Ultra-fast solution for high-resolution HLA typing, featuring single-tube library preparation and sequencing using Oxford Nanopore technology.
Detailed Description
NGS-Turbo® is an innovative platform developed by GenDx for high-resolution HLA typing, specially designed for environments where speed and workflow simplicity are critical. This system combines full genomic amplification of 11 HLA loci with a single-tube library preparation procedure, followed by sequencing using nanopore technology (Oxford Nanopore Technologies).
The system consists of three main components:
1. NGS-TurboAmp: enables simultaneous amplification of 11 HLA loci (HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1, and -DPB1) using a whole-gene approach. Primers are provided pre-mixed with enzymes, buffer, and water, which simplifies the process, as it only requires adding the DNA sample. Amplification is completed in approximately 95 minutes.
2. NGS-TurboPrep: facilitates library preparation in 30 minutes without requiring DNA fragmentation, preserving the full length of the amplicons. Incubations are performed at room temperature, and the workflow can be paused at any time, providing great flexibility. The protocol is based on a streamlined sequence of steps: end prep, adapter ligation, magnetic bead cleanup, and elution.
3. Sequencing with Oxford Nanopore: the system is compatible with MinION Flow Cells (FLO-MIN114) and allows sequencing to begin immediately after sample preparation. Sequencing time can be as short as 20 minutes, depending on the device used.
Once sequencing is complete, data is processed using NGSengine®-Turbo, GenDx’s dedicated software that analyzes results in just 5 minutes. The interface is optimized for clear visualization, minimizing the required inspection time and facilitating direct, useful reporting.
NGS-Turbo® represents a comprehensive, fast, and accurate solution for histocompatibility laboratories and transplant centers requiring results in record time without compromising quality.
Key Features
High-resolution HLA typing in under 3 hours.
Coverage of 11 HLA loci through whole-gene amplification.
Single-tube library preparation with no fragmentation required.
Streamlined protocol with room temperature incubations.
Compatible with Oxford Nanopore sequencers (MinION Flow Cells).
Fast and reliable data analysis with NGSengine®-Turbo.
Ideal for clinical applications where speed is critical.
Ordering Information
Main components: NGS-TurboAmp, NGS-TurboPrep, MinION Flow Cell (FLO-MIN114), and NGSengine®-Turbo software.Single-tube preparation format.No fragmentation or complex intermediate steps required.Compatible with Oxford Nanopore Technologies sequencing devices.Manufacturer contact: GenDx, Utrecht (The Netherlands) – www.gendx.com
Area:
High resolution HLA typing, Immunology, Pre transplantation
