n\n    }\n\n\n\n    .dlw-check-icon {\n\n        width: 24px !important;\n\n        height: 24px !important;\n\n        min-width: 24px !important;\n\n        fill: var(--dlw-blue-cyan) !important;\n\n        margin-top: 2px !important;\n\n        flex-shrink: 0 !important;\n\n    }\n\n    \n\n    .dlw-check-item:last-child:nth-child(odd) {\n\n        grid-column: 1 \/ -1 !important;\n\n    }\n\n\n\n    \/* Corporate tables (for references and microorganism panel) *\/\n\n    .dlw-table {\n\n        width: 100% !important;\n\n        border-collapse: collapse !important;\n\n        margin-bottom: 2.5em !important;\n\n        background-color: #ffffff !important;\n\n        box-shadow: 0 2px 10px rgba(0,0,0,0.03) !important;\n\n        border-radius: 6px !important;\n\n        overflow: hidden !important;\n\n    }\n\n\n\n    .dlw-table th {\n\n        background-color: var(--dlw-blue-dark) !important;\n\n        color: #ffffff !important;\n\n        text-align: center !important;\n\n        padding: 15px 20px !important;\n\n        font-family: 'Raleway', sans-serif !important;\n\n        font-weight: 600 !important;\n\n        text-transform: uppercase !important;\n\n        font-size: 1.1em !important;\n\n        letter-spacing: 0.5px !important;\n\n    }\n\n\n\n    .dlw-table td {\n\n        padding: 20px !important;\n\n        border-bottom: 1px solid var(--dlw-border) !important;\n\n        vertical-align: top !important;\n\n        font-size: 1.15em !important;\n\n        color: #333333 !important;\n\n        background-color: #ffffff !important;\n\n    }\n\n\n\n    .dlw-table tr:last-child td {\n\n        border-bottom: 2px solid var(--dlw-blue-cyan) !important;\n\n    }\n\n\n\n    \/* Specific list style inside the table *\/\n\n    .microbe-list {\n\n        list-style: none !important;\n\n        margin: 0 !important;\n\n        padding-left: 1.2em !important;\n\n    }\n\n    .microbe-list li {\n\n        position: relative !important;\n\n        margin-bottom: 0.6em !important;\n\n        line-height: 1.4 !important;\n\n    }\n\n    .microbe-list li::before {\n\n        content: \"\u2022\" !important;\n\n        position: absolute !important;\n\n        left: -1.2em !important;\n\n        color: var(--dlw-blue-cyan) !important;\n\n        font-weight: bold !important;\n\n        font-size: 1.1em !important;\n\n    }\n\n    .panel-note {\n\n        margin-top: 1.2em !important;\n\n        font-style: italic !important;\n\n        color: var(--dlw-blue-dark) !important;\n\n        font-size: 0.95em !important;\n\n    }\n\n\n\n    .dlw-ref-code {\n\n        font-family: 'Courier New', Courier, monospace !important;\n\n        font-weight: 700 !important;\n\n        color: var(--dlw-blue-dark) !important;\n\n    }\n\n\n\n    \/* Accordions *\/\n\n    .dlw-accordion {\n\n        background-color: #ffffff !important;\n\n        border: 1px solid var(--dlw-border) !important;\n\n        border-radius: 6px !important;\n\n        margin-bottom: 1.5em !important;\n\n        overflow: hidden !important;\n\n        transition: all 0.3s ease !important;\n\n    }\n\n\n\n    .dlw-accordion-header {\n\n        background-color: var(--dlw-blue-dark) !important;\n\n        color: #ffffff !important;\n\n        padding: 15px 20px !important;\n\n        font-family: 'Raleway', sans-serif !important;\n\n        font-weight: 600 !important;\n\n        text-transform: uppercase !important;\n\n        font-size: 1.1em !important;\n\n        letter-spacing: 0.5px !important;\n\n        cursor: pointer !important;\n\n        list-style: none !important;\n\n        position: relative !important;\n\n        display: flex !important;\n\n        justify-content: space-between !important;\n\n        align-items: center !important;\n\n        transition: background-color 0.3s ease !important;\n\n    }\n\n\n\n    .dlw-accordion-header::-webkit-details-marker {\n\n        display: none !important;\n\n    }\n\n\n\n    .dlw-accordion-header::after {\n\n        content: '+' !important;\n\n        font-size: 1.8em !important;\n\n        line-height: 1 !important;\n\n        color: var(--dlw-blue-cyan) !important;\n\n        transition: transform 0.3s ease !important;\n\n    }\n\n\n\n    .dlw-accordion[open] .dlw-accordion-header::after {\n\n        transform: rotate(45deg) !important;\n\n    }\n\n\n\n    .dlw-accordion[open] .dlw-accordion-header {\n\n        border-bottom: 2px solid var(--dlw-blue-cyan) !important;\n\n    }\n\n\n\n    .dlw-accordion-content {\n\n        padding: 20px !important;\n\n        background-color: #ffffff !important;\n\n    }\n\n\n\n    .dlw-accordion-content .dlw-table {\n\n        margin-bottom: 0 !important;\n\n        