{"id":28552,"date":"2026-10-06T14:03:48","date_gmt":"2026-10-06T14:03:48","guid":{"rendered":"https:\/\/www.dlongwood.com\/?post_type=productos&#038;p=28552"},"modified":"2026-10-06T14:09:15","modified_gmt":"2026-10-06T14:09:15","slug":"nimotest-fetal-rhd-qpcr-kit","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/nimotest-fetal-rhd-qpcr-kit\/","title":{"rendered":"NimoTest\u00ae Fetal RHD qPCR kit"},"content":{"rendered":"\n<!-- Complete HTML ready for WordPress \/ Bricks -->\n\n<style>\n@import url('https:\/\/fonts.googleapis.com\/css2?family=Playfair+Display:wght@500;600&family=Raleway:wght@400;500;600;700&display=swap');\n\n#dlw-nimotest-en,\n#dlw-nimotest-en *,\n#dlw-nimotest-en *::before,\n#dlw-nimotest-en *::after,\n#dlw-nimotest-modal-en,\n#dlw-nimotest-modal-en * {\n    box-sizing: border-box !important;\n}\n\n#dlw-nimotest-en {\n    --dlw-blue-dark: #003a70;\n    --dlw-blue-cyan: #00a1e0;\n    --dlw-light-bg: #f9fbfd;\n    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padding: 18px !important;\n    }\n\n    #dlw-nimotest-modal-en .dlw-modal-img {\n        max-width: 96vw !important;\n        max-height: 86vh !important;\n    }\n}\n<\/style>\n\n<div id=\"dlw-nimotest-en\" class=\"dlw-prod-wrapper\">\n\n    <figure class=\"dlw-figure dlw-figure-first\">\n        <img decoding=\"async\"\n            src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/10\/1.webp\"\n            alt=\"NIMoTest Fetal RHD qPCR kit\"\n            class=\"dlw-prod-img\"\n            onclick=\"openDlwNimotestModalEn(this.src, this.alt)\"\n        >\n    <\/figure>\n\n    <div class=\"dlw-intro\">\n        <p>NIMoTest\u00ae Fetal RHD qPCR kit is a kit for determining the fetal RHD genotype from maternal plasma. It is intended for the monitoring of RhD-negative pregnant women to guide anti-RhD immunoprophylaxis and adapt the monitoring of immunised women.<\/p>\n    <\/div>\n\n    <h2>Detailed description<\/h2>\n\n    <p>The kit is designed for duplex quantitative PCR (pPCR) amplification of exons 5 and 7 of the RHD gene in circulating cell-free fetal DNA (cffDNA) samples obtained from the maternal plasma of non-immunised RhD-negative pregnant women. Results are interpreted by analysing the Ct curves using NIMoSoft\u00ae software.<\/p>\n\n    <h3>Operating principle<\/h3>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">The kit is designed to amplify specific regions of exons 5 and 7 of the RHD gene by pPCR, with a duration of approximately 1 hour from the extraction of the cffDNA (cell-free fetal DNA) present in maternal plasma.<\/span>\n\n        <span class=\"dlw-list-item\">It includes a small extraction control (EC) that must be added to the plasma before lysis to validate the extraction process and verify the absence of PCR inhibitors.<\/span>\n\n        <span class=\"dlw-list-item\">Results are interpreted using NIMoSoft\u00ae analysis software.<\/span>\n    <\/div>\n\n    <figure class=\"dlw-figure\">\n        <img decoding=\"async\"\n            src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/10\/2.webp\"\n            alt=\"NIMoTest Fetal RHD qPCR kit workflow\"\n            class=\"dlw-prod-img\"\n            onclick=\"openDlwNimotestModalEn(this.src, this.alt)\"\n        >\n    <\/figure>\n\n    <h3>Clinical applications<\/h3>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">It is intended for determining the fetal RHD genotype from the maternal plasma of non-immunised RhD-negative pregnant women as part of non-invasive biological prenatal diagnosis.<\/span>\n\n        <span class=\"dlw-list-item\">It supports decisions regarding the administration of anti-D prophylaxis only in pregnancies with an RhD-positive fetus to control the risk of fetal and neonatal haemolytic disease associated with anti-RhD.<\/span>\n\n        <span class=\"dlw-list-item\">Indicated for use from week 11 of pregnancy.<\/span>\n    <\/div>\n\n    <h2>Key aspects and benefits<\/h2>\n\n    <div class=\"dlw-key-panel\">\n        <div class=\"dlw-key-grid\">\n            <div class=\"dlw-key-item\">Multiplex detection of exons 5 and 7 of the RhD gene with ready-to-use reagents.<\/div>\n\n            <div class=\"dlw-key-item\">Includes an extraction control (small DNA fragment) to validate extraction and the absence of inhibitors.<\/div>\n\n            <div class=\"dlw-key-item dlw-key-wide\">Provides a simplified workflow.<\/div>\n\n            <div class=\"dlw-key-image\">\n                <img decoding=\"async\"\n                    src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/10\/3-en.webp\"\n                    alt=\"Simplified NIMoTest workflow\"\n                    class=\"dlw-prod-img\"\n                    onclick=\"openDlwNimotestModalEn(this.src, this.alt)\"\n                >\n            <\/div>\n\n            <div class=\"dlw-key-item\">Includes NIMoSoft analysis software for complete traceability, validation, interpretation and LIMS integration.<\/div>\n\n            <div class=\"dlw-key-item\">Enables results to be obtained in approximately 1 hour from DNA extraction.