{"id":28530,"date":"2026-10-06T12:44:53","date_gmt":"2026-10-06T12:44:53","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=28530"},"modified":"2026-10-06T12:47:08","modified_gmt":"2026-10-06T12:47:08","slug":"genvinset-fiifv-multiplex","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/genvinset-fiifv-multiplex\/","title":{"rendered":"Genvinset\u00ae FIIFV multiplex"},"content":{"rendered":"\n<!-- Complete HTML ready for WordPress \/ Bricks -->\n\n<style>\n@import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:wght@500;600&family=Raleway:wght@400;500;600;700&display=swap');\n\n#dlw-fiifv-en,\n#dlw-fiifv-en *,\n#dlw-fiifv-en *::before,\n#dlw-fiifv-en *::after {\n    box-sizing: border-box !important;\n}\n\n#dlw-fiifv-en {\n    --dlw-blue-dark: #003a70;\n    --dlw-blue-cyan: #00a1e0;\n    --dlw-light-bg: #f9fbfd;\n    --dlw-grey-bg: #f4f6f8;\n    --dlw-border: #e1e8f0;\n    --dlw-text: 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font-size: 20px !important;\n    }\n\n    #dlw-fiifv-en .dlw-details-content {\n        padding: 23px 19px 25px !important;\n    }\n}\n<\/style>\n\n<div id=\"dlw-fiifv-en\" class=\"dlw-prod-wrapper\">\n\n    <div class=\"dlw-intro\">\n        <p><em>In vitro<\/em> diagnostic kit for the simultaneous detection of the G20210A mutation in the prothrombin gene (FII) and the G1691A mutation in Factor V using real-time PCR.<\/p>\n    <\/div>\n\n    <h2>Detailed description<\/h2>\n\n    <h3>Operating principle<\/h3>\n\n    <p>The assay is based on the real-time PCR (Real-Time PCR) amplification of specific regions of the FII and FV factor genes containing the mutations of interest. It uses TaqMan\u00ae hydrolysis probes labelled with fluorophores (FAM, HEX, ROX and Cy5) that enable allelic discrimination. The fluorescent signal generated during amplification is proportional to the amplified DNA and enables genotype determination.<\/p>\n\n    <h3>Clinical applications<\/h3>\n\n    <p>Qualitative detection of the G20210A (FII) and G1691A (Factor V Leiden) mutations, associated with the risk of venous thrombosis and inherited thrombophilia.<\/p>\n\n    <h2>Key aspects and benefits<\/h2>\n\n    <div class=\"dlw-key-panel\">\n        <div class=\"dlw-key-grid\">\n            <div class=\"dlw-key-item\">Multiplex detection: both mutations are detected simultaneously in a single reaction (G20210A and G1691A).<\/div>\n            <div class=\"dlw-key-item\">High specificity<\/div>\n            <div class=\"dlw-key-item\">Clear fluorescence-based interpretation<\/div>\n            <div class=\"dlw-key-item\">Compatible with multiple real-time PCR instruments<\/div>\n        <\/div>\n    <\/div>\n\n    <h3>Key indicators<\/h3>\n\n    <div class=\"dlw-indicators\">\n        <div class=\"dlw-indicator\">\n            <span class=\"dlw-indicator-value\">100%<\/span>\n            <span class=\"dlw-indicator-label\">Concordance<\/span>\n        <\/div>\n\n        <div class=\"dlw-indicator\">\n            <span class=\"dlw-indicator-value\">100%<\/span>\n            <span class=\"dlw-indicator-label\">Repeatability and reproducibility<\/span>\n        <\/div>\n\n        <div class=\"dlw-indicator\">\n            <span class=\"dlw-indicator-value\">High<\/span>\n            <span class=\"dlw-indicator-label\">Analytical specificity<\/span>\n        <\/div>\n    <\/div>\n\n    <h3>Intended users<\/h3>\n\n    <p>Technical personnel trained and qualified in molecular biology and genetic analysis in specialised clinical laboratories.<\/p>\n\n    <h3>Considerations or limitations<\/h3>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Detects only the specified mutations<\/span>\n        <span class=\"dlw-list-item\">Variants in primer- or probe-binding regions may affect the interpretation of results.<\/span>\n        <span class=\"dlw-list-item\">The results must be used together with other clinical and laboratory data.<\/span>\n    <\/div>\n\n    <details class=\"dlw-details\">\n        <summary>Presentation details<\/summary>\n\n        <div class=\"dlw-details-content\">\n            <span class=\"dlw-details-heading\">Available references<\/span>\n\n            <div class=\"dlw-reference-grid\">\n                <div class=\"dlw-reference-card\">\n                    <span class=\"dlw-reference-code\">GVS-FIIFV-24<\/span>\n                    <span class=\"dlw-reference-tests\">24 tests.<\/span>\n                <\/div>\n\n                <div class=\"dlw-reference-card\">\n                    <span class=\"dlw-reference-code\">GVS-FIIFV-48<\/span>\n                    <span class=\"dlw-reference-tests\">48 tests.<\/span>\n                <\/div>\n\n                <div class=\"dlw-reference-card\">\n                    <span class=\"dlw-reference-code\">GVS-FIIFV-96<\/span>\n                    <span class=\"dlw-reference-tests\">96 tests (on request).<\/span>\n                <\/div>\n            <\/div>\n\n            <div class=\"dlw-detail-row\">\n                <strong>Kit contents:<\/strong> each kit contains Master Mix, the primer mixture (Primer Mix), controls (WT and MUT), and a reaction blank (Reaction Blank).\n            <\/div>\n\n            <div class=\"dlw-detail-row\">\n                <strong>Storage conditions:<\/strong> Store between -30\u00b0C and -18\u00b0C.\n            <\/div>\n        <\/div>\n    <\/details>\n\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>In vitro diagnostic kit for the simultaneous detection of the G20210A mutation in the prothrombin gene (FII) and the G1691A mutation in Factor V using real-time PCR. Detailed description Operating principle The assay is based on the real-time PCR (Real-Time PCR) amplification of specific regions of the FII and FV factor genes containing the mutations [&hellip;]<\/p>\n","protected":false},"featured_media":28528,"template":"","tecnologias":[275],"marcas":[225],"familias":[307],"class_list":["post-28530","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-rt-pcr-en","marcas-blackhills-diagnostic-resources-en","familias-hematology-and-blood-bank"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28530","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28530\/revisions"}],"predecessor-version":[{"id":28531,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28530\/revisions\/28531"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/28528"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=28530"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=28530"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=28530"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=28530"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}