box-shadow: none !important;\n\n        border-radius: 0 !important;\n\n        border-top: none !important;\n\n    }\n\n\n\n    .dlw-accordion-content .dlw-list {\n\n        margin-bottom: 0 !important;\n\n    }\n\n    \n\n    .dlw-accordion-content p {\n\n        margin-bottom: 0 !important;\n\n    }\n\n\n\n    \/* Modal \/ Lightbox *\/\n\n    .dlw-modal {\n\n        display: none;\n\n        position: fixed !important;\n\n        z-index: 999999 !important;\n\n        left: 0 !important;\n\n        top: 0 !important;\n\n        width: 100vw !important;\n\n        height: 100vh !important;\n\n        background-color: rgba(0, 20, 40, 0.85) !important;\n\n        backdrop-filter: blur(5px) !important;\n\n        align-items: center !important;\n\n        justify-content: center !important;\n\n    }\n\n\n\n    .dlw-modal.dlw-active {\n\n        display: flex !important;\n\n        animation: dlw-fade-in 0.3s ease-out forwards;\n\n    }\n\n\n\n    @keyframes dlw-fade-in {\n\n        from { opacity: 0; }\n\n        to { opacity: 1; }\n\n    }\n\n\n\n    .dlw-modal-img {\n\n        max-width: 90vw !important;\n\n        max-height: 90vh !important;\n\n        width: auto !important;\n\n        height: auto !important;\n\n        border-radius: 6px !important;\n\n        box-shadow: 0 10px 40px rgba(0,0,0,0.5) !important;\n\n        object-fit: contain !important;\n\n    }\n\n\n\n    .dlw-modal-close {\n\n        position: absolute !important;\n\n        top: 25px !important;\n\n        right: 35px !important;\n\n        color: #ffffff !important;\n\n        font-size: 45px !important;\n\n        font-weight: 300 !important;\n\n        cursor: pointer !important;\n\n        transition: color 0.3s ease !important;\n\n        line-height: 1 !important;\n\n        z-index: 9999999 !important;\n\n    }\n\n\n\n    .dlw-modal-close:hover {\n\n        color: var(--dlw-blue-cyan) !important;\n\n    }\n\n\n\n    \/* Responsive *\/\n\n    @media (max-width: 768px) {\n\n        .dlw-lead-box { padding: 1.8em !important; }\n\n        .dlw-check-list { grid-template-columns: 1fr !important; padding: 1.5em !important; }\n\n        .dlw-prod-img { max-width: 100% !important; }\n\n        \n\n        \/* Responsive tables *\/\n\n        .dlw-table, .dlw-table thead, .dlw-table tbody, .dlw-table th, .dlw-table td, .dlw-table tr { display: block !important; }\n\n        .dlw-table thead tr { position: absolute !important; top: -9999px !important; left: -9999px !important; }\n\n        .dlw-table tr { border-bottom: 2px solid var(--dlw-blue-cyan) !important; margin-bottom: 15px !important; }\n\n        .dlw-table td { border: none !important; position: relative !important; padding-left: 45% !important; }\n\n        .dlw-table td::before { position: absolute !important; top: 15px !important; left: 15px !important; width: 40% !important; white-space: nowrap !important; font-weight: bold !important; color: var(--dlw-blue-dark) !important; font-size: 0.9em !important; text-transform: uppercase !important; }\n\n        .dlw-table td:nth-of-type(1)::before { content: \"Panel 1\" !important; }\n\n        .dlw-table td:nth-of-type(2)::before { content: \"Panel 2\" !important; }\n\n    }\n\n<\/style>\n\n\n\n<!-- ==========================================\n\n     UNIFIED AND SHIELDED CONTENT\n\n     ========================================== -->\n\n<div class=\"dlw-prod-wrapper\">\n\n\n\n    <!-- Short Description (Lead Box) -->\n\n    <div class=\"dlw-lead-box\">\n\n        <p>In vitro diagnostic kit based on droplet digital PCR (dPCR) for the detection of pathogenic microorganisms associated with sepsis from DNA extracted from clinical samples. The kit allows identification and quantification of pathogens without requiring blood culture, covering 21 frequent agents in bloodstream infections.<\/p>\n\n    <\/div>\n\n\n\n    <!-- Detailed Description -->\n\n    <h2>Detailed Description<\/h2>\n\n\n\n    <h3>Principle of operation<\/h3>\n\n    <p>The <strong>Sepsis Pathogenic Microorganism Detection Kit (Digital PCR)<\/strong> uses <strong>droplet digital PCR<\/strong> technology combined with <strong>TaqMan<\/strong> fluorescent probes to detect specific gene fragments of different pathogenic microorganisms. Each target is identified by one\u2011 or two\u2011color fluorescent probes and, by combining probes in different proportions, the system allows simultaneous detection of multiple pathogens in a single well.<\/p>\n\n    <p>During the procedure, microbial DNA extracted from the sample is randomly distributed into thousands of oil\u2011encapsulated droplets inside the digital PCR cartridge. After amplification, the fluorescent signals from each droplet are analyzed using statistical methods based on Poisson distribution, allowing precise detection of the microorganisms present.