Multiplex real‑time PCR kits for the detection and differentiation of the most prevalent dermatophyte species in nail, skin, and hair samples.
Detailed Description
DermaGenius® kits are designed for the rapid and accurate detection of dermatophytic infections, providing high‑quality molecular diagnosis. These kits include versions for both dermatophyte species identification (DermaGenius® 2.0 and 3.0) and for the identification of resistance markers in Trichophyton (DermaGenius® Resistance). They are validated for use on nail, skin, and hair samples.
• DermaGenius® 2.0/3.0 Complete: allow detection and differentiation of the most common dermatophyte species, such as Trichophyton rubrum, T. mentagrophytes, Microsporum canis, among others. The 3.0 kits include new targets (such as T. benhamiae and E. floccosum) and greater identification accuracy, especially with the use of the new kit versions.
• DermaGenius® Resistance (PN-303): identifies the most important resistance markers in the SQLE gene, detecting specific mutations in genes that confer resistance to azole treatments, which is crucial for personalizing antifungal therapy.
The DNA extraction process is carried out using the PathoNostics Extraction Kit (PN-502), which facilitates rapid sample preparation, followed by real‑time PCR amplification to provide results within two hours. These assays are ideal for laboratories seeking to improve accuracy and speed in the diagnosis of dermatophytic infections.
The kits are validated on various real‑time PCR thermal cyclers, ensuring their versatility in different laboratory settings.
Key Features
Detection and differentiation of the main dermatophyte species: T. rubrum, T. mentagrophytes, M. canis, among others
Identification of resistance markers in the SQLE gene
Results in less than 2 hours
Compatible with nail, skin, and hair samples
Validated on several real‑time PCR systems
Use of PathoNostics Extraction Kit (PN-502) for DNA extraction
Presentation Details
DermaGenius® 2.0 Complete (PN-401)
Content: 50 reactions
Targets: C. albicans, C. parapsilosis, S. brevicaulis, T. interdigitale, T. mentagrophytes, T. schoenleinii, T. tonsurans, T. rubrum, T. violaceum, and internal control
Compatible instruments: LightCycler® 480 II, Rotor-Gene Q, QuantStudio™ 5, CFX96, Mic qPCR
CE-IVDD
DermaGenius® 3.0 Complete (PN-402)
Content: 50 reactions
Targets: T. benhamiae, T. verrucosum, M. canis, M. audouinii, E. floccosum, N. gypsae, and internal control
Compatible instruments: LightCycler® 480 II, Rotor-Gene Q, QuantStudio™ 5, CFX96, Mic qPCR
CE-IVDD
DermaGenius® Resistance (PN-303)
Content: 25 reactions
Targets: SQLE resistance markers (mutations 393 and 397), T. interdigitale, T. mentagrophytes, T. rubrum, T. violaceum, and internal control
Compatible instruments: LightCycler® 480 II, Rotor-Gene Q, QuantStudio™ 5, CFX96, Mic qPCR
CE-IVDD
DermaGenius® 3.0 Nail (PN-302)
Content: 50 reactions
Targets: Trichophyton rubrum, Trichophyton interdigitale, Candida albicans, Candida parapsilosis, Scopulariopsis brevicaulis, Internal Control
Compatible instruments: LightCycler® 480 II, Rotor-Gene Q, QuantStudio™ 5, CFX96, Mic qPCR
RUO
Extraction Kit (PN-502)
Content: 50 reactions
Use for DNA extraction in dermatophyte samples
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