SRSLY NGS Library Prep Kit

Introduction

The SRSLY NGS Library Prep Kit from ClaretBio offers an efficient and simplified solution for library preparation using a technology based on single-stranded DNA through a ligation method. Unlike traditional library preparations, which are based on double-stranded DNA, SRSLY kits allow the entire DNA of the initial sample to be transformed into a sequenceable library, even in samples with minimal amounts of DNA (250 pg) and degraded DNA (kits compatible with paraffin-extracted DNA, ancient DNA…). Additionally, this kit enables the generation of complex libraries in a very short time of ~2.5 hours.

Detailed Description

The SRSLY kit provides three bead purification options (retention of small, moderate, and large fragments), adapting to different types and sizes of starting DNA. Available in a modular format, it offers maximum flexibility for the user in the choice of base kit, reaction volume, bead purification, and the addition of unique molecular indices (UMI) or dual indices (UDI) during PCR amplification.

Key Aspects

• Fast and efficient: Preparation in 2.5 hours.
• Versatility: Suitable for low starting amounts of DNA (such as cfDNA) and degraded DNA (ancient DNA, paraffin-extracted DNA).
• Modularity: Allows customization according to project needs.
• Bead purification: Three options for different fragment sizes.
• UMI and UDI primers: For precise identification and reduction of PCR duplicates.

What the Kit includes

• Base kit for 24 or 96 reactions.
• UMI and UDI primer options.
• Bead purification modules.
• Detailed user manual and technical support.