This is a comprehensive solution for human whole‑genome sequencing that provides a thorough and high‑resolution view, optimising variant detection and genetic research. Its workflow enables the generation of high‑precision libraries with uniform coverage.
Detailed description
Key features
Illumina DNA PCR-Free Prep, Tagmentation is designed to offer a fast, flexible, and high‑throughput workflow for whole‑genome sequencing applications. The solution combines bead‑based tagmentation technology with PCR‑free chemistry, reducing amplification bias and improving coverage uniformity in complex genomic regions or those with irregular base composition.
The protocol is compatible with different input types, allowing the assay to be adapted to various sample types and experimental needs.
Clinical applications
Illumina DNA PCR-Free Prep, Tagmentation is primarily aimed at whole‑genome sequencing applications, especially when high coverage uniformity and variant calling accuracy are required. Possible applications include, for example:
Research into genetic‑based rare diseases
Identification of variants in tumour vs. normal samples
De novo microbial genome assembly
Sensitive applications where PCR bias may compromise coverage or accuracy
Library preparation from samples such as blood, saliva, or dried blood spots
Benefits
Higher data quality and uniformity, thanks to a PCR‑free workflow combined with bead‑based tagmentation, which improves genomic coverage.
Reduction of amplification bias, especially in complex regions such as GC‑ or AT‑rich areas, repetitive regions, or hard‑to‑cover areas.
Simpler and faster protocol, with fewer preparation steps and shorter processing time (up to ~90 min for library preparation).
Reduced manual handling, by incorporating automatic normalisation and volume‑based library pooling.
More efficient sample processing, reducing the need for quantifications before and after library preparation.
Greater compatibility with automation, facilitating more robust, reproducible and scalable workflows.
Intended user / audience
Solution aimed at genomic research laboratories that require fast, robust and scalable NGS library preparation for whole‑genome sequencing and other sensitive applications. It is especially indicated for environments that need to reduce PCR bias, work with low DNA inputs, automate library preparation workflows and process sample batches efficiently.
Key features
PCR‑free NGS library preparation using bead‑based tagmentation.
Fast workflow: approximately 90 minutes from extracted genomic DNA.
Compatible with gDNA, blood, saliva, plasmids and dried blood spots.
Input DNA amount from 25 ng to 300 ng, with a maximum indicated of 2 µg.
Reduction of PCR‑induced bias.
Uniform coverage in human WGS applications.
Improved coverage in complex, GC‑ or AT‑rich regions.
Compatible with automation.
Up to 384 dual indexes for high‑productivity projects.
Presentation details
Illumina DNA PCR-Free Prep, Tagmentation (24 samples) — Catalog number: 20041794
Illumina DNA PCR-Free Prep, Tagmentation (96 samples) — Catalog number: 20041795
Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) — Catalog number: 20091654
Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) — Catalog number: 20091656
Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) — Catalog number: 20091658
Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) — Catalog number: 20091660
Illumina Lysis Reagent Kit — Catalog number: 20042221
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