<\/div>\n        <\/div>\n    <\/div>\n\n    <h3>Technology used<\/h3>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">Validated with QuantStudio 7, LightCycler 480 II, M2000rt and qTower3 instruments.<\/span>\n\n        <span class=\"dlw-list-item\">Validated with extraction methods including MagNAPure 96, MagNA Pure 24, MagPurix, LabTurbo-24 and Chemagic 360d.<\/span>\n    <\/div>\n\n    <h3>Intended users<\/h3>\n\n    <p>Immunohaematology laboratories, blood banks and transfusion services interested in fetal RhD genotyping in non-immunised RhD-negative pregnant women.<\/p>\n\n    <h3>Additional considerations<\/h3>\n\n    <div class=\"dlw-list\">\n        <span class=\"dlw-list-item\">DNA extraction is critical due to the low concentration of cffDNA in maternal plasma.<\/span>\n\n        <span class=\"dlw-list-item\">The test is only suitable from week 11 of pregnancy.<\/span>\n\n        <span class=\"dlw-list-item\">Heparin may inhibit PCR; the validated starting material is plasma obtained from EDTA blood.<\/span>\n    <\/div>\n\n    <details class=\"dlw-details\">\n        <summary>Presentation details<\/summary>\n\n        <div class=\"dlw-details-content\">\n            <div class=\"dlw-detail-row\">\n                <strong>Kit size:<\/strong> 21 samples per 96-reaction kit to allow testing in triplicate and the extraction control.\n            <\/div>\n\n            <div class=\"dlw-detail-row\">\n                <strong>Reference number:<\/strong> NIMRHDQ96.\n            <\/div>\n\n            <div class=\"dlw-detail-row\">\n                Includes a positive control, negative control and extraction control.\n            <\/div>\n        <\/div>\n    <\/details>\n\n<\/div>\n\n<div\n    id=\"dlw-nimotest-modal-en\"\n    role=\"dialog\"\n    aria-modal=\"true\"\n    aria-hidden=\"true\"\n    aria-label=\"Enlarged image view\"\n>\n    <button\n        type=\"button\"\n        class=\"dlw-modal-close\"\n        aria-label=\"Close image\"\n        onclick=\"closeDlwNimotestModalEn()\"\n    >\u00d7<\/button>\n\n    <img decoding=\"async\"\n        src=\"\"\n        alt=\"\"\n        class=\"dlw-modal-img\"\n        id=\"dlw-nimotest-modal-image-en\"\n        onclick=\"closeDlwNimotestModalEn()\"\n    >\n<\/div>\n\n<script>\n(function () {\n    var modal = document.getElementById(\"dlw-nimotest-modal-en\");\n    var modalImage = document.getElementById(\"dlw-nimotest-modal-image-en\");\n    var previousBodyOverflow = \"\";\n\n    if (!modal || !modalImage) {\n        return;\n    }\n\n    if (modal.parentNode !== document.body) {\n        document.body.appendChild(modal);\n    }\n\n    window.openDlwNimotestModalEn = function (source, alternativeText) {\n        previousBodyOverflow = document.body.style.overflow;\n        modalImage.src = source;\n        modalImage.alt = alternativeText || \"\";\n        modal.classList.add(\"dlw-modal-open\");\n        modal.setAttribute(\"aria-hidden\", \"false\");\n        document.body.style.overflow = \"hidden\";\n    };\n\n    window.closeDlwNimotestModalEn = function () {\n        modal.classList.remove(\"dlw-modal-open\");\n        modal.setAttribute(\"aria-hidden\", \"true\");\n        document.body.style.overflow = previousBodyOverflow;\n    };\n\n    modal.addEventListener(\"click\", function (event) {\n        if (event.target === modal) {\n            window.closeDlwNimotestModalEn();\n        }\n    });\n\n    document.addEventListener(\"keydown\", function (event) {\n        if (\n            event.key === \"Escape\" &&\n            modal.classList.contains(\"dlw-modal-open\")\n        ) {\n            window.closeDlwNimotestModalEn();\n        }\n    });\n})();\n<\/script>\n","protected":false},"excerpt":{"rendered":"<p>NIMoTest\u00ae Fetal RHD qPCR kit is a kit for determining the fetal RHD genotype from maternal plasma. It is intended for the monitoring of RhD-negative pregnant women to guide anti-RhD immunoprophylaxis and adapt the monitoring of immunised women. Detailed description The kit is designed for duplex quantitative PCR (pPCR) amplification of exons 5 and 7 [&hellip;]<\/p>\n","protected":false},"featured_media":28549,"template":"","tecnologias":[275],"marcas":[540],"familias":[307],"class_list":["post-28552","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-rt-pcr-en","marcas-bedia-en","familias-hematology-and-blood-bank"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28552","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28552\/revisions"}],"predecessor-version":[{"id":28553,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/28552\/revisions\/28553"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/28549"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=28552"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=28552"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=28552"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=28552"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}