{"id":8254,"date":"2019-12-02T12:43:02","date_gmt":"2019-12-02T12:43:02","guid":{"rendered":"https:\/\/www.dlongwood.com\/fusionplex-sarcoma-v2-3\/"},"modified":"2026-08-24T11:24:49","modified_gmt":"2026-08-24T11:24:49","slug":"fusionplex-sarcoma-v2","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/fusionplex-sarcoma-v2\/","title":{"rendered":"FusionPlex\u00ae Sarcoma v2"},"content":{"rendered":"\n<style>\n  .dlw-prod-wrapper {\n    width: 100% !important;\n    max-width: 720px !important;\n    margin: 0 auto !important;\n    padding: 28px 24px !important;\n    box-sizing: border-box !important;\n    color: #25313c;\n    font-family: \"Raleway\", Arial, sans-serif;\n    font-size: 16px;\n    line-height: 1.7;\n  }\n\n  .dlw-prod-wrapper *,\n  .dlw-prod-wrapper *::before,\n  .dlw-prod-wrapper *::after {\n    box-sizing: border-box;\n  }\n\n  .dlw-prod-wrapper .dlw-section {\n    margin: 0 0 46px;\n  }\n\n  .dlw-prod-wrapper .dlw-section:last-child {\n    margin-bottom: 0;\n  }\n\n  .dlw-prod-wrapper .dlw-title {\n    margin: 0 0 24px;\n    padding-bottom: 12px;\n    border-bottom: 3px solid #7bbf45;\n    color: #17385e;\n    font-family: \"Playfair Display\", Georgia, serif;\n    font-size: 30px;\n    font-weight: 700;\n    line-height: 1.2;\n    letter-spacing: 0.01em;\n  }\n\n  .dlw-prod-wrapper .dlw-subtitle {\n    margin: 0 0 12px;\n    color: #17385e;\n    font-family: \"Playfair Display\", Georgia, serif;\n    font-size: 22px;\n    font-weight: 700;\n    line-height: 1.3;\n  }\n\n  .dlw-prod-wrapper .dlw-card-title {\n    margin: 0 0 10px;\n    color: #17385e;\n    font-size: 18px;\n    font-weight: 700;\n    line-height: 1.35;\n  }\n\n  .dlw-prod-wrapper p {\n    margin: 0 0 18px;\n  }\n\n  .dlw-prod-wrapper p:last-child {\n    margin-bottom: 0;\n  }\n\n  .dlw-prod-wrapper .dlw-image-wrap {\n    margin: 28px auto;\n    text-align: center;\n  }\n\n  .dlw-prod-wrapper .dlw-image {\n    display: inline-block;\n    width: auto;\n    max-width: 90%;\n    height: auto;\n    margin: 0 auto;\n    border-radius: 10px;\n    box-shadow: 0 8px 24px rgba(23, 56, 94, 0.13);\n    cursor: zoom-in;\n    transition: transform 0.25s ease, box-shadow 0.25s ease;\n  }\n\n  .dlw-prod-wrapper .dlw-image:hover {\n    transform: translateY(-2px);\n    box-shadow: 0 12px 30px rgba(23, 56, 94, 0.2);\n  }\n\n  .dlw-prod-wrapper .dlw-card-grid {\n    display: grid;\n    grid-template-columns: repeat(2, minmax(0, 1fr));\n    gap: 18px;\n    margin-top: 24px;\n  }\n\n  .dlw-prod-wrapper .dlw-card {\n    padding: 22px;\n    border: 1px solid #dfe8ef;\n    border-top: 4px solid #7bbf45;\n    border-radius: 10px;\n    background: #f8fbfd;\n    box-shadow: 0 5px 16px rgba(23, 56, 94, 0.07);\n  }\n\n  .dlw-prod-wrapper .dlw-card p {\n    margin-bottom: 0;\n  }\n\n  .dlw-prod-wrapper .dlw-highlight {\n    margin: 24px 0;\n    padding: 20px 22px;\n    border-left: 5px solid #7bbf45;\n    border-radius: 0 10px 10px 0;\n    background: #eef7e8;\n    color: #17385e;\n  }\n\n  .dlw-prod-wrapper .dlw-highlight strong {\n    color: #17385e;\n  }\n\n  .dlw-prod-wrapper .dlw-list {\n    margin: 20px 0 0;\n    padding: 0;\n    list-style: none;\n  }\n\n  .dlw-prod-wrapper .dlw-list-item {\n    position: relative;\n    margin: 0 0 14px;\n    padding-left: 28px;\n  }\n\n  .dlw-prod-wrapper .dlw-list-item:last-child {\n    margin-bottom: 0;\n  }\n\n  .dlw-prod-wrapper .dlw-list-item::before {\n    position: absolute;\n    top: 0.7em;\n    left: 4px;\n    width: 9px;\n    height: 9px;\n    border-radius: 50%;\n    background: #7bbf45;\n    content: \"\";\n    transform: translateY(-50%);\n  }\n\n  .dlw-prod-wrapper .dlw-feature-grid {\n    display: grid;\n    grid-template-columns: repeat(2, minmax(0, 1fr));\n    gap: 16px;\n    margin-top: 22px;\n  }\n\n  .dlw-prod-wrapper .dlw-feature {\n    padding: 18px;\n    border-radius: 9px;\n    background: #f4f8fb;\n    border: 1px solid #dce6ed;\n  }\n\n  .dlw-prod-wrapper .dlw-feature:last-child:nth-child(odd) {\n    grid-column: 1 \/ -1;\n  }\n\n  .dlw-prod-wrapper .dlw-feature strong {\n    display: block;\n    margin-bottom: 5px;\n    color: #17385e;\n  }\n\n  .dlw-modal {\n    position: fixed;\n    z-index: 999999;\n    inset: 0;\n    display: none;\n    align-items: center;\n    justify-content: center;\n    padding: 24px;\n    background: rgba(8, 20, 34, 0.9);\n  }\n\n  .dlw-modal.dlw-modal-open {\n    display: flex;\n  }\n\n  .dlw-modal .dlw-modal-image {\n    display: block;\n    max-width: 94vw;\n    max-height: 88vh;\n    width: auto;\n    height: auto;\n    border-radius: 8px;\n    box-shadow: 0 16px 50px rgba(0, 0, 0, 0.45);\n  }\n\n  .dlw-modal .dlw-modal-close {\n    position: absolute;\n    top: 18px;\n    right: 22px;\n    width: 44px;\n    height: 44px;\n    padding: 0;\n    border: 0;\n    border-radius: 50%;\n    background: #ffffff;\n    color: #17385e;\n    font-family: Arial, sans-serif;\n    font-size: 30px;\n    line-height: 44px;\n    text-align: center;\n    cursor: pointer;\n  }\n\n  @media (max-width: 768px) {\n    .dlw-prod-wrapper {\n      padding: 20px 16px !important;\n      font-size: 15px;\n    }\n\n    .dlw-prod-wrapper .dlw-section {\n      margin-bottom: 36px;\n    }\n\n    .dlw-prod-wrapper .dlw-title {\n      font-size: 25px;\n    }\n\n    .dlw-prod-wrapper .dlw-subtitle {\n      font-size: 20px;\n    }\n\n    .dlw-prod-wrapper .dlw-image {\n      max-width: 100%;\n    }\n\n    .dlw-prod-wrapper .dlw-card-grid,\n    .dlw-prod-wrapper .dlw-feature-grid {\n      grid-template-columns: 1fr;\n    }\n\n    .dlw-prod-wrapper .dlw-feature:last-child:nth-child(odd) {\n      grid-column: auto;\n    }\n\n    .dlw-prod-wrapper .dlw-card,\n    .dlw-prod-wrapper .dlw-highlight {\n      padding: 18px;\n    }\n\n    .dlw-modal {\n      padding: 14px;\n    }\n\n    .dlw-modal .dlw-modal-close {\n      top: 10px;\n      right: 10px;\n    }\n  }\n<\/style>\n\n<div class=\"dlw-prod-wrapper\">\n  <section class=\"dlw-section\">\n    <h2 class=\"dlw-title\">OVERVIEW<\/h2>\n\n    <p>\n      The FUSIONPlex\u00ae Sarcoma v2 panel is designed for RNA-based\n      next-generation sequencing (NGS), using the patented Anchored Multiplex\n      PCR (AMP&#x2122;) technology.\n    <\/p>\n\n    <p>\n      AMP&#x2122; (Anchored Multiplex PCR) is a patented targeted enrichment platform\n      for next-generation sequencing (NGS). It is based on the use of\n      unidirectional gene-specific primers (GSPs) and molecular adapters with\n      unique molecular barcodes (MBCs), which are ligated to the genetic\n      material before amplification. This strategy enables efficient capture\n      and amplification of DNA, RNA or cfDNA fragments, even in the presence\n      of unknown mutations or highly fragmented sequences.\n    <\/p>\n\n    <div class=\"dlw-image-wrap\">\n      <img decoding=\"async\"\n        class=\"dlw-image\"\n        src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/08\/Imagen1-3.webp\"\n        alt=\"AMP Anchored Multiplex PCR technology\"\n        loading=\"lazy\"\n        onclick=\"openDlwModal(this.src, this.alt)\"\n      >\n    <\/div>\n\n    <p>\n      The anchored primer architecture supports a flexible design and reduces\n      information loss during library preparation.\n    <\/p>\n\n    <p>\n      Combined with Archer&#x2122; Analysis software, AMP&#x2122; enables systematic error\n      correction, read deduplication and accurate quantification of the\n      original molecules, facilitating the detection of single-nucleotide\n      variants, indels, gene fusions and copy-number alterations with high\n      sensitivity and specificity.\n    <\/p>\n  <\/section>\n\n  <section class=\"dlw-section\">\n    <h2 class=\"dlw-title\">RECOMMENDED TUMOUR TYPES<\/h2>\n\n    <div class=\"dlw-highlight\">\n      <strong>Sarcomas.<\/strong>\n    <\/div>\n\n    <div class=\"dlw-image-wrap\">\n      <img decoding=\"async\"\n        class=\"dlw-image\"\n        src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/08\/Imagen2-3.webp\"\n        alt=\"Tumour types recommended for the FUSIONPlex Sarcoma v2 panel\"\n        loading=\"lazy\"\n        onclick=\"openDlwModal(this.src, this.alt)\"\n      >\n    <\/div>\n  <\/section>\n\n  <section class=\"dlw-section\">\n    <h2 class=\"dlw-title\">PROTOCOL<\/h2>\n\n    <div class=\"dlw-card-grid\">\n      <article class=\"dlw-card\">\n        <h3 class=\"dlw-card-title\">Standard version<\/h3>\n        <p>\n          The panel is designed for use with Illumina\u00ae and Ion Torrent&#x2122;\n          sequencers. The workflow is efficient and structured into two rounds\n          of multiplex PCR, with library preparation taking approximately\n          1.5 days.