RNA-based next-generation sequencing solution for the analytically sensitive identification of known and novel gene fusions, splicing variants, exon-skipping events, SNVs/Indels and gene expression in haematological malignancy research.
FUSIONPlex Pan-Heme v2 provides broad coverage of 218 genes associated with haematological malignancies, while FUSIONPlex Heme v3 provides more focused coverage of 121 genes targeting alterations frequently found in leukaemia while retaining markers relevant to other haematological diseases.
Detailed Description
Operating Principle
The Archer FUSIONPlex Heme v3 & Pan-Heme v2 solutions are based on Anchored Multiplex PCR (AMP™) chemistry, a proprietary multiplex PCR strategy that uses unidirectional gene-specific primers to enrich target regions and enable the detection of known and unknown alterations. The adapters incorporate molecular barcodes (MBCs, Molecular Barcodes) and sample indices, enabling multiplexed analysis, read deduplication, error correction and high-confidence variant calling.
The approach is designed for RNA fusion analysis, enabling expressed fusion transcripts to be captured and avoiding the technical and economic difficulties associated with sequencing large repetitive intronic regions in DNA-based assays. In combination with de novo assembly and Archer Analysis software, FUSIONPlex enables the sensitive identification of known and novel fusions, including fusions with previously unidentified partners (fusion-agnostic).
Benefits
Technology Used
Intended Audience/User
Molecular diagnostics, haematology, haemato-oncology and haematological malignancy genomics laboratories requiring the characterisation of fusions, splicing alterations, SNVs/Indels and gene expression using NGS.
The protocols are intended for research applications (RUO) and must be used together with Archer reagent kits and the corresponding sequencing kits for Illumina or Ion Torrent platforms.
Key Features
Presentation Details
FUSIONPlex/ FUSIONPlex-HT Pan-Heme v2 Panel
FUSIONPlex/ FUSIONPlex-HT Heme v3 Panel
FUSIONPlex Heme v3 and PanHeme v2 can be used as standalone panels, combined with other Archer research panels or customised for specific requirements. It can also be combined with VARIANTPlex™ Myeloid v2 for comprehensive genomic characterisation and with IMMUNOVerse™ BCR for immune profiling.
