Optimized solution for high-resolution single-sample HLA typing using nanopore sequencing, ideal for environments requiring immediacy and flexibility.
Detailed Description
Nanotype® Mono is a compact solution from Werfen for high-resolution HLA typing based on nanopore sequencing technology (Oxford Nanopore Technologies), specially designed for the analysis of a single sample per run. It is ideal for laboratories requiring immediate results or low-volume environments, without compromising accuracy or coverage.
This product maintains the core structure of Nanotype®, based on long-range PCR and a full-read library, but is specifically optimized for individual workflows, significantly reducing preparation and analysis times. In less than 5 hours, fully assembled and assigned high-resolution haplotypes can be obtained.
Nanotype® Mono covers the 11 clinically relevant HLA loci (HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1, -DPB1) and provides full-molecule sequences, preventing ambiguities and ensuring reliable, clear analysis.
The system is designed for use with ONT devices such as MinION, and employs a streamlined workflow including amplification, adapter ligation, and direct sequencer loading. Bioinformatics analysis is performed using the Nanotype® software, allowing allele assignment, manual review, visualization of covered regions, and automated reporting with clinical interpretation.
Key Features
