Multiplex PCR kit platform for the rapid and direct detection of major respiratory pathogens. It allows simultaneous analysis of different viruses and bacteria directly from swab or saliva samples, without the need for nucleic acid extraction.
1. Product description
The RealAccurate® Quadruplex platform is an advanced molecular diagnostic solution consisting of a line of multiplex PCR kits for the rapid and differential identification of the causative agents of respiratory tract infections. The main advantage of this product family is its simplified workflow: it allows samples to be analysed directly after a brief heat incubation (99 °C for 10 minutes), completely eliminating the lengthy nucleic acid extraction step. This drastically reduces the total time to results to less than 2 hours, optimising clinical decision‑making in high‑pressure healthcare situations.
2. Kits included in the product family
RA Quadruplex SARS-CoV-2 PCR Kit (PF0971C-R): Simultaneous detection and confirmation of the SARS‑CoV‑2 N and RdRp genes in independent fluorescence channels.
RA Quadruplex Influenza PCR Kit (PF0970-R): Detection and differentiation of Influenza A, Influenza B and Influenza A (H1N1)pdm09.
RA Quadruplex Corona PCR Kit (PF0971-R): Detection and differentiation of human seasonal Coronaviruses 229E, OC43 and NL63/HKU1.
RA Quadruplex Parainfluenza PCR Kit (PF0972-R): Detection and differentiation of Parainfluenza virus types 1, 2, 3 and 4.
RA Quadruplex RSV/hMPV PCR Kit (PF0973-R): Detection and differentiation of Respiratory Syncytial Virus A (RSVA), RSVB and human Metapneumovirus (hMPV).
RA Quadruplex Adeno/Boca/Rhino/Entero PCR Kit (PF0974-R): Partial detection and differentiation of Adenovirus, Bocavirus, Rhinovirus and Enterovirus.
RA Quadruplex Bordetella PCR Kit (PF0975-R): Detection and diagnosis of Bordetella pertussis.
RA Quadruplex Atypical bacteria PCR Kit (PF0966-R): Selective detection of atypical respiratory bacteria.
3. Key advantages and features
Direct detection without extraction: Thanks to its robust formulation, it allows direct processing of nasopharyngeal swab samples (in VTM, eSwab, 0.9% NaCl media) and saliva after a simple heat treatment, saving time, reagents and consumables.
High sensitivity and specificity: Clinically validated and tested against external quality control panels (QCMD). No cross‑reactivity with other common respiratory pathogens.
Safety and quality control: Each kit contains an internal control (IC — MS2 bacteriophage) to monitor possible PCR inhibition, plus ready‑to‑use positive (PC) and negative (NC) controls.
Maximum compatibility: Fully optimised and validated on the most common real‑time thermal cyclers in laboratories (Roche, QIAGEN, Bio‑Rad, Thermo Fisher, BMS, IT‑IS).
Professional use: Designed and manufactured under the strict EN ISO 13485:2016 quality standard for exclusive use in clinical diagnostic laboratories.
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