{"id":28370,"date":"2026-09-02T11:51:05","date_gmt":"2026-09-02T11:51:05","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=28370"},"modified":"2026-09-02T11:54:16","modified_gmt":"2026-09-02T11:54:16","slug":"duplic%ce%b1-realtime-apoe-genotyping-kit","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/duplic%ce%b1-realtime-apoe-genotyping-kit\/","title":{"rendered":"Duplic\u03b1 RealTime ApoE Genotyping Kit"},"content":{"rendered":"\n<!-- Complete HTML ready for WordPress \/ Bricks -->\n\n<style>\n    .dlw-prod-wrapper {\n        --dlw-blue-dark: #003a70;\n        --dlw-blue-cyan: #00a1e0;\n        --dlw-light-bg: #f9fbfd;\n        --dlw-grey-bg: #f4f6f8;\n        --dlw-border: #e1e8f0;\n\n        width: 100% !important;\n        max-width: 720px !important;\n        margin: 0 auto !important;\n        padding: 0 !important;\n        overflow-wrap: break-word;\n        box-sizing: border-box !important;\n        color: #333333;\n        font-family: \"Raleway\", Arial, sans-serif;\n        font-size: 16px;\n        line-height: 1.75;\n    }\n\n    .dlw-prod-wrapper *,\n    .dlw-prod-wrapper *::before,\n    .dlw-prod-wrapper *::after {\n        box-sizing: border-box !important;\n    }\n\n    .dlw-prod-wrapper .dlw-intro {\n        margin: 0 0 30px !important;\n        padding: 22px 24px !important;\n        border-left: 4px solid var(--dlw-blue-cyan);\n        background-color: var(--dlw-light-bg);\n        text-align: justify;\n    }\n\n    .dlw-prod-wrapper h2.dlw-section-title {\n        position: relative;\n        margin: 42px 0 24px !important;\n        padding: 0 0 13px !important;\n        color: var(--dlw-blue-dark) !important;\n        font-family: \"Playfair Display\", Georgia, serif !important;\n        font-size: 28px !important;\n        font-weight: 500 !important;\n        line-height: 1.25 !important;\n    }\n\n    .dlw-prod-wrapper h2.dlw-section-title::after {\n        position: absolute;\n        bottom: 0;\n        left: 0;\n        width: 52px;\n        height: 3px;\n        background-color: var(--dlw-blue-cyan);\n        content: \"\";\n    }\n\n    .dlw-prod-wrapper h3.dlw-subsection-title {\n        margin: 28px 0 14px !important;\n        color: var(--dlw-blue-cyan) !important;\n        font-family: \"Playfair Display\", Georgia, serif !important;\n        font-size: 22px !important;\n        font-weight: 600 !important;\n        line-height: 1.3 !important;\n    }\n\n    .dlw-prod-wrapper h4.dlw-detail-title {\n        margin: 25px 0 12px !important;\n        color: var(--dlw-blue-dark) !important;\n        font-family: \"Playfair Display\", Georgia, serif !important;\n        font-size: 18px !important;\n        font-weight: 700 !important;\n        line-height: 1.35 !important;\n    }\n\n    .dlw-prod-wrapper p.dlw-text {\n        margin: 0 0 18px !important;\n        color: #333333 !important;\n        font-family: \"Raleway\", Arial, sans-serif !important;\n        font-size: 16px !important;\n        line-height: 1.75 !important;\n        text-align: justify;\n    }\n\n    .dlw-prod-wrapper strong {\n        color: var(--dlw-blue-dark);\n        font-weight: 700;\n    }\n\n    \/* Lists *\/\n    .dlw-prod-wrapper .dlw-list {\n        display: flex;\n        flex-direction: column;\n        gap: 12px;\n        width: 100%;\n        margin: 8px 0 26px !important;\n        padding: 0 !important;\n    }\n\n    .dlw-prod-wrapper .dlw-list-item {\n        position: relative;\n        display: block;\n        width: 100%;\n        margin: 0 !important;\n        padding: 0 0 0 22px !important;\n        color: #333333;\n        font-size: 16px;\n        line-height: 1.65;\n    }\n\n    .dlw-prod-wrapper .dlw-list-item::before {\n        position: absolute;\n        top: 0;\n        left: 2px;\n        color: var(--dlw-blue-cyan);\n        font-size: 18px;\n        font-weight: 700;\n        content: \"\u2022\";\n    }\n\n    \/* Clinical applications *\/\n    .dlw-prod-wrapper .dlw-check-list {\n        display: flex;\n        flex-direction: column;\n        gap: 14px;\n        width: 100%;\n        margin: 8px 0 26px !important;\n        padding: 0 !important;\n    }\n\n    .dlw-prod-wrapper .dlw-check-item {\n        position: relative;\n        display: block;\n        width: 100%;\n        padding: 0 0 0 34px !important;\n        color: #333333;\n        font-size: 16px;\n        line-height: 1.65;\n    }\n\n    .dlw-prod-wrapper .dlw-check-item::before {\n        position: absolute;\n        top: 2px;\n        left: 0;\n        display: flex;\n        align-items: center;\n        justify-content: center;\n        width: 22px;\n        height: 22px;\n        border-radius: 50%;\n        background-color: #52ace5;\n        color: #ffffff;\n        font-family: Arial, sans-serif;\n        font-size: 14px;\n        font-weight: 700;\n        line-height: 1;\n        content: \"\u2713\";\n    }\n\n    \/* Technology *\/\n    .dlw-prod-wrapper .dlw-tech-box {\n        width: 100%;\n        margin: 10px 0 28px !important;\n        padding: 22px 24px !important;\n        border: 1px solid var(--dlw-border);\n        border-radius: 7px;\n        background-color: var(--dlw-light-bg);\n    }\n\n    .dlw-prod-wrapper .dlw-tech-line {\n        position: relative;\n        display: block;\n        margin: 0 0 11px !important;\n        padding: 0 0 0 20px !important;\n        color: #333333;\n        line-height: 1.65;\n    }\n\n    .dlw-prod-wrapper .dlw-tech-line:last-child {\n        margin-bottom: 0 !important;\n    }\n\n    .dlw-prod-wrapper .dlw-tech-line::before {\n        position: absolute;\n        top: 0;\n        left: 1px;\n        color: var(--dlw-blue-cyan);\n        font-weight: 700;\n        content: \"\u2022\";\n    }\n\n    \/* Key aspects *\/\n    .dlw-prod-wrapper .dlw-key-grid {\n        display: grid;\n        grid-template-columns: repeat(2, minmax(0, 1fr));\n        gap: 28px 36px;\n        width: 100%;\n        margin: 0 0 30px !important;\n        padding: 36px 40px !important;\n        border-radius: 10px;\n        background-color: #f4f8fc;\n        background-image:\n            radial-gradient(\n                circle,\n                rgba(0, 161, 224, 0.09) 1.5px,\n                transparent 1.5px\n            );\n        background-position: 0 0;\n        background-size: 38px 38px;\n    }\n\n    .dlw-prod-wrapper .dlw-key-item {\n        position: relative;\n        min-width: 0;\n        padding: 0 0 0 38px;\n    }\n\n    .dlw-prod-wrapper .dlw-key-icon {\n        position: absolute;\n        top: 1px;\n        left: 0;\n        display: flex;\n        align-items: center;\n        justify-content: center;\n        width: 24px;\n        height: 24px;\n        border-radius: 50%;\n        background-color: #52ace5;\n        color: #ffffff;\n        font-family: Arial, sans-serif;\n        font-size: 15px;\n        font-weight: 700;\n        line-height: 1;\n    }\n\n    .dlw-prod-wrapper .dlw-key-text {\n        display: block;\n        margin: 0 !important;\n        color: #4f5358;\n        font-family: \"Raleway\", Arial, sans-serif;\n        font-size: 15px;\n        line-height: 1.65;\n    }\n\n    \/* Presentation details dropdown *\/\n    .dlw-prod-wrapper .dlw-presentation-details {\n        width: 100%;\n        margin: 42px 0 0 !important;\n        border: 1px solid var(--dlw-border);\n        border-radius: 10px;\n        background-color: #ffffff;\n        overflow: hidden;\n        transition:\n            border-color 0.2s ease,\n            box-shadow 0.2s ease;\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-details[open] {\n        border-color: rgba(0, 161, 224, 0.45);\n        box-shadow: 0 10px 30px rgba(0, 58, 112, 0.08);\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-summary {\n        position: relative;\n        display: flex;\n        align-items: center;\n        width: 100%;\n        min-height: 72px;\n        margin: 0 !important;\n        padding: 19px 64px 19px 24px !important;\n        background-color: var(--dlw-light-bg);\n        color: var(--dlw-blue-dark);\n        font-family: \"Playfair Display\", Georgia, serif;\n        font-size: 24px;\n        font-weight: 600;\n        line-height: 1.3;\n        cursor: pointer;\n        list-style: none;\n        user-select: none;\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-summary::-webkit-details-marker {\n        display: none;\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-summary::before {\n        position: absolute;\n        bottom: 15px;\n        left: 24px;\n        width: 44px;\n        height: 3px;\n        background-color: var(--dlw-blue-cyan);\n        content: \"\";\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-summary::after {\n        position: absolute;\n        top: 50%;\n        right: 24px;\n        display: flex;\n        align-items: center;\n        justify-content: center;\n        width: 30px;\n        height: 30px;\n        border-radius: 50%;\n        background-color: var(--dlw-blue-cyan);\n        color: #ffffff;\n        font-family: Arial, sans-serif;\n        font-size: 22px;\n        font-weight: 400;\n        line-height: 1;\n        content: \"+\";\n        transform: translateY(-50%);\n        transition:\n            transform 0.2s ease,\n            background-color 0.2s ease;\n    }\n\n    .dlw-prod-wrapper\n        .dlw-presentation-details[open]\n        .dlw-presentation-summary::after {\n        background-color: var(--dlw-blue-dark);\n        content: \"\u2212\";\n        transform: translateY(-50%) rotate(180deg);\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-summary:hover {\n        background-color: #f2f8fc;\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-summary:focus-visible {\n        outline: 3px solid rgba(0, 161, 224, 0.35);\n        outline-offset: -3px;\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-content {\n        padding: 8px 26px 28px !important;\n        border-top: 1px solid var(--dlw-border);\n        background-color: #ffffff;\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-content > *:first-child {\n        margin-top: 24px !important;\n    }\n\n    .dlw-prod-wrapper .dlw-presentation-content > *:last-child {\n        margin-bottom: 0 !important;\n    }\n\n    @media (max-width: 768px) {\n        .dlw-prod-wrapper {\n            width: 100% !important;\n            max-width: 100% !important;\n        }\n\n        .dlw-prod-wrapper .dlw-intro {\n            padding: 18px !important;\n            text-align: left;\n        }\n\n        .dlw-prod-wrapper h2.dlw-section-title {\n            margin-top: 34px !important;\n            font-size: 25px !important;\n        }\n\n        .dlw-prod-wrapper h3.dlw-subsection-title {\n            font-size: 21px !important;\n        }\n\n        .dlw-prod-wrapper p.dlw-text {\n            text-align: left;\n        }\n\n        .dlw-prod-wrapper .dlw-tech-box {\n            padding: 18px !important;\n        }\n\n        .dlw-prod-wrapper .dlw-key-grid {\n            grid-template-columns: 1fr;\n            gap: 24px;\n            padding: 28px 22px !important;\n        }\n\n        .dlw-prod-wrapper .dlw-presentation-summary {\n            min-height: 68px;\n            padding: 17px 58px 17px 18px !important;\n            font-size: 21px;\n        }\n\n        .dlw-prod-wrapper .dlw-presentation-summary::before {\n            bottom: 13px;\n            left: 18px;\n            width: 40px;\n        }\n\n        .dlw-prod-wrapper .dlw-presentation-summary::after {\n            right: 17px;\n        }\n\n        .dlw-prod-wrapper .dlw-presentation-content {\n            padding: 6px 19px 24px !important;\n        }\n    }\n<\/style>\n\n<div class=\"dlw-prod-wrapper\">\n\n    <div class=\"dlw-intro\">\n        In vitro diagnostic kit based on real-time PCR for the qualitative detection of the ApoE*E2, ApoE*E3 and ApoE*E4 alleles in human genomic DNA.\n    <\/div>\n\n    <h2 class=\"dlw-section-title\">Detailed Description<\/h2>\n\n    <h3 class=\"dlw-subsection-title\">Operating principle<\/h3>\n\n    <p class=\"dlw-text\">\n        The kit is based on the specific amplification of target sequences by Real-Time PCR, using specific fluorescent probes.