{"id":8244,"date":"2019-12-02T13:35:24","date_gmt":"2019-12-02T13:35:24","guid":{"rendered":"https:\/\/www.dlongwood.com\/fusionplex-lung-v2-3\/"},"modified":"2025-05-15T10:07:26","modified_gmt":"2025-05-15T10:07:26","slug":"fusionplex-lung-v2","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/fusionplex-lung-v2\/","title":{"rendered":"FusionPlex\u00ae Lung v2"},"content":{"rendered":"\n

The FusionPlex\u00ae Lung v2 panel is a targeted Next-Generation Sequencing (NGS) <\/strong>research product that expands on the well-adopted FusionPlex\u00ae Lung panel to further drive discovery. The updated design covers newly published fusion breakpoints <\/strong>and isoforms of interest in key gene targets <\/strong>(including the NTRK<\/em>s) and aligns with existing guidelines for non-small cell lung cancer<\/strong> research, including NCCN and ESMO (ESCAT).<\/p>\n\n\n\n

FusionPlex\u00ae uses RNA<\/strong> instead of DNA as input material, which allows for more accurate and more cost-effective fusion detection<\/strong> than DNA-based hybrid capture techniques. FusionPlex\u00ae places gene-specific primers near known fusion breakpoints to identify RNA abnormalities with a single primer. Because FusionPlex\u00ae can identify known and novel alterations<\/strong> with a limited number of primers, it can detect fusions more efficiently and accurately than DNA-based approaches.<\/p>\n\n\n\n

All gene content from the Lung v1 panel <\/strong>is covered in v2, including EGFR vIII<\/em> and MET<\/em> exon 14 skipping events along with prominent ALK, BRAF, FGFR, NRG1, NTRK, RET<\/em>, and ROS1<\/em> fusions and select point mutations.<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"<\/a><\/figure><\/div>\n\n\n\n
<\/div>\n\n\n\n

The panel is powered by Anchored Multiplex PCR (AMP\u2122) <\/strong>target enrichment chemistry to detect fusions in a single sequencing product, even without prior knowledge of fusion partners or breakpoints.<\/p>\n\n\n\n

Archer\nAnalysis software allows the analysis and interpretation of the data.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Comprehensive Fusion Detection<\/strong><\/p>\n\n\n\n

AMP\u2122 chemistry utilizes open-ended targeted amplification to identify gene fusions whether or not the fusion partner is known.<\/p>\n\n\n\n

Splice Variant<\/strong><\/p>\n\n\n\n

Detect cancer-associated isoforms and alternative splicing events.<\/p>\n\n\n\n

Low Input<\/strong><\/p>\n\n\n\n

Purpose-built for wide sample type compatibility, including low-input and degraded samples such as FFPE tissue.<\/p>\n\n\n\n

Simple Workflow<\/strong>, Lyophilized Reagents <\/strong><\/p>\n\n\n\n

Easy-to-handle lyophilized reagents and a simple workflow to minimize errors and reduce turn-around time.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Gene Targets<\/strong><\/td>17<\/td><\/tr>
Input Nucleic Acid Required*<\/strong><\/td>\u226510 ng<\/td><\/tr>
Unique Molecular On-Target %<\/strong><\/td>>96%<\/td><\/tr>
Sample Types<\/strong><\/td>Fresh, frozen and FFPE<\/td><\/tr>
Hands-On Time<\/strong><\/td>3.5 hours<\/td><\/tr>
Total Time<\/strong><\/td>1.5 days<\/td><\/tr>
Platform<\/strong><\/td>Illumina\u00ae e Ion Torrent\u2122<\/td><\/tr><\/tbody><\/table>
*Input mass requirements vary depending on type and quality. Unless the tumor cellularity and sample quality are high, 50ng of FFPE-derived nucleic acid should be considered the minimum recommendation. If input is not limiting, 200ng is recommended.<\/figcaption><\/figure>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

NGS assay for the study of 17 genes involved in lung cancer.<\/p>\n","protected":false},"featured_media":7591,"template":"","tecnologias":[36],"marcas":[217],"familias":[460,469,347,464],"class_list":["post-8244","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-archer-dx-en","familias-fusions","familias-respiratory-system","familias-solid-tumor","familias-tumor-types"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/8244","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":0,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/8244\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/7591"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=8244"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=8244"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=8244"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=8244"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}