Platinum® y Platinum® Pro

Platinum® and Platinum® Pro

Platinum® and Platinum® Pro, developed by Quantum-Si, are innovative protein sequencing platforms based on single molecule technology. Designed for research laboratories, this comprehensive solution allows direct and easy preparation, sequencing and analysis of proteins, without the need for advanced bioinformatics knowledge.

Clinical and research applications include protein and variant identification, post-translational modification (PTM) analysis, antibody characterization, protein barcoding and biomarker searching. Its direct focus on proteins avoids the need for inferences from genomic or transcriptomic data, providing a more accurate view of the actual protein state.

How does it work?

The principle of operation of Platinum® and Platinum® Pro is based on the individual sequencing of peptides attached by Lys-C to the wells of the sequencing chip. Using aminopeptidases that successively cleave the N-terminal amino acids (NAA), the system captures the fluorescent signal emitted during each recognizer binding event. This signal generates a kinetic signature specific to each amino acid, which allows it to be identified. The process is repeated until the peptide is completely sequenced. The data obtained are automatically transferred to the Platinum analysis software, which provides results at the individual molecule level, facilitating a clear and accurate interpretation.

Key aspects

• Direct protein sequencing with single amino acid resolution.
• Single molecule technology in a benchtop format.
• Easy workflow with less than 3 hours of manual intervention.
• No bioinformatics expertise or dedicated lab space required.
• Applications in identification of proteins, variants, PTMs, antibodies and biomarkers.
• Automated analysis software with cloud access.
• Comprehensive kits for library preparation, sequencing and control.

Designed for your Laboratory

The NGPS system is designed to be scalable and accessible. Its desktop format, low handling time (<3 hours) and ease of integration into existing workflows make it ideal for a variety of laboratory environments. In addition, it requires no dedicated space or specialized technical expertise, democratizing access to high-resolution protein sequencing.

The technology used integrates library preparation and sequencing kits containing all the necessary reagents, including enzymes and specific recognizers, as well as semiconductor chips for peptide immobilization. Connectivity to cloud-based software facilitates collaborative analysis and the availability of continuous updates.

Kits

1. Library Preparation Kit, V2, Lys-C

Kit for the digestion, functionalization and immobilization of proteins, an essential step prior to protein sequencing on the Platinum® platform.

Key Aspects

• Compatible with 6–86 kDa proteins.
• Allows input of 100–500 pmol per sample.
• Preparation process with less than 2 hours of intervention.
• High identification performance even at low concentrations.
• Direct integration into the Platinum® workflow.

3. Barcoding Kit

Multiplexed protein barcode sequencing kit, ideal for relative expression studies, drug development and functional characterization. It allows multiplexed functional studies of up to eight protein variants in parallel, with single molecule level resolution.

Key Features

• Ability to analyze up to 8 barcodes per sample.
• Relative quantification in dynamic range up to 10-fold.
• Validated sensitivity up to 50 fmol (single), 400 fmol (8-plex).
• Fast workflow: <1 hour manual intervention.
• Ideal for gene therapy research, mRNA vaccines, recombinant proteins.