HLA Fluogene

FluoGene is a unique method for molecular HLA-, RBC– (blood group) and HPA-typing combining all advantages of the SSP-PCR with the speed of endpoint fluorescence detection.

The analysis is based on a specifically modified TaqMan® probe system by inno-train. There are two options for detection: the FluoVista analyzer or the FluoQube – inno-train’s real-time device.

  • Up to four fluorophores are detected by the FluoVista® analyzer and an integrated data evaluation is realized by the FluoGene® software.
  • FluoQube® combines the FluoGene® method with real-time PCR. This system allows amplification and fluorescence reading in one system. The real-time data are automatically imported into the FluoGene® Software for result evaluation.

Inno-train’s HLA-FluoGene test systems enable typing of HLA Class I (HLA-A, -B, -C) as well as HLA Class II (HLA-DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1, -DPB1) markers that are comparable with serology.

HLA Match 384 Screenshot Lot0221 1

The new HLA FluoGene Match (SABR: single antigen bead resolution) is an ideal solution for matching donors and recipients in solid organ transplantation with a resolution comparable to single antigen antibody kits.

All FluoGene kits are CE marked in accordance with the IVD Directive 98/79/EC  and manufactured and tested under a quality system in compliance with DIN EN ISO 13485.

ADVANTAGES:

  • PCR-SSP method without gel electrophoresis within 90 minutes
  • no hybridization and washing steps
  • display of typing results immediately after the post-read
  • no risk of post-PCR contaminations
  • low DNA consumption
  • user-friendly software solution, fully-automated calculation of results
  • same system for HLA-, RBC- and HPA
  • use of patented fluorescent dyes and quencher
  • Innotrain fluogene CARRUSEL 5
  • Innotrain fluogene CARRUSEL 7
  • Innotrain fluogene CARRUSEL 2
  • Innotrain fluogene CARRUSEL 6

Area:

Immunology, Medium-low resolution HLA typing

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