Short description
The Tapestri® platform enables the simultaneous integration of three biological dimensions from the same individual cell: DNA, RNA and surface proteins. This unprecedented multi-omics capability provides a direct and functional view of how specific genetic alterations (genotype) shape transcriptional expression and protein behaviour (phenotype), without the need to reconcile data from separate platforms.
Conventional high-throughput sequencing technologies (Bulk NGS) provide an averaged view of cell populations. However, in complex diseases such as cancer, clonal evolution does not occur on average. Rare subclonal variations that drive therapeutic resistance, persistence and relapse are often masked by background noise.
The Mission Bio Tapestri® platform is the first and only solution on the market capable of performing large-scale targeted multi-omics at the single-cell level. By combining precision microfluidic instrumentation, advanced reagents and state-of-the-art bioinformatics tools, Tapestri® transforms complex samples of individual cells into high-resolution sequencing data, revealing cellular heterogeneity, clonal architecture and direct genotype-phenotype relationships without the need for bioinformatics inference.
Key Features
How does it work?
TapestriTM integrates into a next-generation sequencing (NGS) workflow, providing a complete solution from individual cells to sequencing data and its analysis. It covers everything from panel design or the selection of predesigned panels to bioinformatics analysis on the Tapestri Pipeline and Tapestri Insights platforms.
The solution incorporates Tapestri Pipeline and Tapestri Insights, bioinformatics tools optimised for the analysis of DNA and proteins at the single-cell level.
Unlike traditional one-step microfluidic systems, which limit the types of reagents that can interact with the cell, Tapestri® uses an exclusive two-step workflow that independently optimises each chemical reaction:
Available panels
The Tapestri® platform offers unprecedented versatility through its targeted panel architecture. Panels can be selected, combined and customised according to the needs and biological scope of the project, and are divided into three key analytes: DNA, surface proteins and RNA.
A. Single-Cell DNA Panels (SNVs and CNVs)
DNA panels are designed to interrogate single-nucleotide variants (SNVs) and copy-number variations (CNVs) with exceptional coverage and uniformity, typically using between 20 and 500 amplicons.
B. Surface Protein Panels (Immunophenotyping)
They enable the simultaneous quantification of cell membrane proteins using oligonucleotide-conjugated antibodies, providing unprecedented phenotypic resolution at the single-cell level.
C. Targeted RNA Panels (Single-Cell Targeted RNA)
This new analyte on the Tapestri® platform makes it possible to evaluate the functional gene-expression signature to complement single-cell DNA genotyping information.
D. Custom panels
Unlimited flexibility for precision medicine. Combine mutation markers (DNA) and surface proteins specifically designed for your clinical cohort, accelerating the discovery of rare clones and resistance mechanisms.
