High-performance exome for the enrichment of human coding regions, with updated coverage of RefSeq, CCDS and GENCODE, including the TERT promoter and difficult-to-capture exons.
3.- Detailed Description
Operating principle
SureSelect Human All Exon V8 is based on NGS library preparation and capture enrichment of target regions of the human exome.
The workflow is compatible with Agilent SureSelect systems and can be integrated into manual or automated protocols, depending on the laboratory configuration.
Clinical applications
Product intended for laboratories performing exome sequencing in genomic research, clinical genetics or molecular biology projects, especially when broad and uniform coverage of human coding regions is required.
Benefits
Complete and updated coverage of coding regions from RefSeq, CCDS and GENCODE, including the TERT promoter and difficult-to-capture exons.
Probe design based on machine learning to improve coverage, efficiency and uniformity, including difficult-to-capture exons and the TERT promoter.
Efficient design size, aimed at reducing sequencing requirements and costs per sample.
Automation compatibility with the Bravo Automated Liquid Handling Platform and Magnis NGS Prep System.
Results or key indicators
Panel coverage: target region of 35.1 Mb and design size of 41.6 Mb.
Target sequence coverage in 8-plex captures: 96% of bases ≥20X, 93% ≥30X, 86% ≥40X and 74% ≥50X, with a median read coverage of 63X.
Uniform distribution of coverage across the GC content spectrum.
Coverage of clinical interest:
100% of the coding content of CCDS, GENCODE and RefSeq.
179,645 RefSeq exons ≥20X and 14,168 RefSeq genes ≥20X; in ACMG 59 genes, 1,009 exons ≥20X.
Genes associated with rare diseases: 6,003 exons ≥20X, with 24% more genes completely covered at ≥20X
Technology used
The NGS workflow is based on library preparation and capture enrichment using Agilent SureSelect systems.
Possibility of integration into SureSelectXT HS2, SureSelectXT HS, SureSelectXT Low Input, SureSelectQXT and SureSelectXT legacy workflows
Automation on the Agilent Bravo Automated Liquid Handling platform and Agilent Magnis NGS Prep system.
Intended audience/user
The product is intended for genetics and molecular biology laboratories that require a scalable whole-exome workflow compatible with automation.
Considerations or limitations
RUO label (For Research Use Only)
4.- Key Aspects
✓
Human exome with a target region of 35.1 Mb and an efficient design of 41.6 Mb.
✓
Updated coverage of coding regions from RefSeq, CCDS and GENCODE.
✓
Includes the TERT promoter and difficult-to-capture exons.
✓
Probe design based on machine learning and an optimised manufacturing process.
✓
Uniform coverage across the GC spectrum, supporting the representation of GC-rich and GC-poor regions.
✓
High enrichment performance, with improved sequencing efficiency under the conditions evaluated by Agilent.
✓
Compatible with manual and automated workflows, including Bravo and Magnis.
5.- Presentation Details
Main formats indicated for SureSelect Human All Exon V8:
Compatible with rapid hybridisation
| Product |
16 Rxns |
96 Rxns |
96 Rxns Auto |
|
SureSelect XT HS Human All Exon V8
|
5191-6873 |
5191-6874 |
5191-6875 |
*Pre-Capture Formats
| Product |
2 Hybs |
12 Hybs |
12 Hybs Auto |
|
SureSelect XT HS PreCap Human All Exon V8
|
5191-6876 |
5191-6877 |
5191-6878 |
Compatible with overnight hybridisation
| Product |
16 Rxns |
96 Rxns |
96 Rxns Auto |
|
SureSelect XT Human All Exon V8
|
5191-6879 |
5191-6891 |
5191-6892 |
*Pre-Capture Formats
| Product |
2 Hybs |
12 Hybs |
12 Hybs Auto |
|
SureSelect XT PreCap Human All Exon V8
|
5280-0032 |
5280-0033 |
5280-0034 |
Compatible with Magnis NGS Prep System
| Product |
32 Rxns |
96 Rxns |
|
Magnis SSEL XT HS Human All Exon V8, Rev B
|
G9772C |
G9772D |
|
Magnis SSEL XT HS2 Human All Exon V8, Rev B
|
G9774A |
G9774B |