\n        <\/p>\n        <p>\n          It generally requires between 10 and 250 ng of RNA or total nucleic\n          acid (TNA), extracted from FFPE specimens or other clinical sources.\n        <\/p>\n      <\/article>\n\n      <article class=\"dlw-card\">\n        <h3 class=\"dlw-card-title\">HT version (high throughput)<\/h3>\n        <p>\n          The panel is optimised for high-throughput workflows and Illumina\u00ae\n          sequencers. It enables the simultaneous preparation of multiple\n          libraries in 96-well plates through a scalable and automatable\n          protocol that retains the sensitivity and accuracy of the standard\n          design.\n        <\/p>\n        <p>\n          An input amount of between 10 and 200 ng of RNA or TNA per specimen\n          is recommended, making it suitable for laboratories with a high\n          analytical workload.\n        <\/p>\n      <\/article>\n    <\/div>\n\n    <div class=\"dlw-image-wrap\">\n      <img decoding=\"async\"\n        class=\"dlw-image\"\n        src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/08\/Imagen3-2.webp\"\n        alt=\"FUSIONPlex Sarcoma v2 workflow\"\n        loading=\"lazy\"\n        onclick=\"openDlwModal(this.src, this.alt)\"\n      >\n    <\/div>\n  <\/section>\n\n  <section class=\"dlw-section\">\n    <h2 class=\"dlw-title\">SOFTWARE<\/h2>\n\n    <p>\n      Archer&#x2122; Analysis is a comprehensive bioinformatics platform developed by\n      IDT for the automated analysis of NGS data generated with Archer\u00ae family\n      panels, including FUSIONPlex&#x2122;, VARIANTPlex&#x2122; and LIQUIDPlex&#x2122;. The\n      platform supports complete molecular analyses from DNA and RNA for\n      clinical and research applications.\n    <\/p>\n\n    <ul class=\"dlw-list\">\n      <li class=\"dlw-list-item\">\n        Compatible with data generated on Illumina\u00ae platforms for\n        FUSIONPlex&#x2122;, VARIANTPlex&#x2122; and LIQUIDPlex&#x2122;, and on Ion Torrent&#x2122;\n        platforms for FUSIONPlex&#x2122;.\n      <\/li>\n\n      <li class=\"dlw-list-item\">\n        Includes optimised analytical pipelines. For FUSIONPlex&#x2122;, it detects\n        known and de novo gene fusions, splicing variants, exon-skipping events\n        and RNA expression.\n      <\/li>\n\n      <li class=\"dlw-list-item\">\n        Provides an intuitive, secure web interface with filters, automated\n        reports and annotations from databases including ClinVar, COSMIC and\n        Ensembl.\n      <\/li>\n\n      <li class=\"dlw-list-item\">\n        Supports systematic error correction, read deduplication and accurate\n        quantification of original molecules using molecular barcode\n        information.\n      <\/li>\n\n      <li class=\"dlw-list-item\">\n        Designed for regulated environments, with traceability,\n        reproducibility and scalability. It may be used in RUO or CE-IVD\n        workflows according to the panel configuration.\n      <\/li>\n    <\/ul>\n  <\/section>\n\n  <section class=\"dlw-section\">\n    <h2 class=\"dlw-title\">SPECIFICATIONS<\/h2>\n\n    <div class=\"dlw-highlight\">\n      <strong>Broad detection capability:<\/strong>\n      known and de novo fusions, splicing variants, SNVs, insertions,\n      deletions and RNA expression.\n    <\/div>\n\n    <div class=\"dlw-image-wrap\">\n      <img decoding=\"async\"\n        class=\"dlw-image\"\n        src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/08\/Imagen4-2.webp\"\n        alt=\"Technical specifications of the FUSIONPlex Sarcoma v2 panel\"\n        loading=\"lazy\"\n        onclick=\"openDlwModal(this.src, this.alt)\"\n      >\n    <\/div>\n\n    <div class=\"dlw-feature-grid\">\n      <div class=\"dlw-feature\">\n        <strong>AMP&#x2122; technology<\/strong>\n        Improves sensitivity and accuracy in the detection of genetic\n        alterations.