GenDx-ExoSAP is an enzymatic product designed to remove primers and nucleotides from PCR products, creating a DNA sample ready for sequencing.
Exonuclease I (ExoI) degrades single-stranded DNA primers.
Shrimp Alkaline Phosphatase (SAP) dephosphorylates nucleotides.
Both enzymes are degraded by heat inactivation.
After preparing the GenDx-ExoSAP reaction mix, only 2 μl of GenDx-ExoSAP reaction mix are added to each of the PCR products.