{"id":28022,"date":"2026-07-22T07:43:06","date_gmt":"2026-07-22T07:43:06","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=28022"},"modified":"2026-07-22T07:45:21","modified_gmt":"2026-07-22T07:45:21","slug":"human-whole-genome","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/human-whole-genome\/","title":{"rendered":"Human-Whole Genome"},"content":{"rendered":"\n<!-- Scoped Styles Block (Isolated, Shielded, and Scaled to 14px) -->\n\n<style>\n\n    \/* Corporate font import *\/\n\n    @import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:ital,wght@0,400;0,700;1,400&family=Raleway:wght@300;400;500;600;700&display=swap');\n\n\n\n    \/* Main Isolated Container *\/\n\n    .dlw-prod-wrapper {\n\n        font-family: 'Raleway', sans-serif !important;\n\n        color: #000000 !important;\n\n        line-height: 1.6 !important;\n\n        background-color: transparent !important;\n\n        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whole\u2011genome sequencing that provides a thorough and high\u2011resolution view, optimising variant detection and genetic research. Its workflow enables the generation of high\u2011precision libraries with uniform coverage.<\/p>\n\n    <\/div>\n\n\n\n    <!-- Detailed description -->\n\n    <h2>Detailed description<\/h2>\n\n\n\n    <h3>Key features<\/h3>\n\n    <p>Illumina DNA PCR-Free Prep, Tagmentation is designed to offer a fast, flexible, and high\u2011throughput workflow for whole\u2011genome sequencing applications. The solution combines bead\u2011based tagmentation technology with PCR\u2011free chemistry, reducing amplification bias and improving coverage uniformity in complex genomic regions or those with irregular base composition.<\/p>\n\n    <p>The protocol is compatible with different input types, allowing the assay to be adapted to various sample types and experimental needs.<\/p>\n\n\n\n    <!-- IMAGE 1 (English version) -->\n\n    <img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/07\/1EN.webp\" alt=\"Illumina DNA PCR-Free Prep, Tagmentation\" class=\"dlw-prod-img\" onclick=\"openDlwModal(this.src)\" title=\"\">\n\n\n\n    <h3>Clinical applications<\/h3>\n\n    <p>Illumina DNA PCR-Free Prep, Tagmentation is primarily aimed at whole\u2011genome sequencing applications, especially when high coverage uniformity and variant calling accuracy are required. Possible applications include, for example:<\/p>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Research into genetic\u2011based rare diseases<\/span>\n\n        <span class=\"dlw-list-item\">Identification of variants in tumour vs. normal samples<\/span>\n\n        <span class=\"dlw-list-item\">De novo microbial genome assembly<\/span>\n\n        <span class=\"dlw-list-item\">Sensitive applications where PCR bias may compromise coverage or accuracy<\/span>\n\n        <span class=\"dlw-list-item\">Library preparation from samples such as blood, saliva, or dried blood spots<\/span>\n\n    <\/div>\n\n\n\n    <h3>Benefits<\/h3>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Higher data quality and uniformity, thanks to a PCR\u2011free workflow combined with bead\u2011based tagmentation, which improves genomic coverage.<\/span>\n\n        <span class=\"dlw-list-item\">Reduction of amplification bias, especially in complex regions such as GC\u2011 or AT\u2011rich areas, repetitive regions, or hard\u2011to\u2011cover areas.<\/span>\n\n        <span class=\"dlw-list-item\">Simpler and faster protocol, with fewer preparation steps and shorter processing time (up to ~90 min for library preparation).<\/span>\n\n        <span class=\"dlw-list-item\">Reduced manual handling, by incorporating automatic normalisation and volume\u2011based library pooling.<\/span>\n\n        <span class=\"dlw-list-item\">More efficient sample processing, reducing the need for quantifications before and after library preparation.<\/span>\n\n        <span class=\"dlw-list-item\">Greater compatibility with automation, facilitating more robust, reproducible and scalable workflows.<\/span>\n\n    <\/div>\n\n\n\n    <h3>Intended user \/ audience<\/h3>\n\n    <p>Solution aimed at genomic research laboratories that require fast, robust and scalable NGS library preparation for whole\u2011genome sequencing and other sensitive applications. It is especially indicated for environments that need to reduce PCR bias, work with low DNA inputs, automate library preparation workflows and process sample batches efficiently.<\/p>\n\n\n\n    <!-- Key features -->\n\n    <h2>Key features<\/h2>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">PCR\u2011free NGS library preparation using bead\u2011based tagmentation.