\n      <\/div>\n\n      <div class=\"dlw-feature\">\n        <strong>Optimised for FFPE<\/strong>\n        Suitable for low-quality specimens.\n      <\/div>\n\n      <div class=\"dlw-feature\">\n        <strong>Starting amount of genetic material<\/strong>\n        Requires only 10 ng of RNA.\n      <\/div>\n\n      <div class=\"dlw-feature\">\n        <strong>Universal compatibility<\/strong>\n        Works with Illumina\u00ae and Ion Torrent&#x2122; sequencers.\n      <\/div>\n\n      <div class=\"dlw-feature\">\n        <strong>Bioinformatics support<\/strong>\n        Includes the Archer\u00ae Analysis platform for data analysis and\n        visualisation.\n      <\/div>\n    <\/div>\n  <\/section>\n\n  <section class=\"dlw-section\">\n    <h2 class=\"dlw-title\">COMPLETE GENE LIST<\/h2>\n\n    <div class=\"dlw-image-wrap\">\n      <img decoding=\"async\"\n        class=\"dlw-image\"\n        src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2026\/08\/Imagen5-1.webp\"\n        alt=\"Complete gene list for the FUSIONPlex Sarcoma v2 panel\"\n        loading=\"lazy\"\n        onclick=\"openDlwModal(this.src, this.alt)\"\n      >\n    <\/div>\n  <\/section>\n<\/div>\n\n<div\n  id=\"dlw-image-modal\"\n  class=\"dlw-modal\"\n  role=\"dialog\"\n  aria-modal=\"true\"\n  aria-label=\"Expanded product image\"\n  onclick=\"closeDlwModalOnBackdrop(event)\"\n>\n  <button\n    class=\"dlw-modal-close\"\n    type=\"button\"\n    aria-label=\"Close expanded image\"\n    onclick=\"closeDlwModal()\"\n  >\n    &times;\n  <\/button>\n\n  <img decoding=\"async\"\n    id=\"dlw-modal-image\"\n    class=\"dlw-modal-image\"\n    src=\"\"\n    alt=\"\"\n  >\n<\/div>\n\n<script>\n  function openDlwModal(imageSource, imageAlt) {\n    var modal = document.getElementById(\"dlw-image-modal\");\n    var modalImage = document.getElementById(\"dlw-modal-image\");\n\n    if (!modal || !modalImage) {\n      return;\n    }\n\n    modalImage.src = imageSource;\n    modalImage.alt = imageAlt || \"Expanded product image\";\n    modal.classList.add(\"dlw-modal-open\");\n    document.body.style.overflow = \"hidden\";\n  }\n\n  function closeDlwModal() {\n    var modal = document.getElementById(\"dlw-image-modal\");\n    var modalImage = document.getElementById(\"dlw-modal-image\");\n\n    if (!modal) {\n      return;\n    }\n\n    modal.classList.remove(\"dlw-modal-open\");\n    document.body.style.overflow = \"\";\n\n    if (modalImage) {\n      modalImage.src = \"\";\n      modalImage.alt = \"\";\n    }\n  }\n\n  function closeDlwModalOnBackdrop(event) {\n    if (event.target && event.target.id === \"dlw-image-modal\") {\n      closeDlwModal();\n    }\n  }\n\n  document.addEventListener(\"keydown\", function (event) {\n    if (event.key === \"Escape\") {\n      closeDlwModal();\n    }\n  });\n<\/script>\n","protected":false},"excerpt":{"rendered":"<p>NGS assays associated with soft tissue cancers.<\/p>\n","protected":false},"featured_media":7611,"template":"","tecnologias":[36],"marcas":[217],"familias":[460,470,347,464],"class_list":["post-8254","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-next-generation-sequencing-ngs","marcas-archer-dx-en","familias-fusions","familias-soft-tissues","familias-solid-tumor","familias-tumor-types"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/8254","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":1,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/8254\/revisions"}],"predecessor-version":[{"id":28272,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/8254\/revisions\/28272"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/7611"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=8254"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=8254"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=8254"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=8254"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}