Taxonomic studies of fungi and other eukaryotes often rely on the nuclear ribosomal gene cluster, which includes the 18S gene. We offer an easy solution for the analysis of ITS1 regions for the characterization of fungi from various samples (culture, stool, sputum, skin, soil and water).

The protocol is based on PCR (“Polymerase Chain Reaction”) nucleic acid amplification of this internal transcribed spacer (ITS1) region located between the 18S and 5.8S rRNA genes.

  • Target: ITS1 region

Protocol diagram:

Imagen1 1

Other features:

  • 15,000- 100,000 reads/sample are the minimum recommended
  • Analysis software included
  • 96-reaction format

Area:

Fungus, Microbiology

Brand:

Documents:

Consult our experts

Google reCaptcha: Invalid site key.

Related products

Twist Custom Panels for NGS Applications

Twist custom panels are highly customisable solutions, allowing the exact selection of regions, genes, pathways or variants of interest. They are designed to offer high performance, flexibility and precision in all types of applications and starting samples. Detailed Description Operating principle: In practice, the use of custom panels makes it possible to overcome the limitations…
SOPHIA Genetics
Next Generation Sequencing (NGS)

STD-Finder® 2SMART

Multiplex qualitative real‑time PCR kit for the diagnosis of STDs Multiplex qualitative real‑time PCR kit for the rapid and specific diagnosis of sexually transmitted diseases (STDs), capable of detecting and differentiating 10 clinically relevant pathogens (including bacteria, viruses and parasites) from urine samples or genital, oropharyngeal or anal swabs. Product description The STD-Finder® 2SMART kit…
PathoFinder
Real Time PCR (qPCR)

RespiFinder® 2SMART

Multiplex qualitative real‑time PCR kit for the diagnosis of respiratory tract infections, allowing the simultaneous detection and differentiation of 22 respiratory pathogens (18 RNA viruses, 2 DNA viruses and 4 bacteria) in a single reaction. Product Description The RespiFinder® 2SMART kit is a qualitative in vitro diagnostic test designed to aid in the rapid and…
PathoFinder
Real Time PCR (qPCR)

MeningoFinder® 2SMART

Multiplex qualitative real‑time PCR kit for the rapid and specific diagnosis of meningitis and encephalitis, capable of detecting and differentiating 22 pathogens (4 RNA viruses, 8 DNA viruses, 8 bacteria and 2 fungi) in cerebrospinal fluid samples. Product Description The MeningoFinder® 2SMART kit is a high‑complexity qualitative in vitro diagnostic test that provides rapid identification…
PathoFinder
Real Time PCR (qPCR)