{"id":9685,"date":"2022-10-17T13:23:04","date_gmt":"2022-10-17T13:23:04","guid":{"rendered":"https:\/\/www.dlongwood.com\/spot-it-2\/"},"modified":"2025-05-15T11:48:53","modified_gmt":"2025-05-15T11:48:53","slug":"spot-it","status":"publish","type":"productos","link":"https:\/\/www.dlongwood.com\/en\/product-catalog\/spot-it\/","title":{"rendered":"SPOT-it\u2122"},"content":{"rendered":"\n<p class=\"justificar\">Neonatal screening tests for severe combined immunodeficiency, X-linked agammaglobulinaemia or Bruton&#8217;s disease SPOT-it\u2122 neonatal screening tests offer a complete solution for the detection of several pathologies:<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter size-large\"><img decoding=\"async\" src=\"https:\/\/www.dlongwood.com\/wp-content\/uploads\/2022\/10\/SPOT-it\u2122en-1024x466.jpg\" alt=\"\" class=\"wp-image-13534\" title=\"\"><\/figure><\/div>\n\n\n\n<p><strong>Features<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>From Dried Blood Spot (DBS) to screening result<strong> within 3 hours.<\/strong><\/li><li>Kit includes reagents for <strong>DNA extraction.<\/strong><\/li><li><strong>Ready-to-use plates pre-filled <\/strong>with reaction mix and samples to generate a standard curve.<\/li><li><strong>Just two pipetting steps <\/strong>for quick and easy handling.<\/li><li>Uses conventional <strong>3.2 mm <\/strong><strong>\u2300<\/strong><strong> DBS as input.<\/strong><\/li><li><strong>CE-IVD <\/strong>marked.<\/li><\/ul>\n\n\n\n<p><strong>4-step process<\/strong><\/p>\n\n\n\n<p>1. Punching of DBSs into SPOT-it\u2122 filter plate.<br>2. Rehydration of the samples<br>3. DNA elution<br>4. qPCR for amplification of TREC\/KREC\/<em>SMN1<\/em> mutation<\/p>\n\n\n\n<p><strong>SPOT-it\u2122 Analysis Software (CE-IVD)<\/strong><\/p>\n\n\n\n<p class=\"justificar\">The SPOT-it\u2122 Analysis Software is designed to <strong>assist laboratory technicians in the qualitative visualisation of data<\/strong> from the qPCR thermal cycler software when using any of the SPOT-it\u2122 tests.<\/p>\n\n\n\n<p class=\"justificar\">The software <strong>checks all run quality parameters<\/strong> (e.g. deviation from standard curve, value obtained by positive controls and that of internal controls) and assigns the labels &#8220;out of range&#8221;, &#8220;within range&#8221; or &#8220;inconclusive&#8221; to each sample for TRECs and KRECs and &#8220;positive&#8221;, &#8220;negative&#8221; or &#8220;inconclusive&#8221; for the <em>SMN1<\/em> gene.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Neonatal screening tests for severe combined immunodeficiency, X-linked agammaglobulinaemia or Bruton&#8217;s disease and spinal muscular atrophy.<\/p>\n","protected":false},"featured_media":9683,"template":"","tecnologias":[275],"marcas":[247],"familias":[284,283,451,289,303],"class_list":["post-9685","productos","type-productos","status-publish","has-post-thumbnail","hentry","tecnologias-rt-pcr-en","marcas-immunoivd-en","familias-immunology","familias-molecular-genetics","familias-newborn-screening-en","familias-newborn-screening","familias-reproduction"],"_links":{"self":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/9685","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos"}],"about":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/types\/productos"}],"version-history":[{"count":0,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/productos\/9685\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media\/9683"}],"wp:attachment":[{"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/media?parent=9685"}],"wp:term":[{"taxonomy":"tecnologias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/tecnologias?post=9685"},{"taxonomy":"marcas","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/marcas?post=9685"},{"taxonomy":"familias","embeddable":true,"href":"https:\/\/www.dlongwood.com\/en\/wp-json\/wp\/v2\/familias?post=9685"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}