Chimerism monitoring by Next Generation Sequencing (NGS) is the latest development in chimerism surveillance and replaces the less sensitive conventional STR (short tandem repeat) method because of its higher sensitivity.

NGStrack provides reliable chimerism data, at both high and low percentages. Furthermore, the short workflow with only 1.5 hours hands-on time provides quick and easy results.

Distribucion de los marcadores a lo largo del genoma 1

The NGStrack reagents consist of a set of 34 indel markers covering 18 chromosomes, which allow to distinguish between donor and recipient, based on inserted or deleted DNA fragments. The kit also includes an X/Y marker..

NGStrack can be combined with NGSgo HLA samples on the same flow cell. If you are already using chimerism monitoring by qPCR (KMRtype, KMRtrack, and AlleleSEQR) and you want to combine or continue with NGS, the markers overlap so you can have the same informative

TRKengine

TRKengine software interprets the informativity of the markers and quantifies the chimerism percentage in post-samples. Its use as data management system allows for insightful chimerism monitoring and trending over time.


NGStrack reagents and TRKengine software are compatible with Illumina sequencing platforms. As such, NGStrack can easily be included in our renowned NGSgo® workflow that has been successfully used in HLA labs for many years.

Benefits

  • Only 1.5 hours of hands-on time
  • 2-10 times more sensitive than STR
  • Pre-sample needs to be tested only once

Area:

Bone marrow (chimerism), Chimerism (HSCT), Immunology, Oncohematology

Brand:

Documents:

Consult our experts

Google reCaptcha: Invalid site key.

Related products

HLA-KMR

Additional information in the monitoring of chimerism for the loss of HLA in a relapse.
GenDx
Real Time PCR (qPCR)

Franklin by QIAGEN for Somatic Analysis

Advanced cloud-based bioinformatics platform designed for the automated analysis, interpretation, and classification of somatic variants in oncology. Through an integrated environment, the system evaluates clinical evidence in real time, automates classification in accordance with AMP/ASCO/CAP guidelines, and provides access to currently active clinical trials. Detailed description Operating principle End-to-end cloud-based solution for the clinical interpretation…
Qiagen
Next Generation Sequencing (NGS)

SeqOne Genomics – Somatic

SeqOne is a bioinformatics platform for NGS data analysis in precision oncology, designed to detect, prioritise and interpret somatic variants and generate actionable clinical reports from raw sequencing data. It helps laboratories and healthcare professionals accelerate the interpretation of NGS data through an end-to-end workflow. Detailed description Operating principle SeqOne Genomics is a genomic data…
SeqOne
Next Generation Sequencing (NGS)

Nanotype® Mono

Optimized solution for high-resolution single-sample HLA typing using nanopore sequencing, ideal for environments requiring immediacy and flexibility. Detailed Description Nanotype® Mono is a compact solution from Werfen for high-resolution HLA typing based on nanopore sequencing technology (Oxford Nanopore Technologies), specially designed for the analysis of a single sample per run. It is ideal for laboratories…
Werfen
3rd Generation Sequencing (Long-read)