\n    <\/p>\n\n    <p class=\"dlw-text\">\n        Allelic discrimination is performed using labelled probes:\n    <\/p>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">\n            FAM (6-carboxyfluorescein) to detect cysteine (Cys)\n        <\/span>\n\n        <span class=\"dlw-list-item\">\n            HEX (hexachlorofluorescein) to detect arginine (Arg)\n        <\/span>\n    <\/div>\n\n    <p class=\"dlw-text\">\n        The fluorescent signal emitted during amplification makes it possible to identify the amino acid substitutions corresponding to the ApoE*E2, E3 and E4 alleles.\n    <\/p>\n\n    <h3 class=\"dlw-subsection-title\">Clinical applications<\/h3>\n\n    <div class=\"dlw-check-list\">\n        <span class=\"dlw-check-item\">\n            Genotyping of the ApoE gene in human samples.\n        <\/span>\n\n        <span class=\"dlw-check-item\">\n            Assessment of the genetic risk associated with Alzheimer\u2019s disease (AD).\n        <\/span>\n\n        <span class=\"dlw-check-item\">\n            Support for studies on lipid metabolism and neurodegenerative diseases.\n        <\/span>\n\n        <span class=\"dlw-check-item\">\n            Potential utility in predicting response to treatments (e.g. response to anticholinesterase drugs according to ApoE genotype).\n        <\/span>\n    <\/div>\n\n    <h3 class=\"dlw-subsection-title\">Results or key indicators<\/h3>\n\n    <p class=\"dlw-text\">\n        Qualitative identification of the alleles:\n    <\/p>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">ApoE*E2 (Cys112-Cys158)<\/span>\n        <span class=\"dlw-list-item\">ApoE*E3 (Cys112-Arg158)<\/span>\n        <span class=\"dlw-list-item\">ApoE*E4 (Arg112-Arg158)<\/span>\n    <\/div>\n\n    <p class=\"dlw-text\">\n        Genotype determination:\n    <\/p>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">\n            Homozygous: E2\/E2, E3\/E3, E4\/E4\n        <\/span>\n\n        <span class=\"dlw-list-item\">\n            Heterozygous: E2\/E3, E2\/E4, E3\/E4\n        <\/span>\n    <\/div>\n\n    <h3 class=\"dlw-subsection-title\">Technology used<\/h3>\n\n    <div class=\"dlw-tech-box\">\n        <span class=\"dlw-tech-line\">\n            Real-time PCR (Real-Time PCR)\n        <\/span>\n\n        <span class=\"dlw-tech-line\">\n            Specific fluorogenic probes (TaqMan\u00ae-type technology)\n        <\/span>\n\n        <span class=\"dlw-tech-line\">\n            Multichannel detection (FAM \/ HEX)\n        <\/span>\n\n        <span class=\"dlw-tech-line\">\n            Compatible with multiple platforms:\n        <\/span>\n\n        <div class=\"dlw-list\">\n            <span class=\"dlw-list-item\">\n                Rotor-Gene\u00ae Q (Qiagen)\n            <\/span>\n\n            <span class=\"dlw-list-item\">\n                CFX96 \/ Opus (Bio-Rad)\n            <\/span>\n\n            <span class=\"dlw-list-item\">\n                Applied Biosystems\u00ae 7500\n            <\/span>\n\n            <span class=\"dlw-list-item\">\n                ELITe InGenius\u00ae and ELITe BeGenius\u00ae\n            <\/span>\n        <\/div>\n    <\/div>\n\n    <h2 class=\"dlw-section-title\">Key Aspects<\/h2>\n\n    <div class=\"dlw-key-grid\">\n\n        <div class=\"dlw-key-item\">\n            <span class=\"dlw-key-icon\" aria-hidden=\"true\">\u2713<\/span>\n            <span class=\"dlw-key-text\">\n                Specific detection of the ApoE E2, E3 and E4 alleles.\n            <\/span>\n        <\/div>\n\n        <div class=\"dlw-key-item\">\n            <span class=\"dlw-key-icon\" aria-hidden=\"true\">\u2713<\/span>\n            <span class=\"dlw-key-text\">\n                Technology based on Real-Time PCR with fluorescent probes.\n            <\/span>\n        <\/div>\n\n        <div class=\"dlw-key-item\">\n            <span class=\"dlw-key-icon\" aria-hidden=\"true\">\u2713<\/span>\n            <span class=\"dlw-key-text\">\n                High specificity through allelic discrimination (FAM\/HEX).\n            <\/span>\n        <\/div>\n\n        <div class=\"dlw-key-item\">\n            <span class=\"dlw-key-icon\" aria-hidden=\"true\">\u2713<\/span>\n            <span class=\"dlw-key-text\">\n                Compatible with multiple real-time PCR platforms.\n            <\/span>\n        <\/div>\n\n        <div class=\"dlw-key-item\">\n            <span class=\"dlw-key-icon\" aria-hidden=\"true\">\u2713<\/span>\n            <span class=\"dlw-key-text\">\n                Includes positive controls (wild type and mutant) and a negative control.