OVERVIEW
The FUSIONPlex® Sarcoma v2 panel is designed for RNA-based next-generation sequencing (NGS), using the patented Anchored Multiplex PCR (AMP™) technology.
AMP™ (Anchored Multiplex PCR) is a patented targeted enrichment platform for next-generation sequencing (NGS). It is based on the use of unidirectional gene-specific primers (GSPs) and molecular adapters with unique molecular barcodes (MBCs), which are ligated to the genetic material before amplification. This strategy enables efficient capture and amplification of DNA, RNA or cfDNA fragments, even in the presence of unknown mutations or highly fragmented sequences.
The anchored primer architecture supports a flexible design and reduces information loss during library preparation.
Combined with Archer™ Analysis software, AMP™ enables systematic error correction, read deduplication and accurate quantification of the original molecules, facilitating the detection of single-nucleotide variants, indels, gene fusions and copy-number alterations with high sensitivity and specificity.
RECOMMENDED TUMOUR TYPES
PROTOCOL
Standard version
The panel is designed for use with Illumina® and Ion Torrent™ sequencers. The workflow is efficient and structured into two rounds of multiplex PCR, with library preparation taking approximately 1.5 days.
It generally requires between 10 and 250 ng of RNA or total nucleic acid (TNA), extracted from FFPE specimens or other clinical sources.
HT version (high throughput)
The panel is optimised for high-throughput workflows and Illumina® sequencers. It enables the simultaneous preparation of multiple libraries in 96-well plates through a scalable and automatable protocol that retains the sensitivity and accuracy of the standard design.
An input amount of between 10 and 200 ng of RNA or TNA per specimen is recommended, making it suitable for laboratories with a high analytical workload.
SOFTWARE
Archer™ Analysis is a comprehensive bioinformatics platform developed by IDT for the automated analysis of NGS data generated with Archer® family panels, including FUSIONPlex™, VARIANTPlex™ and LIQUIDPlex™. The platform supports complete molecular analyses from DNA and RNA for clinical and research applications.
- Compatible with data generated on Illumina® platforms for FUSIONPlex™, VARIANTPlex™ and LIQUIDPlex™, and on Ion Torrent™ platforms for FUSIONPlex™.
- Includes optimised analytical pipelines. For FUSIONPlex™, it detects known and de novo gene fusions, splicing variants, exon-skipping events and RNA expression.
- Provides an intuitive, secure web interface with filters, automated reports and annotations from databases including ClinVar, COSMIC and Ensembl.
- Supports systematic error correction, read deduplication and accurate quantification of original molecules using molecular barcode information.
- Designed for regulated environments, with traceability, reproducibility and scalability. It may be used in RUO or CE-IVD workflows according to the panel configuration.
SPECIFICATIONS
COMPLETE GENE LIST
