KMRtype® / KMRtrack®

Comprehensive solution for chimerism study by qPCR, combining pre-transplant genetic typing kits and post-transplant quantitative monitoring.

Detailed Description

KMRtype® and KMRtrack® are complementary systems developed by GenDx for the characterization and monitoring of chimerism in transplant patients, using qPCR-based assays. The system allows everything from the identification of unique informative markers between recipient and donor, to the precise quantification of reference DNA in post-transplant samples.

KMRtype and KMRtrack KMRtype and KMRtrack Analysis

KMRtype® is a genetic typing kit that allows the selection of informative markers between recipient and donor DNA. Each mix contains three multiplexed primers and is used in pre-transplant settings. The kits are available in Core (30 assays in 10 mixes) and Extended (15 assays in 5 additional mixes) versions, allowing coverage of up to 45 assays in total. This phase is essential to identify which specific markers can be used effectively for monitoring.

KMRtrack and KMRengine

KMRtrack® is used for post-transplant monitoring using singleplex qPCR assays. Each marker selected in the typing phase is used to quantify the proportion of reference DNA (either from the recipient or the donor) in the samples, allowing precise monitoring of the patient’s chimeric status over time. The achieved limit of detection is 0.05%, depending on the amount of DNA used.

Both kits are optimized for use alongside the KMRengine® software, which guides experimental setup, manages qPCR plates, analyzes results, and automatically reports the chimerism percentage. The platform allows registering multiple samples, designing customized experiments, and visualizing data graphically and chronologically.

Performance and Validation

Validation studies showed 100% accuracy in typing and high intra- and inter-batch reproducibility in monitoring. Specificity was confirmed for all markers. Detection tests indicated that the system maintains robust performance with variable amounts of DNA, making it ideal for both clinical and experimental samples.

Important Considerations

Use with non-human DNA or cfDNA is not recommended. The use of at least two informative markers is recommended to avoid false results. Reagents are light-sensitive and must be stored at -20 °C. Heparinized samples should not be used, as they interfere with PCR. It is necessary to physically separate pre- and post-PCR areas to avoid cross-contamination.

Clinical Applications

This system is indicated for chimerism studies in the context of hematopoietic stem cell transplantation, with special utility in graft monitoring and early detection of relapse. Its sensitivity allows the detection of minimal amounts of donor or recipient-derived DNA, facilitating early therapeutic decisions.

Key Features

Complete system for chimerism typing and monitoring using qPCR.
Identification of specific informative markers for each donor-recipient pair.
Chimerism detection with sensitivity down to 0.05%.
Optimized protocols for genomic DNA from blood.
Automated analysis with KMRengine® software.
High inter-batch reproducibility and accuracy.
Compatible with multiple validated qPCR systems.

Ordering Information

KMRtype® RUO & CE-IVD: Core Mixes (30 assays, 24 samples) – Ref. 8341161 / Extended Mixes (15 assays, 24 samples) – Ref. 8341561 KMRtrack® RUO & CE-IVD: Core and Extended Kits, 48 reactions per assay – Ref. 8344162 and 8344562 KMRassay: Reference Assay (REF901) and Buffer & Enzyme – 288 reactions – Ref. 8343264 and 8343263 Versions also available as CE-IVD (see specific references in the IFU) For use with KMRengine® software (included in the GenDx platform) Store at -20 °C, protected from light

Area:

Bone marrow (chimerism), Immunology, Post transplantation

Brand:

Consult our experts

Google reCaptcha: Invalid site key.

Related products

Hedera Profiling RNA Test Panel

In‑house targeted enrichment NGS panel for RNA profiling from tissue samples (FFPE). It analyzes 43 genes using a splice‑aware probe design anchored to exon boundaries. Detects fusions in a partner-agnostic manner, complex splicing variants, and gene expression profiles in a single workflow. Detailed Description Principle of operation End‑to‑end solution based on hybrid capture enrichment chemistry…
HederaDx
Next Generation Sequencing (NGS)

Hedera Profiling ctDNA 3

In‑house targeted enrichment NGS panel exclusively for liquid biopsy (cfDNA). It analyzes 43 genes with ESCAT I and II clinical evidence. Includes library preparation through an optimized workflow (DNA‑only) for plasma samples. Detects SNVs, Indels, CNVs, fusions and 36 microsatellite instability (MSI) markers. Detailed Description Principle of operation End‑to‑end solution based on hybridisation and capture…
HederaDx
Next Generation Sequencing (NGS)

Hedera Profiling 1 FFPE Test Panel (HP1)

In‑house targeted enrichment NGS panel for pan‑cancer solid tumor profiling. It analyzes 115 genes (79 with complete CDS) with ESCAT I, II and III clinical evidence. Includes library preparation through a flexible workflow that allows processing FFPE and cfDNA samples together or separately. Detects SNVs, Indels, CNVs, fusions, structural variants and MSI. Detailed Description PRINCIPLE…
HederaDx
Next Generation Sequencing (NGS)

Sepsis Pathogenic Microorganism Detection Kit (Digital PCR)

In vitro diagnostic kit based on droplet digital PCR (dPCR) for the detection of pathogenic microorganisms associated with sepsis from DNA extracted from clinical samples. The kit allows identification and quantification of pathogens without requiring blood culture, covering 21 frequent agents in bloodstream infections. Detailed Description Principle of operation The Sepsis Pathogenic Microorganism Detection Kit…
RainSure
Digital PCR