\n            <\/span>\n        <\/div>\n\n        <div class=\"dlw-key-item\">\n            <span class=\"dlw-key-icon\" aria-hidden=\"true\">\u2713<\/span>\n            <span class=\"dlw-key-text\">\n                Applicable to Alzheimer\u2019s disease risk studies.\n            <\/span>\n        <\/div>\n\n        <div class=\"dlw-key-item\">\n            <span class=\"dlw-key-icon\" aria-hidden=\"true\">\u2713<\/span>\n            <span class=\"dlw-key-text\">\n                Ready-to-use format.\n            <\/span>\n        <\/div>\n\n    <\/div>\n\n    <details class=\"dlw-presentation-details\">\n        <summary class=\"dlw-presentation-summary\">\n            Presentation Details\n        <\/summary>\n\n        <div class=\"dlw-presentation-content\">\n\n            <p class=\"dlw-text\">\n                <strong>Kit format:<\/strong> 32 tests (ref. EER018032)\n            <\/p>\n\n            <h4 class=\"dlw-detail-title\">Contents:<\/h4>\n\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">\n                    Amplification mixes (aa112 and aa158)\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Oligonucleotide mixes (aa112 and aa158)\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Control 1 (Cys, wild type)\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Control 2 (Arg, mutant)\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Reaction blank (BM)\n                <\/span>\n            <\/div>\n\n            <h4 class=\"dlw-detail-title\">Configuration:<\/h4>\n\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">\n                    Two separate boxes (aa112 and aa158)\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Reaction volume: 25 \u00b5l\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Required DNA: 100\u2013250 ng per reaction\n                <\/span>\n            <\/div>\n\n            <h4 class=\"dlw-detail-title\">Capacity:<\/h4>\n\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">\n                    Up to 32 determinations\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Validated for use in multiple sessions\n                <\/span>\n            <\/div>\n\n            <h4 class=\"dlw-detail-title\">Storage conditions:<\/h4>\n\n            <div class=\"dlw-list\">\n                <span class=\"dlw-list-item\">\n                    -22\u00b0C to -18\u00b0C\n                <\/span>\n\n                <span class=\"dlw-list-item\">\n                    Maximum of 6 freeze\/thaw cycles\n                <\/span>\n            <\/div>\n\n            <p class=\"dlw-text\">\n                <strong>Sample type:<\/strong> Peripheral blood in EDTA\n            <\/p>\n\n        <\/div>\n    <\/details>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>In vitro diagnostic kit based on real-time PCR for the qualitative detection of the ApoE*E2, ApoE*E3 and ApoE*E4 alleles in human genomic DNA. Detailed Description Operating principle The kit is based on the specific amplification of target sequences by Real-Time PCR, using specific fluorescent probes. Allelic discrimination is performed using labelled probes: FAM (6-carboxyfluorescein) to [&hellip;]<\/p>\n","protected":false},"featured_media":28368,"template":"","tecnologias":[275],"marcas":[234],"familias":[283,425],"class_list":["post-28370","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-rt-pcr-en","marcas-clonit-en","familias-molecular-genetics","familias-hereditary_and_or_rare_diseases"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28370","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28370\/revisions"}],"predecessor-version":[{"id":28371,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28370\/revisions\/28371"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/28368"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=28370"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=28370"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=28370"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=28370"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}