<\/span>\n\n        <span class=\"dlw-list-item\">Fast workflow: approximately 90 minutes from extracted genomic DNA.<\/span>\n\n        <span class=\"dlw-list-item\">Compatible with gDNA, blood, saliva, plasmids and dried blood spots.<\/span>\n\n        <span class=\"dlw-list-item\">Input DNA amount from 25 ng to 300 ng, with a maximum indicated of 2 \u00b5g.<\/span>\n\n        <span class=\"dlw-list-item\">Reduction of PCR\u2011induced bias.<\/span>\n\n        <span class=\"dlw-list-item\">Uniform coverage in human WGS applications.<\/span>\n\n        <span class=\"dlw-list-item\">Improved coverage in complex, GC\u2011 or AT\u2011rich regions.<\/span>\n\n        <span class=\"dlw-list-item\">Compatible with automation.<\/span>\n\n        <span class=\"dlw-list-item\">Up to 384 dual indexes for high\u2011productivity projects.<\/span>\n\n    <\/div>\n\n\n\n    <!-- Presentation details -->\n\n    <h2>Presentation details<\/h2>\n\n    <div class=\"dlw-list\">\n\n        <span class=\"dlw-list-item\">Illumina DNA PCR-Free Prep, Tagmentation (24 samples) \u2014 Catalog number: 20041794<\/span>\n\n        <span class=\"dlw-list-item\">Illumina DNA PCR-Free Prep, Tagmentation (96 samples) \u2014 Catalog number: 20041795<\/span>\n\n        <span class=\"dlw-list-item\">Illumina DNA\/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) \u2014 Catalog number: 20091654<\/span>\n\n        <span class=\"dlw-list-item\">Illumina DNA\/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) \u2014 Catalog number: 20091656<\/span>\n\n        <span class=\"dlw-list-item\">Illumina DNA\/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) \u2014 Catalog number: 20091658<\/span>\n\n        <span class=\"dlw-list-item\">Illumina DNA\/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) \u2014 Catalog number: 20091660<\/span>\n\n        <span class=\"dlw-list-item\">Illumina Lysis Reagent Kit \u2014 Catalog number: 20042221<\/span>\n\n    <\/div>\n\n\n\n    <!-- Modal Structure (Lightbox) -->\n\n    <div id=\"dlw-image-modal\" class=\"dlw-modal\">\n\n        <span class=\"dlw-modal-close\" onclick=\"closeDlwModal()\">&times;<\/span>\n\n        <img decoding=\"async\" class=\"dlw-modal-img\" id=\"dlw-modal-img-target\" src=\"\" alt=\"\" title=\"\">\n\n    <\/div>\n\n\n\n<\/div>\n\n\n\n<!-- Modal Script (Vanilla JS, no dependencies) -->\n\n<script>\n\n    function openDlwModal(src) {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        var modalImg = document.getElementById('dlw-modal-img-target');\n\n        modalImg.src = src;\n\n        modal.classList.add('dlw-active');\n\n        document.body.style.overflow = 'hidden';\n\n    }\n\n\n\n    function closeDlwModal() {\n\n        var modal = document.getElementById('dlw-image-modal');\n\n        modal.classList.remove('dlw-active');\n\n        document.body.style.overflow = 'auto';\n\n    }\n\n\n\n    document.getElementById('dlw-image-modal').addEventListener('click', function(e) {\n\n        if (e.target === this) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n\n\n    document.addEventListener('keydown', function(e) {\n\n        if (e.key === \"Escape\" && document.getElementById('dlw-image-modal').classList.contains('dlw-active')) {\n\n            closeDlwModal();\n\n        }\n\n    });\n\n<\/script>\n","protected":false},"excerpt":{"rendered":"<p>This is a comprehensive solution for human whole\u2011genome sequencing that provides a thorough and high\u2011resolution view, optimising variant detection and genetic research. Its workflow enables the generation of high\u2011precision libraries with uniform coverage. Detailed description Key features Illumina DNA PCR-Free Prep, Tagmentation is designed to offer a fast, flexible, and high\u2011throughput workflow for whole\u2011genome sequencing [&hellip;]<\/p>\n","protected":false},"featured_media":28017,"template":"","tecnologias":[36],"marcas":[232],"familias":[283,426,425],"class_list":["post-28022","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-illumina-en","familias-molecular-genetics","familias-genomes","familias-hereditary_and_or_rare_diseases"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28022","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28022\/revisions"}],"predecessor-version":[{"id":28023,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28022\/revisions\/28023"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/28017"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=28022"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=28022"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=28022"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=28022"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}