<\/p>\n\n    <p>The assay is organized into two main mixtures or panels, <strong>Sepsis Buffer 1 and Sepsis Buffer 2<\/strong>, which are processed separately for each sample and for the controls. Each reaction has a final volume of <strong>20 \u00b5l<\/strong> and includes enzyme mix, specific buffer, nuclease\u2011free water, and sample or control DNA.<\/p>\n\n\n\n    <h3>Clinical applications<\/h3>\n\n    <p>The kit is designed for the detection of various pathogens involved in sepsis, a life\u2011threatening condition caused by microbial invasion of the bloodstream. The documentation indicates that bloodstream infection is a precursor to sepsis and can be caused by bacteria, viruses, and fungi. The product allows identification of types and loads of pathogenic microorganisms in blood without the need for blood culture. <strong>21 frequent pathogenic agents in bloodstream infections<\/strong>, including Gram\u2011negative bacteria, Gram\u2011positive bacteria, and fungi.<\/p>\n\n\n\n    <!-- Image 1 (English version) -->\n\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/05\/Imagen1-eng-11.webp\" alt=\"Sepsis digital PCR kit workflow diagram\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n\n\n    <h3>Detected microorganisms<\/h3>\n\n    <p>The kit detects the following pathogens included in the two CE\u2011IVD panels:<\/p>\n\n    <table class=\"dlw-table\">\n\n        <thead>\n\n            <tr>\n\n                <th>Panel 1<\/th>\n\n                <th>Panel 2<\/th>\n\n            <\/tr>\n\n        <\/thead>\n\n        <tbody>\n\n            <tr>\n\n                <td>\n\n                    <ul class=\"microbe-list\">\n\n                        <li>Staphylococcus capitis<\/li>\n\n                        <li>Acinetobacter baumannii<\/li>\n\n                        <li>Candida tropicalis<\/li>\n\n                        <li>Candida krusei<\/li>\n\n                        <li>Staphylococcus epidermidis<\/li>\n\n                        <li>Enterococcus faecium<\/li>\n\n                        <li>Cryptococcus neoformans<\/li>\n\n                        <li>Stenotrophomonas maltophilia<\/li>\n\n                        <li>Klebsiella pneumoniae<\/li>\n\n                        <li>Streptococcus pneumoniae<\/li>\n\n                        <li>Serratia marcescens<\/li>\n\n                    <\/ul>\n\n                <\/td>\n\n                <td>\n\n                    <ul class=\"microbe-list\">\n\n                        <li>Bacteroides fragilis<\/li>\n\n                        <li>Escherichia coli<\/li>\n\n                        <li>Candida parapsilosis<\/li>\n\n                        <li>Candida glabrata<\/li>\n\n                        <li>Enterobacter cloacae complex<\/li>\n\n                        <li>Enterococcus faecalis<\/li>\n\n                        <li>Haemophilus influenzae<\/li>\n\n                        <li>Pseudomonas aeruginosa<\/li>\n\n                        <li>Staphylococcus aureus<\/li>\n\n                        <li>Candida albicans<\/li>\n\n                    <\/ul>\n\n                    <div class=\"panel-note\">Panel 2 also includes an internal control.<\/div>\n\n                <\/td>\n\n            <\/tr>\n\n        <\/tbody>\n\n    <\/table>\n\n\n\n    <h3>Benefits<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Allows direct molecular detection of sepsis\u2011associated pathogens by digital PCR, without relying on blood culture growth.<\/span>\n\n        <span class=\"dlw-list-item\">Provides rapid and accurate identification of microbial nucleic acids in whole blood, aimed at facilitating faster and targeted treatment.<\/span>\n\n        <span class=\"dlw-list-item\">The RainSure workflow allows working with 3 ml of blood and obtaining results in an average of 3\u20115 hours.<\/span>\n\n        <span class=\"dlw-list-item\">Offers absolute quantification, high sensitivity, and rapid detection, according to the technological comparison included in the technical documentation.<\/span>\n\n        <span class=\"dlw-list-item\">Allows detection of co\u2011infections and multiple pathogens in the same sample, according to the product technical description.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Key results or indicators<\/h3>\n\n    <p>Interpretation of results is based on the count of positive droplets relative to the Limit of Blank (LoB) defined for each target. For all pathogens included in the panels, the indicated positive value is \u22653 positive droplets.