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Human Platelet Antigens (HPA) Fluogene typing

CatalogHematology and blood bankBlood Group Genotyping (RBC)

HPA fluogene systems enables the detection of the antigens HPA-a y HPA–b

FluoGene is a unique method for molecular HPA-, RBC- (blood group) and HLA typing combining all advantages of the SSP–PCR with the speed of endpoint fluorescence detection.

The analysis is based on a specifically modified TaqMan® probe system by inno-train. There are two options for detection: the FluoVista analyzer or the FluoQube – inno-train’s real-time device.

  • Up to four fluorophores are detected by the FluoVista® analyzer and an integrated data evaluation is realized by the FluoGene® software.
  • FluoQube® combines the FluoGene® method with real-time PCR. This system allows amplification and fluorescence reading in one system. The real-time data are automatically imported into the FluoGene® Software for result evaluation.

Human Platelet Antigens (HPA) are glycoproteins expressed on the membrane of platelets. Antibodies against the Human Platelet Antigens can cause immune reactions in the recipient´s bood leading to the lysis of the transfused platelets.

HPA-Fluogene Kit  enables the detection of the two variants (a and b) of the platelet antigens HPA-1, HPA-2, HPA-3, HPA-4, HPA-5, HPA-6, HPA-9 and HPA-15 . The software indicates the alleles present in each sample.

All FluoGene kits are CE marked in accordance with the IVD Directive 98/79/EC  and manufactured and tested under a quality system in compliance with DIN EN ISO 13485.

ADVANTAGES

  • PCR-SSP method without gel electrophoresis within 90 minutes
  • no hybridization and washing steps
  • display of typing results immediately after the post-read
  • no risk of post-PCR contaminations
  • low DNA consumption
  • user-friendly software solution, fully-automated calculation of results
  • same system for HLA-, RBC- and HPA
  • use of patented fluorescent dyes and quencher

Documentation

ArticleTest/PlateTests/Kit
HPA-FluoGene6/148/10
HPA-FluoGene HPA-1 a/b Screen1248

Public Documentation

  • Innotrian-FluoGene-ficha
  • Innotrain-FluoGene-Kits

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    Product families

    • Molecular Genetics
      • Prenatal Diagnosis
      • Triplet Expansion
      • Pharmacogenomics
      • Cystic Fibrosis
      • Other – Genetics
      • Genetic Panels
      • Reproduction
    • Hematology and blood bank
      • Platelet Antibody Testing
      • Cryopreservation
      • Blood Group Genotyping (RBC)
      • Other – Hematology
      • Thrombophilias and Hemochromatosis
    • Immunology and transplantation
      • Alloantibodies Detection
      • HLA-associated diseases
      • Immunosequencing
      • Other – Immunology
      • Chimerism
      • Immune response and biomarkers
      • HLA Typing
    • Microbiology
      • Virus
      • Fungus
      • DNA/RNA extraction
      • CMI Quick-Sepsis
      • Metatranscriptomics
    • Oncology
      • Oncohematology
      • Solid Tumor
    • Preanalytics
      • Sampling and Extraction
      • Blood collection
      • Sample transportation

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