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RBC FluoGene - Blood group typing

CatalogHematology and blood bankBlood Group Genotyping (RBC)

Molecular RBC-typing based on a specifically modified TaqMan® probe system
innotrain fluogene

FluoGene is a unique method for molecular blood group (RBC), HPA- and HLA-typing combining all advantages of the SSP-PCR with the speed of endpoint fluorescence detection.

The analysis is based on a specifically modified TaqMan® probe system by inno-train. There are two options for detection: the FluoVista analyzer or the FluoQube – inno-train’s real-time device.

  • Up to four fluorophores are detected by the FluoVista® analyzer and an integrated data evaluation is realized by the FluoGene® software.
  • FluoQube® combines the FluoGene® method with real-time PCR. This system allows amplification and fluorescence reading in one system. The real-time data are automatically imported into the FluoGene® Software for result evaluation.

inno-train's RBC-Fluogene Kits enable the molecular typing of several blood groups:

  • RBC-FluoGene ABO basic y ABO plus: A1, A2, B, 01 y 02
  • RBC-FluoGene vERYfy: RHD, RHCE, MNS, Kell, Kidd, Duffy and Dombrock
  • RBC-Fluogene vERYfy eXtend: RHD, RHCE, KEL, JK, FY, MNS, DO, LU, YT, DI, VEL, CO and KN in a single assay, including new primers for RHD*01W.59 (D 59 weak) detection
  • RBC-FluoGene CDE: RHD and RHCE C, c, E, e, CW. Besides, two new alleles (RhCE-733C>G, RhCE-1006G>T) enabling to predict VS and S phenotypes.
  • ​RBC-FluoGene D weak/variant: more frequent weak D alleles (1, 2, 3, 4.0/ 4.1, 4.2, 5, 11, 14, 15, 17, K409K, IVS3+1G>A)
  • RBC-FluoGene KKD: alleles Kell, Kidd and Duffy
  • RBC-FluoGene Rare: several rare blood alleles DI1, DI2, DI3, DI4, YT1, YT2, LU1, LU2, KEL3, KEL4, KEL6, KEL7, CO1, CO2, KN1, KN2
  • RBC-FluoGene D-Screen: 3/5 and 10 RHD exons screening in a single reaction
  • RBC-FluoGene MNS: MNS group alleles. Now alleles GYPB will further included for the detection of the two common U+var alleles: U+var(NY) and U+var(P2) 
  • RBC-FluoGene Vel-Screen: Vel group screening. Vel+ and Vel- genotyping

ADVANTAGES:

  • PCR-SSP method without gel electrophoresis within 90 minutes
  • no hybridization and washing steps
  • display of typing results immediately after the post-read
  • no risk of post-PCR contaminations
  • low DNA consumption
  • user-friendly software solution, fully-automated calculation of results
  • same system for HLA-, RBC- and HPA
  • use of patented fluorescent dyes and quencher

Documentation

ProductReactions/testTest/PlateTest/Kit
RBC-FluoGene ABO basic 17 4 / 1 40 / 10174/140/10
RBC-FluoGene vERYfy 32 3 / 1 30 / 10323/130/10
RBC-FluoGene CDE 24 4 / 1 40 / 10244/140/10
RBC-FluoGene D weak/variant 16 6 / 1 48 / 10166/148/10
RBC-FluoGene Rare 32 3 / 1 30 / 10323/130/10
RBC-FluoGene D-Screen 1 12 4811248
RBC-FluoGene Vel-Screen 2 12 4821248
RBC-FluoGene MNS 7 1 107110
RBC-FluoGene KKD 16 1 1016110
RBC-FluoGene vERYfy eXtend 96 1 1096110

Public Documentation

  • IT FluoGene_ES v2

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    Product families

    • Molecular Genetics
      • Prenatal Diagnosis
      • Triplet Expansion
      • Pharmacogenomics
      • Cystic Fibrosis
      • Other – Genetics
      • Genetic Panels
      • Reproduction
    • Hematology and blood bank
      • Platelet Antibody Testing
      • Cryopreservation
      • Blood Group Genotyping (RBC)
      • Other – Hematology
      • Thrombophilias and Hemochromatosis
    • Immunology and transplantation
      • Alloantibodies Detection
      • HLA-associated diseases
      • Immunosequencing
      • Other – Immunology
      • Chimerism
      • Immune response and biomarkers
      • HLA Typing
    • Microbiology
      • Virus
      • Fungus
      • DNA/RNA extraction
      • CMI Quick-Sepsis
      • Metatranscriptomics
    • Oncology
      • Oncohematology
      • Solid Tumor
    • Preanalytics
      • Sampling and Extraction
      • Blood collection
      • Sample transportation

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