Bruker’s nCounter® is a molecular analysis platform that enables the direct, multiplexed quantification of up to ~800 targets (RNA or proteins) in a single reaction, without the need for PCR amplification or cDNA conversion.
Key Features
How does it work?
The nCounter assay directly quantifies RNA, miRNA, or protein molecules by hybridizing two specific probes: a capture probe that immobilizes the sample in a cartridge and a reporter probe with a unique fluorescent barcode for each target, allowing for individual digital counting of the complexes without the need for amplification.
Subsequently, the probe-target complexes are purified, immobilized, and aligned for signal scanning using an automated fluorescence microscope. The generated data (CSV files) is then analyzed using Bruker’s free nSolver™ analysis software.
The nCounter workflow incorporates walk-away automation and requires less than 15 minutes of hands-on time. Most protocols require only 4 pipetting steps and generate results in less than 24 hours.
Applications:
Available panels
nCounter offers gene (gray) and protein (orange) expression panels carefully designed by experts across a wide variety of research areas. All panels are created in collaboration with specialists in each field and are regularly updated. Click on each icon for more information about these panels:
Oncology
Immunology
Infectious diseases
Neuroscience
Cell and Gene Therapy
Gene signatures
Cardiovascular disease
Proteins
Data analysis
nSolver with the Advanced Analysis plugin allows for deep analysis of cell populations and biological pathways. In addition, it can quantify individual cell types using gene signatures, currently identifying 14 immune cell types as well as neuroscience signatures. For more information, please refer to the following link: nSolver Data Analysis Support | NanoString
Gene list
To view the list of genes included in each panel, please click on the following link: nCounter RLFs, Probe Annotation Files, and Gene Lists | NanoString