<\/p>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">If the number of positive droplets for a target exceeds the LoB, the sample is reported as positive for that pathogen.<\/span>\n\n        <span class=\"dlw-list-item\">If the number of positive droplets is below the LoB, the sample is reported as negative.<\/span>\n\n        <span class=\"dlw-list-item\">If the number of positive droplets equals the LoB, it is recommended to repeat the assay with the maximum sample volume; if the repeat exceeds the LoB, the pathogen is reported as positive.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Technology used<\/h3>\n\n    <p>The product is based on droplet digital PCR with multi\u2011channel fluorescent detection. The compatible system indicated in the IFU is the RainSure DropX\u20112250 series digital PCR system, with five fluorescence channels: FAM, HEX, ROX, Cy5, and Cy5.5.<\/p>\n\n\n\n    <h3>Intended user \/ audience<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Qualified laboratory personnel trained in dPCR techniques.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Considerations or limitations<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">The kit is intended for in vitro diagnostic (IVD) use.<\/span>\n\n        <span class=\"dlw-list-item\">Results may be affected by sample collection, handling, transport, and storage.<\/span>\n\n        <span class=\"dlw-list-item\">Cross\u2011contamination can produce false positives.<\/span>\n\n        <span class=\"dlw-list-item\">Inadequate collection, transport, or handling can generate false negatives.<\/span>\n\n        <span class=\"dlw-list-item\">Variations in the analyzed target sequences can also cause false negatives.<\/span>\n\n        <span class=\"dlw-list-item\">Use is restricted to the indicated sample types and analysis systems, including instrumental models, nucleic acid extraction reagents, and applicable methods.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Key Features -->\n\n    <h2>Key Features<\/h2>\n\n    \n\n    <div class=\"dlw-check-list\">\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>IVD kit for detection of sepsis\u2011associated pathogens.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Droplet digital PCR technology with TaqMan probes.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Detection of 21 frequent microorganisms in bloodstream infections.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Includes Gram\u2011positive bacteria, Gram\u2011negative bacteria, and fungi.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Allows detection without blood culture.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Compatible with RainSure DropX\u20112250 system.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Multi\u2011channel analysis: FAM, HEX, ROX, Cy5, and Cy5.5.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Interpretation based on positive droplets and LoB \u22653.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Average time indicated in technical literature: 3\u20115 hours.<\/span>\n\n        <\/div>\n\n        <div class=\"dlw-check-item\">\n\n            <svg class=\"dlw-check-icon\" viewBox=\"0 0 24 24\"><path d=\"M12 2C6.48 2 2 6.48 2 12s4.48 10 10 10 10-4.48 10-10S17.52 2 12 2zm-2 15l-5-5 1.41-1.41L10 14.17l7.59-7.59L19 8l-9 9z\"\/><\/svg>\n\n            <span>Allows absolute quantification and detection of multiple pathogens in the same sample.<\/span>\n\n        <\/div>\n\n    <\/div>\n\n\n\n    <!-- Presentation Details (Collapsible Accordions) -->\n\n    <h2>Presentation Details<\/h2>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Format and reference<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">Product: Sepsis Pathogenic Microorganism Detection Kit (Digital PCR)<\/span>\n\n                <span class=\"dlw-list-item\">Reference: 3.02.03.0007<\/span>\n\n                <span class=\"dlw-list-item\">Presentation: 24 tests\/kit<\/span>\n\n                <span class=\"dlw-list-item\">Use: In vitro diagnostic (IVD)<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Kit components<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">Sepsis Buffer 1: 1 vial of 210 \u00b5l. Contains dNTPs, MgCl\u2082, KCl, Tris\u2011HCl, and primers\/probes for Panel 1 pathogens.<\/span>\n\n                <span class=\"dlw-list-item\">Sepsis Buffer 2: 1 vial of 210 \u00b5l. Contains dNTPs, MgCl\u2082, KCl, Tris\u2011HCl, primers\/probes for Panel 2 pathogens, and internal control primers\/probes.<\/span>\n\n                <span class=\"dlw-list-item\">Enzyme Mix: 1 vial of 60 \u00b5l, with Taq DNA polymerase.<\/span>\n\n                <span class=\"dlw-list-item\">Sepsis Positive Control: 1 vial of 160 \u00b5l, with plasmids corresponding to the kit targets and internal control.<\/span>\n\n                <span class=\"dlw-list-item\">Sepsis Negative Control: 1 vial of 160 \u00b5l, DNase\/RNase\u2011free water.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Storage conditions<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">Store protected from light at a temperature below -15 \u00b0C.<\/span>\n\n                <span class=\"dlw-list-item\">Shelf life: 12 months.<\/span>\n\n                <span class=\"dlw-list-item\">Avoid more than 7 freeze\/thaw cycles.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Sample types<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">The IFU indicates the following sample types:<\/span>\n\n                <div class=\"dlw-sub-list\">\n\n                    <span class=\"dlw-sub-list-item\">Peripheral blood with EDTA anticoagulant.<\/span>\n\n                    <span class=\"dlw-sub-list-item\">Sputum.<\/span>\n\n                    <span class=\"dlw-sub-list-item\">Bronchoalveolar lavage.<\/span>\n\n                    <span class=\"dlw-sub-list-item\">Cerebrospinal fluid.<\/span>\n\n                    <span class=\"dlw-sub-list-item\">Pleural and abdominal fluid.<\/span>\n\n                    <span class=\"dlw-sub-list-item\">Urine.<\/span>\n\n                    <span class=\"dlw-sub-list-item\">Pus.<\/span>\n\n                <\/div>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <details class=\"dlw-accordion\">\n\n        <summary class=\"dlw-accordion-header\">Required materials not included<\/summary>\n\n        <div class=\"dlw-accordion-content\">\n\n            <div class=\"dlw-list\">\n\n                <span class=\"dlw-list-item\">RainSure Droplet Generation Oil for probes.<\/span>\n\n                <span class=\"dlw-list-item\">RainSure Digital PCR Cartridge.<\/span>\n\n                <span class=\"dlw-list-item\">RainSure Sealing Covers.<\/span>\n\n                <span class=\"dlw-list-item\">Nuclease\u2011free water.<\/span>\n\n                <span class=\"dlw-list-item\">Filter tips.<\/span>\n\n                <span class=\"dlw-list-item\">Low\u2011binding, nuclease\u2011free centrifuge tubes.<\/span>\n\n                <span class=\"dlw-list-item\">Pipette, centrifuge, and vortex.<\/span>\n\n            <\/div>\n\n        <\/div>\n\n    <\/details>\n\n\n\n    <!-- Modal Structure (Lightbox) -->\n\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n\n    <\/div>\n\n\n\n<\/div>\n\n\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n\n<script>\n\n    function openDlwModal(src) {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        var modalImg = document.getElementById('dlw-modal-img-target');\n\n        modalImg.src = src;\n\n        modal.classList.add('dlw-active');\n\n        document.body.style.overflow = 'hidden';\n\n    }\n\n\n\n    function closeDlwModal() {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        modal.classList.remove('dlw-active');\n\n        document.body.style.overflow = 'auto';\n\n    }\n\n\n\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n\n        if (e.target === this) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n\n\n    document.addEventListener('keydown', function(e) {\n\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n<\/script>\n","protected":false},"excerpt":{"rendered":"<p>In vitro diagnostic kit based on droplet digital PCR (dPCR) for the detection of pathogenic microorganisms associated with sepsis from DNA extracted from clinical samples. The kit allows identification and quantification of pathogens without requiring blood culture, covering 21 frequent agents in bloodstream infections. Detailed Description Principle of operation The Sepsis Pathogenic Microorganism Detection Kit [&hellip;]<\/p>\n","protected":false},"featured_media":27617,"template":"","tecnologias":[123],"marcas":[533],"familias":[328,330],"class_list":["post-27619","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-digital-pcr","marcas-rainsure-en","familias-microbiology","familias-cmi-quick-sepsis"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27619","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27619\/revisions"}],"predecessor-version":[{"id":27621,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/27619\/revisions\/27621"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/27617"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=27619"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=27619"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=27619"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=27619"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}